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Branched-chain amino acids increase p70S6k phosphorylation in human skeletal muscle after resistance exercise.
Am J Physiol Endocrinol Metab. 2004 Jul; 287(1):E1-7.AJ

Abstract

The aim of the study was to investigate the effect of resistance exercise alone or in combination with oral intake of branched-chain amino acids (BCAA) on phosphorylation of the 70-kDa S6 protein kinase (p70(S6k)) and mitogen-activated protein kinase (MAPK), extracellular signal-regulated kinase (ERK1/2), and p38 MAPK in skeletal muscle. Seven male subjects performed one session of quadriceps muscle resistance training (4 x 10 repetitions at 80% of one repetition maximum) on two occasions. In a randomized order, double-blind, crossover test, subjects ingested a solution of BCAA or placebo during and after exercise. Ingestion of BCAA increased plasma concentrations of isoleucine, leucine, and valine during exercise and throughout recovery after exercise (2 h postexercise), whereas no change was noted after the placebo trial. Resistance exercise led to a robust increase in p70(S6k) phosphorylation at Ser(424) and/or Thr(421), which persisted 1 and 2 h after exercise. BCAA ingestion further enhanced p70(S6k) phosphorylation 3.5-fold during recovery. p70(S6k) phosphorylation at Thr(389) was unaltered directly after resistance exercise. However, during recovery, Thr(389) phosphorylation was profoundly increased, but only during the BCAA trial. Furthermore, phosphorylation of the ribosomal protein S6 was also increased in the recovery period only during the BCAA trial. Exercise led to a marked increase in ERK1/2 and p38 MAPK phosphorylation, which was completely suppressed upon recovery and unaltered by BCAA. In conclusion, BCAA, ingested during and after resistance exercise, mediate signal transduction through p70(S6k) in skeletal muscle.

Authors+Show Affiliations

Univ. College of Physical Education and Sports, Box 5626, S-114 86 Stockholm, Sweden.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Clinical Trial
Journal Article
Randomized Controlled Trial
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

14998784

Citation

Karlsson, Håkan K R., et al. "Branched-chain Amino Acids Increase p70S6k Phosphorylation in Human Skeletal Muscle After Resistance Exercise." American Journal of Physiology. Endocrinology and Metabolism, vol. 287, no. 1, 2004, pp. E1-7.
Karlsson HK, Nilsson PA, Nilsson J, et al. Branched-chain amino acids increase p70S6k phosphorylation in human skeletal muscle after resistance exercise. Am J Physiol Endocrinol Metab. 2004;287(1):E1-7.
Karlsson, H. K., Nilsson, P. A., Nilsson, J., Chibalin, A. V., Zierath, J. R., & Blomstrand, E. (2004). Branched-chain amino acids increase p70S6k phosphorylation in human skeletal muscle after resistance exercise. American Journal of Physiology. Endocrinology and Metabolism, 287(1), E1-7.
Karlsson HK, et al. Branched-chain Amino Acids Increase p70S6k Phosphorylation in Human Skeletal Muscle After Resistance Exercise. Am J Physiol Endocrinol Metab. 2004;287(1):E1-7. PubMed PMID: 14998784.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Branched-chain amino acids increase p70S6k phosphorylation in human skeletal muscle after resistance exercise. AU - Karlsson,Håkan K R, AU - Nilsson,Per-Anders, AU - Nilsson,Johnny, AU - Chibalin,Alexander V, AU - Zierath,Juleen R, AU - Blomstrand,Eva, Y1 - 2004/03/02/ PY - 2004/3/5/pubmed PY - 2004/7/23/medline PY - 2004/3/5/entrez SP - E1 EP - 7 JF - American journal of physiology. Endocrinology and metabolism JO - Am J Physiol Endocrinol Metab VL - 287 IS - 1 N2 - The aim of the study was to investigate the effect of resistance exercise alone or in combination with oral intake of branched-chain amino acids (BCAA) on phosphorylation of the 70-kDa S6 protein kinase (p70(S6k)) and mitogen-activated protein kinase (MAPK), extracellular signal-regulated kinase (ERK1/2), and p38 MAPK in skeletal muscle. Seven male subjects performed one session of quadriceps muscle resistance training (4 x 10 repetitions at 80% of one repetition maximum) on two occasions. In a randomized order, double-blind, crossover test, subjects ingested a solution of BCAA or placebo during and after exercise. Ingestion of BCAA increased plasma concentrations of isoleucine, leucine, and valine during exercise and throughout recovery after exercise (2 h postexercise), whereas no change was noted after the placebo trial. Resistance exercise led to a robust increase in p70(S6k) phosphorylation at Ser(424) and/or Thr(421), which persisted 1 and 2 h after exercise. BCAA ingestion further enhanced p70(S6k) phosphorylation 3.5-fold during recovery. p70(S6k) phosphorylation at Thr(389) was unaltered directly after resistance exercise. However, during recovery, Thr(389) phosphorylation was profoundly increased, but only during the BCAA trial. Furthermore, phosphorylation of the ribosomal protein S6 was also increased in the recovery period only during the BCAA trial. Exercise led to a marked increase in ERK1/2 and p38 MAPK phosphorylation, which was completely suppressed upon recovery and unaltered by BCAA. In conclusion, BCAA, ingested during and after resistance exercise, mediate signal transduction through p70(S6k) in skeletal muscle. SN - 0193-1849 UR - https://www.unboundmedicine.com/medline/citation/14998784/Branched_chain_amino_acids_increase_p70S6k_phosphorylation_in_human_skeletal_muscle_after_resistance_exercise_ L2 - https://journals.physiology.org/doi/10.1152/ajpendo.00430.2003?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -