Tags

Type your tag names separated by a space and hit enter

Ganglioside GM1 levels are a determinant of the extent of caveolae/raft-dependent endocytosis of cholera toxin to the Golgi apparatus.
J Cell Sci. 2004 Mar 15; 117(Pt 8):1421-30.JC

Abstract

Cholera toxin is associated with caveolae and raft domains in various cell types and previous studies have shown that cholera toxin can be internalized by caveolae/raft-dependent endocytosis as well as by other pathways. We undertook the study of cholera toxin endocytosis in CaCo-2 and HeLa cells. CaCo-2 cells do not express detectable levels of caveolin and, relative to HeLa cells, also present significantly reduced expression of ganglioside GM1, the cholera toxin receptor, that remains Triton X-100 insoluble. Amongst the HeLa cell population, caveolin expression is constant, however, GM1 expression is highly variable. Cholera toxin is internalized to the Golgi apparatus via a caveolae/raft-dependent pathway sensitive to methyl-beta-cyclodextrin and genistein in high-GM1-expressing HeLa cells but not in low-GM1 HeLa cells or in CaCo-2 cells. Limited cholera toxin endocytosis to endosomes sensitive to neither methyl-beta-cyclodextrin nor genistein is also observed in all cells and corresponds to a non-caveolae/raft endocytic pathway. Increasing cell-associated GM1 by adding GM1 to the cell media of both HeLa and CaCo-2 cells selectively enhances the methyl-beta-cyclodextrin-, genistein-sensitive delivery of cholera toxin to the Golgi apparatus but not to endosomes. GM1 expression levels are therefore a selective determinant of caveolae/raft-dependent endocytosis of cholera toxin to the Golgi apparatus and variable expression of GM1 between cells can impact on the endocytosis and choice of pathway followed by cholera toxin.

Authors+Show Affiliations

Department of Pathology and Cell Biology, Université de Montréal, Montreal, Quebec, Canada H3C 3J7.No affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article

Language

eng

PubMed ID

14996913

Citation

Pang, Hao, et al. "Ganglioside GM1 Levels Are a Determinant of the Extent of Caveolae/raft-dependent Endocytosis of Cholera Toxin to the Golgi Apparatus." Journal of Cell Science, vol. 117, no. Pt 8, 2004, pp. 1421-30.
Pang H, Le PU, Nabi IR. Ganglioside GM1 levels are a determinant of the extent of caveolae/raft-dependent endocytosis of cholera toxin to the Golgi apparatus. J Cell Sci. 2004;117(Pt 8):1421-30.
Pang, H., Le, P. U., & Nabi, I. R. (2004). Ganglioside GM1 levels are a determinant of the extent of caveolae/raft-dependent endocytosis of cholera toxin to the Golgi apparatus. Journal of Cell Science, 117(Pt 8), 1421-30.
Pang H, Le PU, Nabi IR. Ganglioside GM1 Levels Are a Determinant of the Extent of Caveolae/raft-dependent Endocytosis of Cholera Toxin to the Golgi Apparatus. J Cell Sci. 2004 Mar 15;117(Pt 8):1421-30. PubMed PMID: 14996913.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Ganglioside GM1 levels are a determinant of the extent of caveolae/raft-dependent endocytosis of cholera toxin to the Golgi apparatus. AU - Pang,Hao, AU - Le,Phuong U, AU - Nabi,Ivan R, Y1 - 2004/03/02/ PY - 2004/3/5/pubmed PY - 2004/12/16/medline PY - 2004/3/5/entrez SP - 1421 EP - 30 JF - Journal of cell science JO - J. Cell. Sci. VL - 117 IS - Pt 8 N2 - Cholera toxin is associated with caveolae and raft domains in various cell types and previous studies have shown that cholera toxin can be internalized by caveolae/raft-dependent endocytosis as well as by other pathways. We undertook the study of cholera toxin endocytosis in CaCo-2 and HeLa cells. CaCo-2 cells do not express detectable levels of caveolin and, relative to HeLa cells, also present significantly reduced expression of ganglioside GM1, the cholera toxin receptor, that remains Triton X-100 insoluble. Amongst the HeLa cell population, caveolin expression is constant, however, GM1 expression is highly variable. Cholera toxin is internalized to the Golgi apparatus via a caveolae/raft-dependent pathway sensitive to methyl-beta-cyclodextrin and genistein in high-GM1-expressing HeLa cells but not in low-GM1 HeLa cells or in CaCo-2 cells. Limited cholera toxin endocytosis to endosomes sensitive to neither methyl-beta-cyclodextrin nor genistein is also observed in all cells and corresponds to a non-caveolae/raft endocytic pathway. Increasing cell-associated GM1 by adding GM1 to the cell media of both HeLa and CaCo-2 cells selectively enhances the methyl-beta-cyclodextrin-, genistein-sensitive delivery of cholera toxin to the Golgi apparatus but not to endosomes. GM1 expression levels are therefore a selective determinant of caveolae/raft-dependent endocytosis of cholera toxin to the Golgi apparatus and variable expression of GM1 between cells can impact on the endocytosis and choice of pathway followed by cholera toxin. SN - 0021-9533 UR - https://www.unboundmedicine.com/medline/citation/14996913/Ganglioside_GM1_levels_are_a_determinant_of_the_extent_of_caveolae/raft_dependent_endocytosis_of_cholera_toxin_to_the_Golgi_apparatus_ L2 - http://jcs.biologists.org/cgi/pmidlookup?view=long&pmid=14996913 DB - PRIME DP - Unbound Medicine ER -