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Exhaled breath condensate eicosanoids and sputum eosinophils in asthmatic children: a pilot study.
Pediatr Allergy Immunol. 2004 Feb; 15(1):26-31.PA

Abstract

Cysteinyl leukotrienes (cys-LTs), LTB4 and 8-isoprostane are increased in the exhaled breath condensate (EBC) from asthmatic patients. The aim of this study was to investigate whether the measurement of cys-LTs, LTB4 and 8-isoprostane in EBC can reflect the level of airway inflammation assessed by induced sputum in asthmatic children sensitized to house dust mite (HDM) during natural avoidance of HDM allergens. Twelve children were evaluated at the time of admission (T0) and after 3 months of stay (T1) at the Istituto Pio XII (Misurina, Italian Dolomites 1756 m). Sputum eosinophil percentage and measurement of cys-LTs, LTB4 and 8-isoprostanes in the breath condensate at T0 and T1 were evaluated. Eosinophil percentage in induced sputum was 8.5 +/- 1.1% at T0 and 3.5 +/- 0.4% at T1 (p = 0.011). Neutrophil percentage in sputum was 1.1 +/- 0.5% at T0 and 1.5 +/- 1.0% at T1 (ns). Cys-LTs mean level was 14.24 +/- 4.53 pg/ml at T0 and 4.65 +/- 0.68 pg/ml at T1 (p = 0.0125). LTB4 level was 2.36 +/- 0.19 pg/ml at T0 and 2.41 +/- 0.23 pg/ml at T1 (ns). 8-Isoprostane level reduced from 17.47 +/- 3.18 pg/ml at T0 to 7.36 +/- 3.26 pg/ml at T1 (p = 0.003). This study show that exhaled cys-LTs and 8-isoprostane, as well as eosinophil percentage in induced sputum, are reduced after allergen avoidance in asthmatic children suggesting a potential application of EBC for the non-invasive evaluation of airway inflammation in asthma in allergic asthmatic children.

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Authors+Show Affiliations

Bodini A
Clinica Pediatrica, Università di Verona, Verona, Italy.
Peroni D
No affiliation info available
Vicentini L
No affiliation info available
Loiacono A
No affiliation info available
Baraldi E
No affiliation info available
Ghiro L
No affiliation info available
Corradi M
No affiliation info available
Alinovi R
No affiliation info available
Boner AL
No affiliation info available
Piacentini GL
No affiliation info available

MeSH

AdolescentAltitudeAsthmaBreath TestsChildCysteineEicosanoidsEosinophilsFemaleHumansLeukotriene B4LeukotrienesMalePilot ProjectsProstaglandins APyroglyphidaeSputum

Pub Type(s)

Journal Article

Language

eng

PubMed ID

14998379

Citation

Bodini, A, et al. "Exhaled Breath Condensate Eicosanoids and Sputum Eosinophils in Asthmatic Children: a Pilot Study." Pediatric Allergy and Immunology : Official Publication of the European Society of Pediatric Allergy and Immunology, vol. 15, no. 1, 2004, pp. 26-31.
Bodini A, Peroni D, Vicentini L, et al. Exhaled breath condensate eicosanoids and sputum eosinophils in asthmatic children: a pilot study. Pediatr Allergy Immunol. 2004;15(1):26-31.
Bodini, A., Peroni, D., Vicentini, L., Loiacono, A., Baraldi, E., Ghiro, L., Corradi, M., Alinovi, R., Boner, A. L., & Piacentini, G. L. (2004). Exhaled breath condensate eicosanoids and sputum eosinophils in asthmatic children: a pilot study. Pediatric Allergy and Immunology : Official Publication of the European Society of Pediatric Allergy and Immunology, 15(1), 26-31.
Bodini A, et al. Exhaled Breath Condensate Eicosanoids and Sputum Eosinophils in Asthmatic Children: a Pilot Study. Pediatr Allergy Immunol. 2004;15(1):26-31. PubMed PMID: 14998379.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Exhaled breath condensate eicosanoids and sputum eosinophils in asthmatic children: a pilot study. AU - Bodini,A, AU - Peroni,D, AU - Vicentini,L, AU - Loiacono,A, AU - Baraldi,E, AU - Ghiro,L, AU - Corradi,M, AU - Alinovi,R, AU - Boner,A L, AU - Piacentini,G L, PY - 2004/3/5/pubmed PY - 2004/7/2/medline PY - 2004/3/5/entrez SP - 26 EP - 31 JF - Pediatric allergy and immunology : official publication of the European Society of Pediatric Allergy and Immunology JO - Pediatr Allergy Immunol VL - 15 IS - 1 N2 - Cysteinyl leukotrienes (cys-LTs), LTB4 and 8-isoprostane are increased in the exhaled breath condensate (EBC) from asthmatic patients. The aim of this study was to investigate whether the measurement of cys-LTs, LTB4 and 8-isoprostane in EBC can reflect the level of airway inflammation assessed by induced sputum in asthmatic children sensitized to house dust mite (HDM) during natural avoidance of HDM allergens. Twelve children were evaluated at the time of admission (T0) and after 3 months of stay (T1) at the Istituto Pio XII (Misurina, Italian Dolomites 1756 m). Sputum eosinophil percentage and measurement of cys-LTs, LTB4 and 8-isoprostanes in the breath condensate at T0 and T1 were evaluated. Eosinophil percentage in induced sputum was 8.5 +/- 1.1% at T0 and 3.5 +/- 0.4% at T1 (p = 0.011). Neutrophil percentage in sputum was 1.1 +/- 0.5% at T0 and 1.5 +/- 1.0% at T1 (ns). Cys-LTs mean level was 14.24 +/- 4.53 pg/ml at T0 and 4.65 +/- 0.68 pg/ml at T1 (p = 0.0125). LTB4 level was 2.36 +/- 0.19 pg/ml at T0 and 2.41 +/- 0.23 pg/ml at T1 (ns). 8-Isoprostane level reduced from 17.47 +/- 3.18 pg/ml at T0 to 7.36 +/- 3.26 pg/ml at T1 (p = 0.003). This study show that exhaled cys-LTs and 8-isoprostane, as well as eosinophil percentage in induced sputum, are reduced after allergen avoidance in asthmatic children suggesting a potential application of EBC for the non-invasive evaluation of airway inflammation in asthma in allergic asthmatic children. SN - 0905-6157 UR - https://www.unboundmedicine.com/medline/citation/14998379/Exhaled_breath_condensate_eicosanoids_and_sputum_eosinophils_in_asthmatic_children:_a_pilot_study_ L2 - https://onlinelibrary.wiley.com/resolve/openurl?genre=article&sid=nlm:pubmed&issn=0905-6157&date=2004&volume=15&issue=1&spage=26 DB - PRIME DP - Unbound Medicine ER -