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Determination of nicotine and cotinine in tobacco harvesters' urine by solid-phase extraction and liquid chromatography.
J Chromatogr B Analyt Technol Biomed Life Sci. 2004 Apr 05; 802(2):323-8.JC

Abstract

A solid-phase extraction method using Drug Test-1 column containing chemically modified silica as a solid support for sample clean up and reversed phase ion-paired high-pressure liquid chromatography method have been developed for the simultaneous determination of nicotine and its metabolite cotinine from the urine samples. Mobile phase was consisted of acetate buffer (containing 0.03 M sodium acetate and 0.1 M acetic acid) pH 3.1 and acetonitrile (78:22% (v/v)) containing 0.02 M sodium octanosulfonate as an ion pair agent. pH of the mobile phase was adjusted to 3.6 with triethylamine for better resolution and to prevent peak tailing. The linearity was obtained in the range of 0.5-10 microg/ml concentrations of nicotine and cotinine standards. The correlation coefficients were 0.998 for cotinine and 0.999 for nicotine. The recoveries were obtained in the range of 79-97% with average value of 85% for nicotine and in the range of 82-98% with average value of 88% for cotinine. The limit of detection was 2 ng/ml for cotinine and 5 ng/ml for nicotine with 2 ml urine for extraction, calculated by taking signal to noise ratio 10:3. The intra-day co-efficient of variation (CV) were <4 and 7% and inter-day CV were <9 and 7% for nicotine and cotinine, respectively. The method was applied to the urine samples of tobacco harvesters, who suffer from green tobacco sickness (GTS) to check the absorption of nicotine through dermal route during the various processes of tobacco cultivation due to its good reproducibility and sensitivity.

Authors+Show Affiliations

Doctor PB
National Institute of Occupational Health, Meghani Nagar, Ahmedabad, Gujarat 380016, India. pbdoctor@rediffmail.com
Gokani VN
No affiliation info available
Kulkarni PK
No affiliation info available
Parikh JR
No affiliation info available
Saiyed HN
No affiliation info available

MeSH

Chromatography, High Pressure LiquidCotinineHumansNicotineOccupationsReference StandardsReproducibility of ResultsSensitivity and Specificity

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15018794

Citation

Doctor, P B., et al. "Determination of Nicotine and Cotinine in Tobacco Harvesters' Urine By Solid-phase Extraction and Liquid Chromatography." Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences, vol. 802, no. 2, 2004, pp. 323-8.
Doctor PB, Gokani VN, Kulkarni PK, et al. Determination of nicotine and cotinine in tobacco harvesters' urine by solid-phase extraction and liquid chromatography. J Chromatogr B Analyt Technol Biomed Life Sci. 2004;802(2):323-8.
Doctor, P. B., Gokani, V. N., Kulkarni, P. K., Parikh, J. R., & Saiyed, H. N. (2004). Determination of nicotine and cotinine in tobacco harvesters' urine by solid-phase extraction and liquid chromatography. Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences, 802(2), 323-8.
Doctor PB, et al. Determination of Nicotine and Cotinine in Tobacco Harvesters' Urine By Solid-phase Extraction and Liquid Chromatography. J Chromatogr B Analyt Technol Biomed Life Sci. 2004 Apr 5;802(2):323-8. PubMed PMID: 15018794.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Determination of nicotine and cotinine in tobacco harvesters' urine by solid-phase extraction and liquid chromatography. AU - Doctor,P B, AU - Gokani,V N, AU - Kulkarni,P K, AU - Parikh,J R, AU - Saiyed,H N, PY - 2003/04/22/received PY - 2003/12/02/revised PY - 2003/12/16/accepted PY - 2004/3/17/pubmed PY - 2004/12/16/medline PY - 2004/3/17/entrez SP - 323 EP - 8 JF - Journal of chromatography. B, Analytical technologies in the biomedical and life sciences JO - J Chromatogr B Analyt Technol Biomed Life Sci VL - 802 IS - 2 N2 - A solid-phase extraction method using Drug Test-1 column containing chemically modified silica as a solid support for sample clean up and reversed phase ion-paired high-pressure liquid chromatography method have been developed for the simultaneous determination of nicotine and its metabolite cotinine from the urine samples. Mobile phase was consisted of acetate buffer (containing 0.03 M sodium acetate and 0.1 M acetic acid) pH 3.1 and acetonitrile (78:22% (v/v)) containing 0.02 M sodium octanosulfonate as an ion pair agent. pH of the mobile phase was adjusted to 3.6 with triethylamine for better resolution and to prevent peak tailing. The linearity was obtained in the range of 0.5-10 microg/ml concentrations of nicotine and cotinine standards. The correlation coefficients were 0.998 for cotinine and 0.999 for nicotine. The recoveries were obtained in the range of 79-97% with average value of 85% for nicotine and in the range of 82-98% with average value of 88% for cotinine. The limit of detection was 2 ng/ml for cotinine and 5 ng/ml for nicotine with 2 ml urine for extraction, calculated by taking signal to noise ratio 10:3. The intra-day co-efficient of variation (CV) were <4 and 7% and inter-day CV were <9 and 7% for nicotine and cotinine, respectively. The method was applied to the urine samples of tobacco harvesters, who suffer from green tobacco sickness (GTS) to check the absorption of nicotine through dermal route during the various processes of tobacco cultivation due to its good reproducibility and sensitivity. SN - 1570-0232 UR - https://www.unboundmedicine.com/medline/citation/15018794/Determination_of_nicotine_and_cotinine_in_tobacco_harvesters'_urine_by_solid_phase_extraction_and_liquid_chromatography_ DB - PRIME DP - Unbound Medicine ER -