Tags

Type your tag names separated by a space and hit enter

Reverse microdialysis of a dopamine D2 receptor antagonist alters extracellular adenosine levels in the rat nucleus accumbens.
Neurochem Int. 2004 Jun; 44(8):609-15.NI

Abstract

Recent evidence suggests that modulation of dopaminergic transmission alters striatal levels of extracellular adenosine. The present study used reverse microdialysis of the selective dopamine D(2) receptor antagonist raclopride to investigate whether a blockade of dopamine D(2) receptors modifies extracellular adenosine concentrations in the nucleus accumbens. Results reveal that perfusion of raclopride produced an increase of dialysate adenosine which was significant with a high (10 mM) and intermediate (1 mM) drug concentration, but not with lower drug concentrations (10 and 100 microM). Thus, the present study demonstrates that a selective blockade of dopamine D(2) receptors in the nucleus accumbens produced a pronounced increase of extracellular adenosine. The cellular mechanisms underlying this effect are yet unknown. It is suggested that the increase of extracellular adenosine might be related to a homeostatic modulatory mechanism proposed to be a key function of adenosine in response to neuronal metabolic challenges.

Authors+Show Affiliations

Department of Animal Physiology, Institute of Biology, University of Stuttgart, Pfaffenwaldring 57, Stuttgart D-70550, Germany.No affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15016476

Citation

Nagel, J, and W Hauber. "Reverse Microdialysis of a Dopamine D2 Receptor Antagonist Alters Extracellular Adenosine Levels in the Rat Nucleus Accumbens." Neurochemistry International, vol. 44, no. 8, 2004, pp. 609-15.
Nagel J, Hauber W. Reverse microdialysis of a dopamine D2 receptor antagonist alters extracellular adenosine levels in the rat nucleus accumbens. Neurochem Int. 2004;44(8):609-15.
Nagel, J., & Hauber, W. (2004). Reverse microdialysis of a dopamine D2 receptor antagonist alters extracellular adenosine levels in the rat nucleus accumbens. Neurochemistry International, 44(8), 609-15.
Nagel J, Hauber W. Reverse Microdialysis of a Dopamine D2 Receptor Antagonist Alters Extracellular Adenosine Levels in the Rat Nucleus Accumbens. Neurochem Int. 2004;44(8):609-15. PubMed PMID: 15016476.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Reverse microdialysis of a dopamine D2 receptor antagonist alters extracellular adenosine levels in the rat nucleus accumbens. AU - Nagel,J, AU - Hauber,W, PY - 2003/03/17/received PY - 2003/10/02/accepted PY - 2004/3/16/pubmed PY - 2004/5/8/medline PY - 2004/3/16/entrez SP - 609 EP - 15 JF - Neurochemistry international JO - Neurochem Int VL - 44 IS - 8 N2 - Recent evidence suggests that modulation of dopaminergic transmission alters striatal levels of extracellular adenosine. The present study used reverse microdialysis of the selective dopamine D(2) receptor antagonist raclopride to investigate whether a blockade of dopamine D(2) receptors modifies extracellular adenosine concentrations in the nucleus accumbens. Results reveal that perfusion of raclopride produced an increase of dialysate adenosine which was significant with a high (10 mM) and intermediate (1 mM) drug concentration, but not with lower drug concentrations (10 and 100 microM). Thus, the present study demonstrates that a selective blockade of dopamine D(2) receptors in the nucleus accumbens produced a pronounced increase of extracellular adenosine. The cellular mechanisms underlying this effect are yet unknown. It is suggested that the increase of extracellular adenosine might be related to a homeostatic modulatory mechanism proposed to be a key function of adenosine in response to neuronal metabolic challenges. SN - 0197-0186 UR - https://www.unboundmedicine.com/medline/citation/15016476/Reverse_microdialysis_of_a_dopamine_D2_receptor_antagonist_alters_extracellular_adenosine_levels_in_the_rat_nucleus_accumbens_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0197018603002225 DB - PRIME DP - Unbound Medicine ER -