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The molecular properties and applications of Anthozoa fluorescent proteins and chromoproteins.
Nat Biotechnol. 2004 Mar; 22(3):289-96.NBio

Abstract

The green fluorescent protein (GFP) from the jellyfish Aequorea victoria and its fluorescent homologs from Anthozoa corals have become invaluable tools for in vivo imaging of cells and tissues. Despite spectral and chromophore diversity, about 100 cloned members of the GFP-like protein family possess common structural, biochemical and photophysical features. Anthozoa GFP-like proteins are available in colors and properties unlike those of A. victoria GFP variants and thus provide powerful new fluorophores for molecular labeling and intracellular detection. Although Anthozoa GFP-like proteins provide some advantages over GFP, they also have certain drawbacks, such as obligate oligomerization and slow or incomplete fluorescence maturation. In the past few years, effective approaches for eliminating some of these limitations have been described. In addition, several Anthozoa GFP-like proteins have been developed into novel imaging agents, such as monomeric red and dimeric far-red fluorescent proteins, fluorescent timers and photoconvertible fluorescent labels. Future studies on the structure of this diverse set of proteins will further enhance their use in animal tissues and as intracellular biosensors.

Authors+Show Affiliations

Department of Pharmacology, University of Colorado Health Sciences Center, 4200 East Ninth Avenue, C236, Denver, Colorado 80262, USA. vlad.verkhusha@uchsc.eduNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.
Review

Language

eng

PubMed ID

14990950

Citation

Verkhusha, Vladislav V., and Konstantin A. Lukyanov. "The Molecular Properties and Applications of Anthozoa Fluorescent Proteins and Chromoproteins." Nature Biotechnology, vol. 22, no. 3, 2004, pp. 289-96.
Verkhusha VV, Lukyanov KA. The molecular properties and applications of Anthozoa fluorescent proteins and chromoproteins. Nat Biotechnol. 2004;22(3):289-96.
Verkhusha, V. V., & Lukyanov, K. A. (2004). The molecular properties and applications of Anthozoa fluorescent proteins and chromoproteins. Nature Biotechnology, 22(3), 289-96.
Verkhusha VV, Lukyanov KA. The Molecular Properties and Applications of Anthozoa Fluorescent Proteins and Chromoproteins. Nat Biotechnol. 2004;22(3):289-96. PubMed PMID: 14990950.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The molecular properties and applications of Anthozoa fluorescent proteins and chromoproteins. AU - Verkhusha,Vladislav V, AU - Lukyanov,Konstantin A, PY - 2004/3/3/pubmed PY - 2004/10/6/medline PY - 2004/3/3/entrez SP - 289 EP - 96 JF - Nature biotechnology JO - Nat. Biotechnol. VL - 22 IS - 3 N2 - The green fluorescent protein (GFP) from the jellyfish Aequorea victoria and its fluorescent homologs from Anthozoa corals have become invaluable tools for in vivo imaging of cells and tissues. Despite spectral and chromophore diversity, about 100 cloned members of the GFP-like protein family possess common structural, biochemical and photophysical features. Anthozoa GFP-like proteins are available in colors and properties unlike those of A. victoria GFP variants and thus provide powerful new fluorophores for molecular labeling and intracellular detection. Although Anthozoa GFP-like proteins provide some advantages over GFP, they also have certain drawbacks, such as obligate oligomerization and slow or incomplete fluorescence maturation. In the past few years, effective approaches for eliminating some of these limitations have been described. In addition, several Anthozoa GFP-like proteins have been developed into novel imaging agents, such as monomeric red and dimeric far-red fluorescent proteins, fluorescent timers and photoconvertible fluorescent labels. Future studies on the structure of this diverse set of proteins will further enhance their use in animal tissues and as intracellular biosensors. SN - 1087-0156 UR - https://www.unboundmedicine.com/medline/citation/14990950/The_molecular_properties_and_applications_of_Anthozoa_fluorescent_proteins_and_chromoproteins_ L2 - https://dx.doi.org/10.1038/nbt943 DB - PRIME DP - Unbound Medicine ER -