Tags

Type your tag names separated by a space and hit enter

Relationship between copper- and zinc-induced oxidative stress and proline accumulation in Scenedesmus sp.
Planta. 2004 Jul; 219(3):397-404.P

Abstract

A 4-h exposure of Scenedesmus sp. to Cu or Zn enhanced intracellular levels of both test metals and proline. The level of intracellular proline increased markedly up to 10 microM Cu, but higher concentrations were inhibitory. However, intracellular proline consistently increased with increasing concentration of Zn in the medium. Cu and Zn induced oxidative stress in the test alga by increasing lipid peroxidation and membrane permeability, and by reducing SH content. Pretreatment of the test alga with 1 mM proline for 30 min completely alleviated Cu-induced lipid peroxidation, minimized K+ efflux and also reduced depletion of the SH pool. But proline pretreatment could only slightly reduce Zn-induced oxidative stress. Interestingly, proline pretreatment increased the level of Cu (25-54%) and Zn (19-49%) inside the cells. It did not affect the activities of superoxide dismutase, ascorbate peroxidase or catalase, but improved glutathione reductase activity under Cu and Zn stress. A comparison of the effects of proline pretreatment on lipid peroxidation by Cu, Zn, methyl viologen and ultraviolet-B radiation suggests that proline protects cells from metal-induced oxidative stress by scavenging reactive oxygen species rather than by chelating metal ions. Pretreatment of cells with a known antioxidant (ascorbate) and a hydroxyl radical scavenger (sodium benzoate) considerably reduced metal-induced lipid peroxidation and proline accumulation. However, sodium benzoate had a very mild effect on Zn-induced lipid peroxidation and proline accumulation. The present study demonstrates that proline possibly acts by detoxifying reactive oxygen species, mainly hydroxyl radicals, rather than by improving the antioxidant defense system under metal stress.

Authors+Show Affiliations

Tripathi BN
Laboratory of Algal Biology, Department of Botany, Banaras Hindu University, 221005, Varanasi, India.
Gaur JP
No affiliation info available

MeSH

Ascorbate PeroxidasesCatalaseCopperFree Radical ScavengersGlutathione ReductaseLipid PeroxidationOxidative StressPeroxidasesProlineScenedesmusSuperoxide DismutaseZinc

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15014994

Citation

Tripathi, B N., and J P. Gaur. "Relationship Between Copper- and Zinc-induced Oxidative Stress and Proline Accumulation in Scenedesmus Sp." Planta, vol. 219, no. 3, 2004, pp. 397-404.
Tripathi BN, Gaur JP. Relationship between copper- and zinc-induced oxidative stress and proline accumulation in Scenedesmus sp. Planta. 2004;219(3):397-404.
Tripathi, B. N., & Gaur, J. P. (2004). Relationship between copper- and zinc-induced oxidative stress and proline accumulation in Scenedesmus sp. Planta, 219(3), 397-404.
Tripathi BN, Gaur JP. Relationship Between Copper- and Zinc-induced Oxidative Stress and Proline Accumulation in Scenedesmus Sp. Planta. 2004;219(3):397-404. PubMed PMID: 15014994.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Relationship between copper- and zinc-induced oxidative stress and proline accumulation in Scenedesmus sp. AU - Tripathi,B N, AU - Gaur,J P, Y1 - 2004/03/11/ PY - 2003/11/03/received PY - 2004/02/05/accepted PY - 2004/3/12/pubmed PY - 2004/10/6/medline PY - 2004/3/12/entrez SP - 397 EP - 404 JF - Planta JO - Planta VL - 219 IS - 3 N2 - A 4-h exposure of Scenedesmus sp. to Cu or Zn enhanced intracellular levels of both test metals and proline. The level of intracellular proline increased markedly up to 10 microM Cu, but higher concentrations were inhibitory. However, intracellular proline consistently increased with increasing concentration of Zn in the medium. Cu and Zn induced oxidative stress in the test alga by increasing lipid peroxidation and membrane permeability, and by reducing SH content. Pretreatment of the test alga with 1 mM proline for 30 min completely alleviated Cu-induced lipid peroxidation, minimized K+ efflux and also reduced depletion of the SH pool. But proline pretreatment could only slightly reduce Zn-induced oxidative stress. Interestingly, proline pretreatment increased the level of Cu (25-54%) and Zn (19-49%) inside the cells. It did not affect the activities of superoxide dismutase, ascorbate peroxidase or catalase, but improved glutathione reductase activity under Cu and Zn stress. A comparison of the effects of proline pretreatment on lipid peroxidation by Cu, Zn, methyl viologen and ultraviolet-B radiation suggests that proline protects cells from metal-induced oxidative stress by scavenging reactive oxygen species rather than by chelating metal ions. Pretreatment of cells with a known antioxidant (ascorbate) and a hydroxyl radical scavenger (sodium benzoate) considerably reduced metal-induced lipid peroxidation and proline accumulation. However, sodium benzoate had a very mild effect on Zn-induced lipid peroxidation and proline accumulation. The present study demonstrates that proline possibly acts by detoxifying reactive oxygen species, mainly hydroxyl radicals, rather than by improving the antioxidant defense system under metal stress. SN - 0032-0935 UR - https://www.unboundmedicine.com/medline/citation/15014994/Relationship_between_copper__and_zinc_induced_oxidative_stress_and_proline_accumulation_in_Scenedesmus_sp_ DB - PRIME DP - Unbound Medicine ER -