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Mutagenicity of carbon tetrachloride and chloroform in Salmonella typhimurium TA98, TA100, TA1535, and TA1537, and Escherichia coli WP2uvrA/pKM101 and WP2/pKM101, using a gas exposure method.
Environ Mol Mutagen. 2004; 43(2):128-33.EM

Abstract

The volatile solvents carbon tetrachloride and chloroform are carcinogens that are often reported as nonmutagenic in bacterial mutagenicity assays. In this study, we evaluated the mutagenicity of these compounds in Salmonella typhimurium TA98, TA100, TA1535, and TA1537, and Escherichia coli WP2uvrA/pKM101 and WP2/pKM101, with and without S9 mix, using a gas exposure method. Tests were also conducted with a glutathione-supplemented S9 mix. Carbon tetrachloride was mutagenic in TA98 without S9 mix, and in WP2/pKM101 and WP2uvrA/pKM101 with and without S9 mix; carbon tetrachloride was not mutagenic in TA100, TA1535 or TA1537. Chloroform was mutagenic in WP2/pKM101, but only in the presence of glutathione-supplemented S9 mix. Chloroform was not mutagenic in TA98, TA100, TA1535, TA1537, or WP2uvrA/pKM101 with or without S9 mix, and was not mutagenic in TA98, TA100, TA1535, TA1537, or WP2uvrA/pKM101 in the presence of glutathione-supplemented S9 mix. The data indicate that carbon tetrachloride and chloroform are bacterial mutagens when adequate exposure conditions are employed and suggest that a genotoxic mode of action could contribute to the carcinogenicity of these compounds.

Authors+Show Affiliations

Araki A
Mutagenicity Test Division, Japan Bioassay Research Center, Japan Industrial Safety and Health Association, Hadano, Kanagawa, Japan. aaraki@jisha.or.jp
Kamigaito N
No affiliation info available
Sasaki T
No affiliation info available
Matsushima T
No affiliation info available

MeSH

AnimalsBiotransformationCarbon TetrachlorideChloroformEscherichia coliMaleMicrosomes, LiverMutagenicity TestsMutagensRatsRats, Sprague-DawleySalmonella typhimuriumSpecies Specificity

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

14991753

Citation

Araki, Akihiro, et al. "Mutagenicity of Carbon Tetrachloride and Chloroform in Salmonella Typhimurium TA98, TA100, TA1535, and TA1537, and Escherichia Coli WP2uvrA/pKM101 and WP2/pKM101, Using a Gas Exposure Method." Environmental and Molecular Mutagenesis, vol. 43, no. 2, 2004, pp. 128-33.
Araki A, Kamigaito N, Sasaki T, et al. Mutagenicity of carbon tetrachloride and chloroform in Salmonella typhimurium TA98, TA100, TA1535, and TA1537, and Escherichia coli WP2uvrA/pKM101 and WP2/pKM101, using a gas exposure method. Environ Mol Mutagen. 2004;43(2):128-33.
Araki, A., Kamigaito, N., Sasaki, T., & Matsushima, T. (2004). Mutagenicity of carbon tetrachloride and chloroform in Salmonella typhimurium TA98, TA100, TA1535, and TA1537, and Escherichia coli WP2uvrA/pKM101 and WP2/pKM101, using a gas exposure method. Environmental and Molecular Mutagenesis, 43(2), 128-33.
Araki A, et al. Mutagenicity of Carbon Tetrachloride and Chloroform in Salmonella Typhimurium TA98, TA100, TA1535, and TA1537, and Escherichia Coli WP2uvrA/pKM101 and WP2/pKM101, Using a Gas Exposure Method. Environ Mol Mutagen. 2004;43(2):128-33. PubMed PMID: 14991753.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Mutagenicity of carbon tetrachloride and chloroform in Salmonella typhimurium TA98, TA100, TA1535, and TA1537, and Escherichia coli WP2uvrA/pKM101 and WP2/pKM101, using a gas exposure method. AU - Araki,Akihiro, AU - Kamigaito,Nobuyuki, AU - Sasaki,Toshiaki, AU - Matsushima,Taijiro, PY - 2004/3/3/pubmed PY - 2004/4/13/medline PY - 2004/3/3/entrez SP - 128 EP - 33 JF - Environmental and molecular mutagenesis JO - Environ Mol Mutagen VL - 43 IS - 2 N2 - The volatile solvents carbon tetrachloride and chloroform are carcinogens that are often reported as nonmutagenic in bacterial mutagenicity assays. In this study, we evaluated the mutagenicity of these compounds in Salmonella typhimurium TA98, TA100, TA1535, and TA1537, and Escherichia coli WP2uvrA/pKM101 and WP2/pKM101, with and without S9 mix, using a gas exposure method. Tests were also conducted with a glutathione-supplemented S9 mix. Carbon tetrachloride was mutagenic in TA98 without S9 mix, and in WP2/pKM101 and WP2uvrA/pKM101 with and without S9 mix; carbon tetrachloride was not mutagenic in TA100, TA1535 or TA1537. Chloroform was mutagenic in WP2/pKM101, but only in the presence of glutathione-supplemented S9 mix. Chloroform was not mutagenic in TA98, TA100, TA1535, TA1537, or WP2uvrA/pKM101 with or without S9 mix, and was not mutagenic in TA98, TA100, TA1535, TA1537, or WP2uvrA/pKM101 in the presence of glutathione-supplemented S9 mix. The data indicate that carbon tetrachloride and chloroform are bacterial mutagens when adequate exposure conditions are employed and suggest that a genotoxic mode of action could contribute to the carcinogenicity of these compounds. SN - 0893-6692 UR - https://www.unboundmedicine.com/medline/citation/14991753/Mutagenicity_of_carbon_tetrachloride_and_chloroform_in_Salmonella_typhimurium_TA98_TA100_TA1535_and_TA1537_and_Escherichia_coli_WP2uvrA/pKM101_and_WP2/pKM101_using_a_gas_exposure_method_ DB - PRIME DP - Unbound Medicine ER -