Tags

Type your tag names separated by a space and hit enter

Ischemic preconditioning attenuates ischemia-reperfusion-induced mucosal apoptosis by inhibiting the mitochondria-dependent pathway in rat small intestine.
Am J Physiol Gastrointest Liver Physiol. 2004 Apr; 286(4):G580-7.AJ

Abstract

Ischemic preconditioning provides a way of protecting organs from damage inflicted with prolonged ischemia-reperfusion. In this study, we investigated the mechanism of ischemic preconditioning involved in inhibition of prolonged ischemia-reperfusion-induced mucosal apoptosis in rat small intestine. Ischemic preconditioning was triggered by a transient occlusion of the superior mesenteric artery followed by reperfusion. Ischemia-reperfusion was induced by 60-min occlusion of the superior mesenteric artery followed by 60-min reperfusion in the small intestine. Ischemia-reperfusion alone induced mucosal apoptosis and mitochondrial respiratory dysfunction via promoted reactive oxygen species generation, reduced mitochondrial glutathione oxidation, increased mitochondrial lipid peroxidation, reduced mitochondrial membrane potential, and enhanced release of cytochrome c from mitochondria to activate caspase-9 and caspase-6 in the small intestine. Pretreatment with 20-min ischemia followed by 5-min reperfusion significantly inhibited the prolonged ischemia-reperfusion-induced mucosal apoptosis by 30%. Ischemic preconditioning ameliorated mitochondrial respiratory dysfunction by 50%, reduced reactive oxygen species generation by 38%, and suppressed mitochondrial lipid peroxidation by 36%, resulting in improvement of the mitochondrial membrane potential and prevention of cytochrome c release as well as caspase-6 activation. Results suggest that ischemic preconditioning attenuated ischemia-reperfusion-induced mucosal apoptosis partly by inhibiting the reactive oxygen species-mediated mitochondria-dependent pathway in the rat small intestine.

Authors+Show Affiliations

Department of Internal Medicine, Saga Medical School, Saga 849-8501, Japan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15010362

Citation

Wu, Bin, et al. "Ischemic Preconditioning Attenuates Ischemia-reperfusion-induced Mucosal Apoptosis By Inhibiting the Mitochondria-dependent Pathway in Rat Small Intestine." American Journal of Physiology. Gastrointestinal and Liver Physiology, vol. 286, no. 4, 2004, pp. G580-7.
Wu B, Ootani A, Iwakiri R, et al. Ischemic preconditioning attenuates ischemia-reperfusion-induced mucosal apoptosis by inhibiting the mitochondria-dependent pathway in rat small intestine. Am J Physiol Gastrointest Liver Physiol. 2004;286(4):G580-7.
Wu, B., Ootani, A., Iwakiri, R., Fujise, T., Tsunada, S., Toda, S., & Fujimoto, K. (2004). Ischemic preconditioning attenuates ischemia-reperfusion-induced mucosal apoptosis by inhibiting the mitochondria-dependent pathway in rat small intestine. American Journal of Physiology. Gastrointestinal and Liver Physiology, 286(4), G580-7.
Wu B, et al. Ischemic Preconditioning Attenuates Ischemia-reperfusion-induced Mucosal Apoptosis By Inhibiting the Mitochondria-dependent Pathway in Rat Small Intestine. Am J Physiol Gastrointest Liver Physiol. 2004;286(4):G580-7. PubMed PMID: 15010362.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Ischemic preconditioning attenuates ischemia-reperfusion-induced mucosal apoptosis by inhibiting the mitochondria-dependent pathway in rat small intestine. AU - Wu,Bin, AU - Ootani,Akifumi, AU - Iwakiri,Ryuichi, AU - Fujise,Takehiro, AU - Tsunada,Seiji, AU - Toda,Shuji, AU - Fujimoto,Kazuma, PY - 2004/3/11/pubmed PY - 2004/4/20/medline PY - 2004/3/11/entrez SP - G580 EP - 7 JF - American journal of physiology. Gastrointestinal and liver physiology JO - Am J Physiol Gastrointest Liver Physiol VL - 286 IS - 4 N2 - Ischemic preconditioning provides a way of protecting organs from damage inflicted with prolonged ischemia-reperfusion. In this study, we investigated the mechanism of ischemic preconditioning involved in inhibition of prolonged ischemia-reperfusion-induced mucosal apoptosis in rat small intestine. Ischemic preconditioning was triggered by a transient occlusion of the superior mesenteric artery followed by reperfusion. Ischemia-reperfusion was induced by 60-min occlusion of the superior mesenteric artery followed by 60-min reperfusion in the small intestine. Ischemia-reperfusion alone induced mucosal apoptosis and mitochondrial respiratory dysfunction via promoted reactive oxygen species generation, reduced mitochondrial glutathione oxidation, increased mitochondrial lipid peroxidation, reduced mitochondrial membrane potential, and enhanced release of cytochrome c from mitochondria to activate caspase-9 and caspase-6 in the small intestine. Pretreatment with 20-min ischemia followed by 5-min reperfusion significantly inhibited the prolonged ischemia-reperfusion-induced mucosal apoptosis by 30%. Ischemic preconditioning ameliorated mitochondrial respiratory dysfunction by 50%, reduced reactive oxygen species generation by 38%, and suppressed mitochondrial lipid peroxidation by 36%, resulting in improvement of the mitochondrial membrane potential and prevention of cytochrome c release as well as caspase-6 activation. Results suggest that ischemic preconditioning attenuated ischemia-reperfusion-induced mucosal apoptosis partly by inhibiting the reactive oxygen species-mediated mitochondria-dependent pathway in the rat small intestine. SN - 0193-1857 UR - https://www.unboundmedicine.com/medline/citation/15010362/Ischemic_preconditioning_attenuates_ischemia_reperfusion_induced_mucosal_apoptosis_by_inhibiting_the_mitochondria_dependent_pathway_in_rat_small_intestine_ L2 - https://journals.physiology.org/doi/10.1152/ajpgi.00335.2003?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -