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Effects of glutamine supplementation on innate immune response in rats with gut-derived sepsis.
Br J Nutr. 2004 Mar; 91(3):423-9.BJ

Abstract

The present study examined the effect of glutamine (Gln)-enriched diets before sepsis or Gln-containing total parenteral nutrition (TPN) after sepsis, or both, on the phagocytic activity and blood lymphocyte subpopulation in rats with gut-derived sepsis. Rats were assigned to a control group or one of four experimental groups. The control group and groups 1 and 2 were fed a semipurified diet; groups 3 and 4 had part of casein replaced by Gln. After feeding the diets for 10 d, sepsis was induced by caecal ligation and puncture (CLP); TPN was maintained for 3 d after CLP. The control group and groups 1 and 3 were infused with conventional TPN and groups 2 and 4 were supplemented with Gln in the TPN solution. All rats were killed 3 d after CLP or sham operation to examine their immune responses. The results showed that compared with the control group, the phagocytic activities of peritoneal macrophages were enhanced in groups 3 and 4, but not in groups 1 and 2. The proportion of CD3+ cells in group 1 was significantly lower (P<0.05) than that of the control group, whereas no differences were observed among the control and Gln-supplemented groups. The CD4+ cell proportion was significantly lower (P<0.05) in group 1 compared with the control group and groups 3 and 4. These findings suggest that Gln-enriched diets before CLP significantly enhanced peritoneal macrophage phagocytic activity, preserved CD4+ cells and maintained blood total T lymphocytes in gut-derived sepsis. However, parenteral Gln administration after caecal ligation and puncture had no favourable effects on modulating immune response in septic rats.

Authors+Show Affiliations

Institute of Nutrition and Health Sciences, Taipei Medical University, Taipei, Taiwan, Republic of China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15005828

Citation

Yeh, Sung-Ling, et al. "Effects of Glutamine Supplementation On Innate Immune Response in Rats With Gut-derived Sepsis." The British Journal of Nutrition, vol. 91, no. 3, 2004, pp. 423-9.
Yeh SL, Lai YN, Shang HF, et al. Effects of glutamine supplementation on innate immune response in rats with gut-derived sepsis. Br J Nutr. 2004;91(3):423-9.
Yeh, S. L., Lai, Y. N., Shang, H. F., Lin, M. T., & Chen, W. J. (2004). Effects of glutamine supplementation on innate immune response in rats with gut-derived sepsis. The British Journal of Nutrition, 91(3), 423-9.
Yeh SL, et al. Effects of Glutamine Supplementation On Innate Immune Response in Rats With Gut-derived Sepsis. Br J Nutr. 2004;91(3):423-9. PubMed PMID: 15005828.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effects of glutamine supplementation on innate immune response in rats with gut-derived sepsis. AU - Yeh,Sung-Ling, AU - Lai,Yu-Ni, AU - Shang,Huey-Fang, AU - Lin,Ming-Tsan, AU - Chen,Wei-Jao, PY - 2004/3/10/pubmed PY - 2004/3/31/medline PY - 2004/3/10/entrez SP - 423 EP - 9 JF - The British journal of nutrition JO - Br J Nutr VL - 91 IS - 3 N2 - The present study examined the effect of glutamine (Gln)-enriched diets before sepsis or Gln-containing total parenteral nutrition (TPN) after sepsis, or both, on the phagocytic activity and blood lymphocyte subpopulation in rats with gut-derived sepsis. Rats were assigned to a control group or one of four experimental groups. The control group and groups 1 and 2 were fed a semipurified diet; groups 3 and 4 had part of casein replaced by Gln. After feeding the diets for 10 d, sepsis was induced by caecal ligation and puncture (CLP); TPN was maintained for 3 d after CLP. The control group and groups 1 and 3 were infused with conventional TPN and groups 2 and 4 were supplemented with Gln in the TPN solution. All rats were killed 3 d after CLP or sham operation to examine their immune responses. The results showed that compared with the control group, the phagocytic activities of peritoneal macrophages were enhanced in groups 3 and 4, but not in groups 1 and 2. The proportion of CD3+ cells in group 1 was significantly lower (P<0.05) than that of the control group, whereas no differences were observed among the control and Gln-supplemented groups. The CD4+ cell proportion was significantly lower (P<0.05) in group 1 compared with the control group and groups 3 and 4. These findings suggest that Gln-enriched diets before CLP significantly enhanced peritoneal macrophage phagocytic activity, preserved CD4+ cells and maintained blood total T lymphocytes in gut-derived sepsis. However, parenteral Gln administration after caecal ligation and puncture had no favourable effects on modulating immune response in septic rats. SN - 0007-1145 UR - https://www.unboundmedicine.com/medline/citation/15005828/Effects_of_glutamine_supplementation_on_innate_immune_response_in_rats_with_gut_derived_sepsis_ L2 - https://www.cambridge.org/core/product/identifier/S0007114504000558/type/journal_article DB - PRIME DP - Unbound Medicine ER -