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Accurate determination of oosporein in fungal culture broth by differential pulse polarography.
J Agric Food Chem. 2004 Mar 24; 52(6):1423-6.JA

Abstract

A simple and accurate differential pulse polarographic method has been developed for the determination of oosporein in the culture broth of the fungus Beauveria brongniartii. This hydroxybenzoquinone derivative is the only major secondary metabolite secreted by this entomopathogenic fungus, which is used as biological pest control agent (BCA) against Melolontha melolontha larvae. It can be found in the host organism as well as in the formulated product. The polarographic behavior of oosporein was examined in various buffer systems over the pH range 3-10. In Britton-Robinson buffer/methanol solution (3:7 v/v, pH 5.5) the differential pulse polarograms exhibited reproducible peaks at E(p) = -0.18 V vs silver/silver chloride/potassium chloride (3 M). Under these conditions, a plot of peak height vs concentration of oosporein was found to be linear over the range 5.9 x 10(-)(7) to 2.5 x 10(-)(5) M (0.18-7.74 microg mL(-)(1); r = 0.9998). The detection limit was calculated to be 54 ng mL(-)(1). To evaluate the concentration of oosporein, the standard addition method was applied. The analysis of oosporein in the culture broth led to a mean value of 524.9 microg mL(-)(1) broth with a relative standard deviation (S(rel)) of +/-2.6%. The proposed polarographic method is accurate, not time-consuming, and it is of low cost because no separation steps are necessary.

Authors+Show Affiliations

Institute of Pharmaceutical Chemistry and Pharmaceutical Technology, Karl-Franzens-University, A-8010 Graz, Austria. astrid.ortner@kfunigraz.ac.atNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15030190

Citation

Michelitsch, Astrid, et al. "Accurate Determination of Oosporein in Fungal Culture Broth By Differential Pulse Polarography." Journal of Agricultural and Food Chemistry, vol. 52, no. 6, 2004, pp. 1423-6.
Michelitsch A, Rückert U, Rittmannsberger A, et al. Accurate determination of oosporein in fungal culture broth by differential pulse polarography. J Agric Food Chem. 2004;52(6):1423-6.
Michelitsch, A., Rückert, U., Rittmannsberger, A., Seger, C., Strasser, H., & Likussar, W. (2004). Accurate determination of oosporein in fungal culture broth by differential pulse polarography. Journal of Agricultural and Food Chemistry, 52(6), 1423-6.
Michelitsch A, et al. Accurate Determination of Oosporein in Fungal Culture Broth By Differential Pulse Polarography. J Agric Food Chem. 2004 Mar 24;52(6):1423-6. PubMed PMID: 15030190.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Accurate determination of oosporein in fungal culture broth by differential pulse polarography. AU - Michelitsch,Astrid, AU - Rückert,Ulla, AU - Rittmannsberger,Anna, AU - Seger,Christoph, AU - Strasser,Hermann, AU - Likussar,Werner, PY - 2004/3/20/pubmed PY - 2004/5/8/medline PY - 2004/3/20/entrez SP - 1423 EP - 6 JF - Journal of agricultural and food chemistry JO - J Agric Food Chem VL - 52 IS - 6 N2 - A simple and accurate differential pulse polarographic method has been developed for the determination of oosporein in the culture broth of the fungus Beauveria brongniartii. This hydroxybenzoquinone derivative is the only major secondary metabolite secreted by this entomopathogenic fungus, which is used as biological pest control agent (BCA) against Melolontha melolontha larvae. It can be found in the host organism as well as in the formulated product. The polarographic behavior of oosporein was examined in various buffer systems over the pH range 3-10. In Britton-Robinson buffer/methanol solution (3:7 v/v, pH 5.5) the differential pulse polarograms exhibited reproducible peaks at E(p) = -0.18 V vs silver/silver chloride/potassium chloride (3 M). Under these conditions, a plot of peak height vs concentration of oosporein was found to be linear over the range 5.9 x 10(-)(7) to 2.5 x 10(-)(5) M (0.18-7.74 microg mL(-)(1); r = 0.9998). The detection limit was calculated to be 54 ng mL(-)(1). To evaluate the concentration of oosporein, the standard addition method was applied. The analysis of oosporein in the culture broth led to a mean value of 524.9 microg mL(-)(1) broth with a relative standard deviation (S(rel)) of +/-2.6%. The proposed polarographic method is accurate, not time-consuming, and it is of low cost because no separation steps are necessary. SN - 0021-8561 UR - https://www.unboundmedicine.com/medline/citation/15030190/Accurate_determination_of_oosporein_in_fungal_culture_broth_by_differential_pulse_polarography_ L2 - https://doi.org/10.1021/jf0307494 DB - PRIME DP - Unbound Medicine ER -