Tags

Type your tag names separated by a space and hit enter

Crystallization and preliminary X-ray analysis of inorganic pyrophosphatase from the hyperthermophilic archaeon Pyrococcus horikoshii OT3.
Acta Crystallogr D Biol Crystallogr. 2004 Mar; 60(Pt 3):577-9.AC

Abstract

Inorganic pyrophosphatase (PPase; EC 3.6.1.1) from the hyperthermophile Pyrococcus horikoshii was crystallized by the hanging-drop vapour-diffusion method at pH 5.0 using polyethyleneglycol 4000 as the precipitant. The crystal belongs to space group P2(1)2(1)2, with unit-cell parameters a = 71.7, b = 86.5, c = 92.5 A, alpha = beta = gamma = 90 degrees. There are two molecules in the asymmetric unit. The crystals were stable during exposure to X-rays and a full set of X-ray diffraction data was collected to 2.7 A resolution in-house.

Authors+Show Affiliations

Liu B
Laboratory of Structural Biology, Department of Biological Science and Biotechnology, Tsinghua University, Beijing 100084, People's Republic of China.
Li X
No affiliation info available
Gao R
No affiliation info available
Zhou W
No affiliation info available
Xie G
No affiliation info available
Bartlam M
No affiliation info available
Pang H
No affiliation info available
Feng Y
No affiliation info available
Rao Z
No affiliation info available

MeSH

CrystallizationCrystallography, X-RayHydrogen-Ion ConcentrationInorganic PyrophosphatasePolyethylene GlycolsPyrococcus horikoshiiRecombinant Proteins

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

14993699

Citation

Liu, Binbin, et al. "Crystallization and Preliminary X-ray Analysis of Inorganic Pyrophosphatase From the Hyperthermophilic Archaeon Pyrococcus Horikoshii OT3." Acta Crystallographica. Section D, Biological Crystallography, vol. 60, no. Pt 3, 2004, pp. 577-9.
Liu B, Li X, Gao R, et al. Crystallization and preliminary X-ray analysis of inorganic pyrophosphatase from the hyperthermophilic archaeon Pyrococcus horikoshii OT3. Acta Crystallogr D Biol Crystallogr. 2004;60(Pt 3):577-9.
Liu, B., Li, X., Gao, R., Zhou, W., Xie, G., Bartlam, M., Pang, H., Feng, Y., & Rao, Z. (2004). Crystallization and preliminary X-ray analysis of inorganic pyrophosphatase from the hyperthermophilic archaeon Pyrococcus horikoshii OT3. Acta Crystallographica. Section D, Biological Crystallography, 60(Pt 3), 577-9.
Liu B, et al. Crystallization and Preliminary X-ray Analysis of Inorganic Pyrophosphatase From the Hyperthermophilic Archaeon Pyrococcus Horikoshii OT3. Acta Crystallogr D Biol Crystallogr. 2004;60(Pt 3):577-9. PubMed PMID: 14993699.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Crystallization and preliminary X-ray analysis of inorganic pyrophosphatase from the hyperthermophilic archaeon Pyrococcus horikoshii OT3. AU - Liu,Binbin, AU - Li,Xuemei, AU - Gao,Renjun, AU - Zhou,Weihong, AU - Xie,Guiqiu, AU - Bartlam,Mark, AU - Pang,Hai, AU - Feng,Yan, AU - Rao,Zihe, Y1 - 2004/02/25/ PY - 2003/06/01/received PY - 2004/01/08/accepted PY - 2004/3/3/pubmed PY - 2004/11/13/medline PY - 2004/3/3/entrez SP - 577 EP - 9 JF - Acta crystallographica. Section D, Biological crystallography JO - Acta Crystallogr D Biol Crystallogr VL - 60 IS - Pt 3 N2 - Inorganic pyrophosphatase (PPase; EC 3.6.1.1) from the hyperthermophile Pyrococcus horikoshii was crystallized by the hanging-drop vapour-diffusion method at pH 5.0 using polyethyleneglycol 4000 as the precipitant. The crystal belongs to space group P2(1)2(1)2, with unit-cell parameters a = 71.7, b = 86.5, c = 92.5 A, alpha = beta = gamma = 90 degrees. There are two molecules in the asymmetric unit. The crystals were stable during exposure to X-rays and a full set of X-ray diffraction data was collected to 2.7 A resolution in-house. SN - 0907-4449 UR - https://www.unboundmedicine.com/medline/citation/14993699/Crystallization_and_preliminary_X_ray_analysis_of_inorganic_pyrophosphatase_from_the_hyperthermophilic_archaeon_Pyrococcus_horikoshii_OT3_ DB - PRIME DP - Unbound Medicine ER -