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[Determination of puerarin, daidzin and daidzein in root of Pueraria lobata of different origin by HPLC].
Zhongguo Zhong Yao Za Zhi. 2003 Jan; 28(1):49-51.ZZ

Abstract

OBJECTIVE

To develop an HPLC method to determine Puerarin, Daidzin and Daidzein in Pueraria lobata.

METHOD

The separation was performed in a SYMMETRY C18 column with a mobile phase of methanol-1% HAC solution (gradient elution), the The detection wavelength being 250 nm.

RESULT

The average recovery was respectively 101.7%, 100.7% and 101.7% (n = 3), RSD 0.43%, 0.82% and 1.50% (n = 3) for Puerarin, Daidzin and Daidzein.

CONCLUSION

This method is suitable for the determination of Puerarin, Daidzin and Daidzein in Pueraria lobata and its preparation.

Authors+Show Affiliations

Beijing Yuke Botanical Development CO., LTD., Beijing 100062, China. jinwenshan@yahoo.com.cnNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

English Abstract
Journal Article

Language

chi

PubMed ID

15015267

Citation

Jin, Wen-shan, et al. "[Determination of Puerarin, Daidzin and Daidzein in Root of Pueraria Lobata of Different Origin By HPLC]." Zhongguo Zhong Yao Za Zhi = Zhongguo Zhongyao Zazhi = China Journal of Chinese Materia Medica, vol. 28, no. 1, 2003, pp. 49-51.
Jin WS, Tan YY, Chen YG, et al. [Determination of puerarin, daidzin and daidzein in root of Pueraria lobata of different origin by HPLC]. Zhongguo Zhong Yao Za Zhi. 2003;28(1):49-51.
Jin, W. S., Tan, Y. Y., Chen, Y. G., & Wang, Y. (2003). [Determination of puerarin, daidzin and daidzein in root of Pueraria lobata of different origin by HPLC]. Zhongguo Zhong Yao Za Zhi = Zhongguo Zhongyao Zazhi = China Journal of Chinese Materia Medica, 28(1), 49-51.
Jin WS, et al. [Determination of Puerarin, Daidzin and Daidzein in Root of Pueraria Lobata of Different Origin By HPLC]. Zhongguo Zhong Yao Za Zhi. 2003;28(1):49-51. PubMed PMID: 15015267.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Determination of puerarin, daidzin and daidzein in root of Pueraria lobata of different origin by HPLC]. AU - Jin,Wen-shan, AU - Tan,Yu-yuan, AU - Chen,You-gen, AU - Wang,Yan, PY - 2004/3/16/pubmed PY - 2004/7/16/medline PY - 2004/3/16/entrez SP - 49 EP - 51 JF - Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica JO - Zhongguo Zhong Yao Za Zhi VL - 28 IS - 1 N2 - OBJECTIVE: To develop an HPLC method to determine Puerarin, Daidzin and Daidzein in Pueraria lobata. METHOD: The separation was performed in a SYMMETRY C18 column with a mobile phase of methanol-1% HAC solution (gradient elution), the The detection wavelength being 250 nm. RESULT: The average recovery was respectively 101.7%, 100.7% and 101.7% (n = 3), RSD 0.43%, 0.82% and 1.50% (n = 3) for Puerarin, Daidzin and Daidzein. CONCLUSION: This method is suitable for the determination of Puerarin, Daidzin and Daidzein in Pueraria lobata and its preparation. SN - 1001-5302 UR - https://www.unboundmedicine.com/medline/citation/15015267/[Determination_of_puerarin_daidzin_and_daidzein_in_root_of_Pueraria_lobata_of_different_origin_by_HPLC]_ DB - PRIME DP - Unbound Medicine ER -