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A biochanin-enriched isoflavone from red clover lowers LDL cholesterol in men.
Eur J Clin Nutr. 2004 Mar; 58(3):403-8.EJ

Abstract

OBJECTIVE

To determine whether the two major isoflavones in red clover differ in their effect on low-density lipoprotein cholesterol (LDL-C).

DESIGN

A randomised, placebo-controlled, double-blind trial; two parallel groups taking one of the two isoflavones within which treatment and placebo were administered in a crossover design.

SETTING

Free-living volunteers.

SUBJECTS

A total of 46 middle-aged men and 34 postmenopausal women.

INTERVENTION

Two mixtures of red clover isoflavones enriched in either biochanin (n=40) or formononetin (n=40) were compared. Placebo and active treatment (40 mg/day) were administered for 6 weeks each in a crossover design within the two parallel groups.

MAIN OUTCOME MEASURES

Plasma lipids were measured twice at the end of each period.

RESULTS

Baseline LDL-C concentrations did not differ significantly between men (n=46) and women (n=34), nor between those randomised to biochanin or formononetin. Interaction between time and treatments, biochanin, formononetin and corresponding placebos (two-way ANOVA) on LDL-C showed a significant effect of biochanin treatment alone. The biochanin effect was confined to men; median LDL-C was 3.61 (3.05-4.14) mmol/l with biochanin and 3.99 (3.16-4.29) mmol/l with the corresponding placebo (RM ANOVA with Dunnett's adjustment P<0.05). The difference between placebo and biochanin effects on LDL-C was 9.5%. No other lipid was affected and women failed to respond significantly to treatment.

CONCLUSION

Isolated isoflavones from red clover enriched in biochanin (genistein precursor) but not in formononetin (daidzein precursor), lowered LDL-C in men. This may partly explain the previous failure to demonstrate cholesterol-lowering effects with mixed isoflavones studied predominantly in women.

SPONSORSHIP

Novogen Ltd, North Ryde NSW, Australia, provided partial support including provision of tablets and outside monitoring.

Authors+Show Affiliations

Baker Medical Research Institute Wynn Domain, Monash University, Melbourne, Australia. paul.neslet@baker.edu.auNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Clinical Trial
Journal Article
Randomized Controlled Trial

Language

eng

PubMed ID

14985677

Citation

Nestel, P, et al. "A Biochanin-enriched Isoflavone From Red Clover Lowers LDL Cholesterol in Men." European Journal of Clinical Nutrition, vol. 58, no. 3, 2004, pp. 403-8.
Nestel P, Cehun M, Chronopoulos A, et al. A biochanin-enriched isoflavone from red clover lowers LDL cholesterol in men. Eur J Clin Nutr. 2004;58(3):403-8.
Nestel, P., Cehun, M., Chronopoulos, A., DaSilva, L., Teede, H., & McGrath, B. (2004). A biochanin-enriched isoflavone from red clover lowers LDL cholesterol in men. European Journal of Clinical Nutrition, 58(3), 403-8.
Nestel P, et al. A Biochanin-enriched Isoflavone From Red Clover Lowers LDL Cholesterol in Men. Eur J Clin Nutr. 2004;58(3):403-8. PubMed PMID: 14985677.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A biochanin-enriched isoflavone from red clover lowers LDL cholesterol in men. AU - Nestel,P, AU - Cehun,M, AU - Chronopoulos,A, AU - DaSilva,L, AU - Teede,H, AU - McGrath,B, PY - 2004/2/27/pubmed PY - 2004/6/30/medline PY - 2004/2/27/entrez SP - 403 EP - 8 JF - European journal of clinical nutrition JO - Eur J Clin Nutr VL - 58 IS - 3 N2 - OBJECTIVE: To determine whether the two major isoflavones in red clover differ in their effect on low-density lipoprotein cholesterol (LDL-C). DESIGN: A randomised, placebo-controlled, double-blind trial; two parallel groups taking one of the two isoflavones within which treatment and placebo were administered in a crossover design. SETTING: Free-living volunteers. SUBJECTS: A total of 46 middle-aged men and 34 postmenopausal women. INTERVENTION: Two mixtures of red clover isoflavones enriched in either biochanin (n=40) or formononetin (n=40) were compared. Placebo and active treatment (40 mg/day) were administered for 6 weeks each in a crossover design within the two parallel groups. MAIN OUTCOME MEASURES: Plasma lipids were measured twice at the end of each period. RESULTS: Baseline LDL-C concentrations did not differ significantly between men (n=46) and women (n=34), nor between those randomised to biochanin or formononetin. Interaction between time and treatments, biochanin, formononetin and corresponding placebos (two-way ANOVA) on LDL-C showed a significant effect of biochanin treatment alone. The biochanin effect was confined to men; median LDL-C was 3.61 (3.05-4.14) mmol/l with biochanin and 3.99 (3.16-4.29) mmol/l with the corresponding placebo (RM ANOVA with Dunnett's adjustment P<0.05). The difference between placebo and biochanin effects on LDL-C was 9.5%. No other lipid was affected and women failed to respond significantly to treatment. CONCLUSION: Isolated isoflavones from red clover enriched in biochanin (genistein precursor) but not in formononetin (daidzein precursor), lowered LDL-C in men. This may partly explain the previous failure to demonstrate cholesterol-lowering effects with mixed isoflavones studied predominantly in women. SPONSORSHIP: Novogen Ltd, North Ryde NSW, Australia, provided partial support including provision of tablets and outside monitoring. SN - 0954-3007 UR - https://www.unboundmedicine.com/medline/citation/14985677/A_biochanin_enriched_isoflavone_from_red_clover_lowers_LDL_cholesterol_in_men_ L2 - http://dx.doi.org/10.1038/sj.ejcn.1601796 DB - PRIME DP - Unbound Medicine ER -