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Bcl-x L blocks mitochondrial multiple conductance channel activation and inhibits 6-OHDA-induced death in SH-SY5Y cells.
J Neurochem. 2004 Apr; 89(1):124-33.JN

Abstract

Apoptosis is an active process that is regulated by different signalling pathways. One of the more important organelles involved in apoptosis regulation is the mitochondrion. Electron chain transport disruption increases free radical production leading to multiple conductance channel opening, release of cytochrome c and caspase activation. This death pathway can be blocked by anti-apoptotic members of the Bcl-2 protein family that might shift redox potential to a more reduced state, preventing free radical-mediated damage. 6-Hydroxydopamine (6-OHDA) has been widely used to generate Parkinson's disease-like models. It is able to generate free radicals and to induce catecholaminergic cell death. In this paper we have used the human neuroblastoma cell line SH-SY5Y overexpressing Bcl-x(L) as a model to gain insights into the mechanisms through which Bcl-x(L) blocks 6-OHDA-induced cell death and to identify the molecular targets for this action. Herein, we present evidence supporting that the Bcl-x(L)-anti-apoptotic signal pathway seems to prevent mitochondrial multiple conductance channel opening, cytochrome c release and caspase-3 like activity following 6-OHDA treatment in the human neuroblastoma cell line SH-SY5Y.

Authors+Show Affiliations

Centro Regional de Investigaciones Biomédicas, Universidad de Castilla-La Mancha, Albacete, Spain.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15030396

Citation

Jordán, Joaquín, et al. "Bcl-x L Blocks Mitochondrial Multiple Conductance Channel Activation and Inhibits 6-OHDA-induced Death in SH-SY5Y Cells." Journal of Neurochemistry, vol. 89, no. 1, 2004, pp. 124-33.
Jordán J, Galindo MF, Tornero D, et al. Bcl-x L blocks mitochondrial multiple conductance channel activation and inhibits 6-OHDA-induced death in SH-SY5Y cells. J Neurochem. 2004;89(1):124-33.
Jordán, J., Galindo, M. F., Tornero, D., González-García, C., & Ceña, V. (2004). Bcl-x L blocks mitochondrial multiple conductance channel activation and inhibits 6-OHDA-induced death in SH-SY5Y cells. Journal of Neurochemistry, 89(1), 124-33.
Jordán J, et al. Bcl-x L Blocks Mitochondrial Multiple Conductance Channel Activation and Inhibits 6-OHDA-induced Death in SH-SY5Y Cells. J Neurochem. 2004;89(1):124-33. PubMed PMID: 15030396.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Bcl-x L blocks mitochondrial multiple conductance channel activation and inhibits 6-OHDA-induced death in SH-SY5Y cells. AU - Jordán,Joaquín, AU - Galindo,María F, AU - Tornero,Daniel, AU - González-García,Carmen, AU - Ceña,Valentín, PY - 2004/3/20/pubmed PY - 2004/4/27/medline PY - 2004/3/20/entrez SP - 124 EP - 33 JF - Journal of neurochemistry JO - J Neurochem VL - 89 IS - 1 N2 - Apoptosis is an active process that is regulated by different signalling pathways. One of the more important organelles involved in apoptosis regulation is the mitochondrion. Electron chain transport disruption increases free radical production leading to multiple conductance channel opening, release of cytochrome c and caspase activation. This death pathway can be blocked by anti-apoptotic members of the Bcl-2 protein family that might shift redox potential to a more reduced state, preventing free radical-mediated damage. 6-Hydroxydopamine (6-OHDA) has been widely used to generate Parkinson's disease-like models. It is able to generate free radicals and to induce catecholaminergic cell death. In this paper we have used the human neuroblastoma cell line SH-SY5Y overexpressing Bcl-x(L) as a model to gain insights into the mechanisms through which Bcl-x(L) blocks 6-OHDA-induced cell death and to identify the molecular targets for this action. Herein, we present evidence supporting that the Bcl-x(L)-anti-apoptotic signal pathway seems to prevent mitochondrial multiple conductance channel opening, cytochrome c release and caspase-3 like activity following 6-OHDA treatment in the human neuroblastoma cell line SH-SY5Y. SN - 0022-3042 UR - https://www.unboundmedicine.com/medline/citation/15030396/Bcl_x_L_blocks_mitochondrial_multiple_conductance_channel_activation_and_inhibits_6_OHDA_induced_death_in_SH_SY5Y_cells_ L2 - https://onlinelibrary.wiley.com/resolve/openurl?genre=article&sid=nlm:pubmed&issn=0022-3042&date=2004&volume=89&issue=1&spage=124 DB - PRIME DP - Unbound Medicine ER -