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Hyperlipidaemic effect of fish oil in Bio F1B hamsters.
Br J Nutr 2004; 91(3):341-9BJ

Abstract

We investigated the dietary influence of low and high levels of fish oil, supplemented with or without dietary cholesterol, on the plasma lipoprotein profile in Bio F1B hamsters, a model susceptible to diet-induced hyperlipidaemia. The MIX diet, a diet supplemented with a mixture of lard and safflower-seed oil, was used as the control diet to maintain the saturated MUFA and PUFA levels similar to the fish-oil diet. The animals were fed the specific diets for 2 weeks and fasted for 14 h before killing. The plasma from the animals fed high levels of fish oil was milky and rich in chylomicron-like particles. The plasma total cholesterol, VLDL- and LDL-cholesterol and -triacylglycerol concentrations were significantly higher, whereas HDL-cholesterol was lower in hamsters fed fish oil compared with the MIX-diet-fed hamsters. Increasing the amount of fat in the diet increased plasma lipids in both the fish-oil- and the MIX-diet-fed hamsters; however, this hyperlipidaemic effect of dietary fat level was greater in the hamsters fed the fish-oil diet. The hepatic lipid concentrations were not dramatically different between the fish-oil-fed and the MIX-diet-fed hamsters. However, the hepatic LDL-receptor mRNA levels were significantly low in the fish-oil-fed hamsters compared with the MIX-diet-fed hamsters. Increasing the amount of fish oil in the diet further decreased the hepatic LDL-receptor mRNA expression. It is concluded that F1B hamsters are susceptible to fish-oil-induced hyperlipidaemia, especially at high fat levels, and this increase is partially explained by the inhibition of hepatic LDL-receptor mRNA expression.

Authors+Show Affiliations

Department of Biochemistry, Memorial University of Newfoundland, St John's, NL, Canada A1B 3X9.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15005819

Citation

de Silva, Pujitha P., et al. "Hyperlipidaemic Effect of Fish Oil in Bio F1B Hamsters." The British Journal of Nutrition, vol. 91, no. 3, 2004, pp. 341-9.
de Silva PP, Davis PJ, Cheema SK. Hyperlipidaemic effect of fish oil in Bio F1B hamsters. Br J Nutr. 2004;91(3):341-9.
de Silva, P. P., Davis, P. J., & Cheema, S. K. (2004). Hyperlipidaemic effect of fish oil in Bio F1B hamsters. The British Journal of Nutrition, 91(3), pp. 341-9.
de Silva PP, Davis PJ, Cheema SK. Hyperlipidaemic Effect of Fish Oil in Bio F1B Hamsters. Br J Nutr. 2004;91(3):341-9. PubMed PMID: 15005819.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Hyperlipidaemic effect of fish oil in Bio F1B hamsters. AU - de Silva,Pujitha P, AU - Davis,Phillip J, AU - Cheema,Sukhinder Kaur, PY - 2004/3/10/pubmed PY - 2004/3/31/medline PY - 2004/3/10/entrez SP - 341 EP - 9 JF - The British journal of nutrition JO - Br. J. Nutr. VL - 91 IS - 3 N2 - We investigated the dietary influence of low and high levels of fish oil, supplemented with or without dietary cholesterol, on the plasma lipoprotein profile in Bio F1B hamsters, a model susceptible to diet-induced hyperlipidaemia. The MIX diet, a diet supplemented with a mixture of lard and safflower-seed oil, was used as the control diet to maintain the saturated MUFA and PUFA levels similar to the fish-oil diet. The animals were fed the specific diets for 2 weeks and fasted for 14 h before killing. The plasma from the animals fed high levels of fish oil was milky and rich in chylomicron-like particles. The plasma total cholesterol, VLDL- and LDL-cholesterol and -triacylglycerol concentrations were significantly higher, whereas HDL-cholesterol was lower in hamsters fed fish oil compared with the MIX-diet-fed hamsters. Increasing the amount of fat in the diet increased plasma lipids in both the fish-oil- and the MIX-diet-fed hamsters; however, this hyperlipidaemic effect of dietary fat level was greater in the hamsters fed the fish-oil diet. The hepatic lipid concentrations were not dramatically different between the fish-oil-fed and the MIX-diet-fed hamsters. However, the hepatic LDL-receptor mRNA levels were significantly low in the fish-oil-fed hamsters compared with the MIX-diet-fed hamsters. Increasing the amount of fish oil in the diet further decreased the hepatic LDL-receptor mRNA expression. It is concluded that F1B hamsters are susceptible to fish-oil-induced hyperlipidaemia, especially at high fat levels, and this increase is partially explained by the inhibition of hepatic LDL-receptor mRNA expression. SN - 0007-1145 UR - https://www.unboundmedicine.com/medline/citation/15005819/Hyperlipidaemic_effect_of_fish_oil_in_Bio_F1B_hamsters_ L2 - https://www.cambridge.org/core/product/identifier/S0007114504000467/type/journal_article DB - PRIME DP - Unbound Medicine ER -