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Immunosensor for detection of Yersinia enterocolitica based on imaging ellipsometry.
Anal Chem. 2004 Mar 15; 76(6):1799-803.AC

Abstract

An immunosensor for the detection of pathogens was developed using imaging ellipsometry (IE) as a detection method. Yersinia enterocolitica was selected as the target pathogen in this study. A gold surface deposited with a self-assembled layer of 11-mercaptoundecanoic acid (11-MUA) was used as a substrate. For the fabrication of the immunosensor, protein G spots were made on the substrate using an inkjet-type microarrayer, and monoclonal antibody (Mab) was adsorbed onto the protein G spots. Deposition of each layer onto the substrate was confirmed by the measurement of surface plasmon resonance. The ellipsometric image of the protein G spot and the Mab-adsorbed protein G spot were acquired using an off-null ellipsometry type of imaging ellipsometry system. By measuring the ellipsometric angles of the protein layers, the surface concentration of each protein layer was calculated. The change in the mean optical intensity of the protein spot to the various concentrations of Y.enterocolitica was estimated. The immunosensor using imaging ellipsometry could successfully detect Y. enterocolitica in concentrations varying from 10(3) to 10(7) cfu/mL. The proposed immunosensor system has the advantage of allowing label-free detection, high sensitivity, and operational simplicity.

Authors+Show Affiliations

Bae YM
Department of Chemical and Biomolecular Engineering, Sogang University, 1 Sinsu-Dong, Mapo-Gu, Seoul 121-742, Korea.
Oh BK
No affiliation info available
Lee W
No affiliation info available
Lee WH
No affiliation info available
Choi JW
No affiliation info available

MeSH

Antibody AffinityBacterial ProteinsBiosensing TechniquesImage Processing, Computer-AssistedImmobilizationImmunoassayRecombinant ProteinsSurface PropertiesYersinia InfectionsYersinia enterocolitica

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15018586

Citation

Bae, Young Min, et al. "Immunosensor for Detection of Yersinia Enterocolitica Based On Imaging Ellipsometry." Analytical Chemistry, vol. 76, no. 6, 2004, pp. 1799-803.
Bae YM, Oh BK, Lee W, et al. Immunosensor for detection of Yersinia enterocolitica based on imaging ellipsometry. Anal Chem. 2004;76(6):1799-803.
Bae, Y. M., Oh, B. K., Lee, W., Lee, W. H., & Choi, J. W. (2004). Immunosensor for detection of Yersinia enterocolitica based on imaging ellipsometry. Analytical Chemistry, 76(6), 1799-803.
Bae YM, et al. Immunosensor for Detection of Yersinia Enterocolitica Based On Imaging Ellipsometry. Anal Chem. 2004 Mar 15;76(6):1799-803. PubMed PMID: 15018586.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Immunosensor for detection of Yersinia enterocolitica based on imaging ellipsometry. AU - Bae,Young Min, AU - Oh,Byung-Keun, AU - Lee,Woochang, AU - Lee,Won Hong, AU - Choi,Jeong-Woo, PY - 2004/3/17/pubmed PY - 2004/12/16/medline PY - 2004/3/17/entrez SP - 1799 EP - 803 JF - Analytical chemistry JO - Anal Chem VL - 76 IS - 6 N2 - An immunosensor for the detection of pathogens was developed using imaging ellipsometry (IE) as a detection method. Yersinia enterocolitica was selected as the target pathogen in this study. A gold surface deposited with a self-assembled layer of 11-mercaptoundecanoic acid (11-MUA) was used as a substrate. For the fabrication of the immunosensor, protein G spots were made on the substrate using an inkjet-type microarrayer, and monoclonal antibody (Mab) was adsorbed onto the protein G spots. Deposition of each layer onto the substrate was confirmed by the measurement of surface plasmon resonance. The ellipsometric image of the protein G spot and the Mab-adsorbed protein G spot were acquired using an off-null ellipsometry type of imaging ellipsometry system. By measuring the ellipsometric angles of the protein layers, the surface concentration of each protein layer was calculated. The change in the mean optical intensity of the protein spot to the various concentrations of Y.enterocolitica was estimated. The immunosensor using imaging ellipsometry could successfully detect Y. enterocolitica in concentrations varying from 10(3) to 10(7) cfu/mL. The proposed immunosensor system has the advantage of allowing label-free detection, high sensitivity, and operational simplicity. SN - 0003-2700 UR - https://www.unboundmedicine.com/medline/citation/15018586/Immunosensor_for_detection_of_Yersinia_enterocolitica_based_on_imaging_ellipsometry_ DB - PRIME DP - Unbound Medicine ER -