Tags

Type your tag names separated by a space and hit enter

Regulatory role of nitric oxide over extracellular taurine in the hippocampus of freely moving rats.
Neurosci Lett. 2004 Mar 11; 357(3):179-82.NL

Abstract

We have studied the effects of drugs which manipulate nitric oxide (NO) levels as well the effect of N-methyl-d-aspartate (NMDA) infusion on extracellular taurine in rat hippocampus using in vivo microdialysis. The NO donor S-nitroso-N-acetylpenicillamine (SNAP) increased dialysate taurine in a concentration-dependent manner, and this effect was blocked by the inhibitor of soluble guanylate cyclase1H-[1,2,4]oxadiazolo[4,3-alpha]quinoxalin-1-one (ODQ). NMDA (100 microM) increased hippocampal taurine release, an effect that was reversed by the NMDA receptor antagonist 2-amino-5-phosphonopentanoic acid (AP5; 10 microM). The non-selective nitric oxide synthase (NOS) inhibitor N-nitro-l-arginine methyl ester (L-NAME; 100 microM and 1.0 mM) increased extracellular taurine in a concentration-dependent manner while 7-nitroindazole (7-NI), a relatively selective neuronal NOS (nNOS) inhibitor, at the same concentrations decreased extracellular taurine. L-NAME (1.0 mM) infused prior to NMDA did not alter the effect of NMDA on extracellular taurine having an effect essentially identical to that seen with L-NAME infused alone. In contrast, when 7-NI was infused for 30 min prior to NMDA, taurine levels were no longer increased above basal. This suggests to us that taurine efflux is mediated by two different mechanisms: an NMDA-evoked, 7-NI-sensitive pathway which may be dependent on cyclic guanosine monophosphate formation, and an L-NAME-modulated mechanism which presumably involves other members of the NOS group of enzymes than nNOS alone.

Authors+Show Affiliations

Department of Pharmacology, The School of Pharmacy, London WC1N 1AX, UK.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article

Language

eng

PubMed ID

15003279

Citation

Watts, Jo, et al. "Regulatory Role of Nitric Oxide Over Extracellular Taurine in the Hippocampus of Freely Moving Rats." Neuroscience Letters, vol. 357, no. 3, 2004, pp. 179-82.
Watts J, Segieth J, Pearce B, et al. Regulatory role of nitric oxide over extracellular taurine in the hippocampus of freely moving rats. Neurosci Lett. 2004;357(3):179-82.
Watts, J., Segieth, J., Pearce, B., & Whitton, P. S. (2004). Regulatory role of nitric oxide over extracellular taurine in the hippocampus of freely moving rats. Neuroscience Letters, 357(3), 179-82.
Watts J, et al. Regulatory Role of Nitric Oxide Over Extracellular Taurine in the Hippocampus of Freely Moving Rats. Neurosci Lett. 2004 Mar 11;357(3):179-82. PubMed PMID: 15003279.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Regulatory role of nitric oxide over extracellular taurine in the hippocampus of freely moving rats. AU - Watts,Jo, AU - Segieth,Joanna, AU - Pearce,Brian, AU - Whitton,Peter S, PY - 2003/10/23/received PY - 2003/12/15/revised PY - 2003/12/15/accepted PY - 2004/3/9/pubmed PY - 2004/4/27/medline PY - 2004/3/9/entrez SP - 179 EP - 82 JF - Neuroscience letters JO - Neurosci Lett VL - 357 IS - 3 N2 - We have studied the effects of drugs which manipulate nitric oxide (NO) levels as well the effect of N-methyl-d-aspartate (NMDA) infusion on extracellular taurine in rat hippocampus using in vivo microdialysis. The NO donor S-nitroso-N-acetylpenicillamine (SNAP) increased dialysate taurine in a concentration-dependent manner, and this effect was blocked by the inhibitor of soluble guanylate cyclase1H-[1,2,4]oxadiazolo[4,3-alpha]quinoxalin-1-one (ODQ). NMDA (100 microM) increased hippocampal taurine release, an effect that was reversed by the NMDA receptor antagonist 2-amino-5-phosphonopentanoic acid (AP5; 10 microM). The non-selective nitric oxide synthase (NOS) inhibitor N-nitro-l-arginine methyl ester (L-NAME; 100 microM and 1.0 mM) increased extracellular taurine in a concentration-dependent manner while 7-nitroindazole (7-NI), a relatively selective neuronal NOS (nNOS) inhibitor, at the same concentrations decreased extracellular taurine. L-NAME (1.0 mM) infused prior to NMDA did not alter the effect of NMDA on extracellular taurine having an effect essentially identical to that seen with L-NAME infused alone. In contrast, when 7-NI was infused for 30 min prior to NMDA, taurine levels were no longer increased above basal. This suggests to us that taurine efflux is mediated by two different mechanisms: an NMDA-evoked, 7-NI-sensitive pathway which may be dependent on cyclic guanosine monophosphate formation, and an L-NAME-modulated mechanism which presumably involves other members of the NOS group of enzymes than nNOS alone. SN - 0304-3940 UR - https://www.unboundmedicine.com/medline/citation/15003279/Regulatory_role_of_nitric_oxide_over_extracellular_taurine_in_the_hippocampus_of_freely_moving_rats_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0304394003015155 DB - PRIME DP - Unbound Medicine ER -