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Spill-over of European bat lyssavirus type 1 into a stone marten (Martes foina) in Germany.

Abstract

European bat lyssavirus type 1 (EBLV-1, genotype 5) is known to endemically circulate in insectivorous bat populations in Germany. In August 2001, a rabies suspect stone marten (Martes foina) was found in the city of Burg (Saxony-Anhalt, Germany) and was sent to the regional veterinary laboratory for routine rabies diagnosis. Whereas brain samples repeatedly tested negative in the fluorescent antibody test for classical rabies virus (genotype 1), the mouse inoculation test and the rabies tissue culture inoculation test yielded positive results. Rabies viral RNA was also detected in the stone marten brain sample both by nested and heminested RT-PCR specific for the nucleoprotein gene and for the nucleoprotein phosphoprotein junction of rabies virus. The amplification products were sequenced to genotype the isolate. Sequence data obtained from the first-round RT-PCR products were analysed and the suspect stone marten isolate was confirmed as a rabies related virus (EBLV-1a). Phylogenetic comparison with sequences from recent genotype five isolates from Germany and Denmark showed that it was closely related to a previous isolate of EBLV-1 from a serotine bat in Saxony-Anhalt obtained in the same year in an area adjacent to the place where the EBLV-1 infected stone marten was found. Both EBLV-1 isolates share a 99.5% identity. This is the first report of an EBLV-1a spill-over from an insectivorous bat into wildlife in Europe.

Authors+Show Affiliations

WHO Collaborating Centre for Rabies Surveillance and Research, Institute of Epidemiology, Federal Research Centre for Virus Diseases of Animals, 16868 Wusterhausen, Germany. Thomas.Mueller@wus.bfav.deNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Case Reports
Journal Article

Language

eng

PubMed ID

15030600

Citation

Müller, T, et al. "Spill-over of European Bat Lyssavirus Type 1 Into a Stone Marten (Martes Foina) in Germany." Journal of Veterinary Medicine. B, Infectious Diseases and Veterinary Public Health, vol. 51, no. 2, 2004, pp. 49-54.
Müller T, Cox J, Peter W, et al. Spill-over of European bat lyssavirus type 1 into a stone marten (Martes foina) in Germany. J Vet Med B Infect Dis Vet Public Health. 2004;51(2):49-54.
Müller, T., Cox, J., Peter, W., Schäfer, R., Johnson, N., McElhinney, L. M., Geue, J. L., Tjørnehøj, K., & Fooks, A. R. (2004). Spill-over of European bat lyssavirus type 1 into a stone marten (Martes foina) in Germany. Journal of Veterinary Medicine. B, Infectious Diseases and Veterinary Public Health, 51(2), 49-54.
Müller T, et al. Spill-over of European Bat Lyssavirus Type 1 Into a Stone Marten (Martes Foina) in Germany. J Vet Med B Infect Dis Vet Public Health. 2004;51(2):49-54. PubMed PMID: 15030600.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Spill-over of European bat lyssavirus type 1 into a stone marten (Martes foina) in Germany. AU - Müller,T, AU - Cox,J, AU - Peter,W, AU - Schäfer,R, AU - Johnson,N, AU - McElhinney,L M, AU - Geue,J L, AU - Tjørnehøj,K, AU - Fooks,A R, PY - 2004/3/20/pubmed PY - 2004/5/28/medline PY - 2004/3/20/entrez SP - 49 EP - 54 JF - Journal of veterinary medicine. B, Infectious diseases and veterinary public health JO - J Vet Med B Infect Dis Vet Public Health VL - 51 IS - 2 N2 - European bat lyssavirus type 1 (EBLV-1, genotype 5) is known to endemically circulate in insectivorous bat populations in Germany. In August 2001, a rabies suspect stone marten (Martes foina) was found in the city of Burg (Saxony-Anhalt, Germany) and was sent to the regional veterinary laboratory for routine rabies diagnosis. Whereas brain samples repeatedly tested negative in the fluorescent antibody test for classical rabies virus (genotype 1), the mouse inoculation test and the rabies tissue culture inoculation test yielded positive results. Rabies viral RNA was also detected in the stone marten brain sample both by nested and heminested RT-PCR specific for the nucleoprotein gene and for the nucleoprotein phosphoprotein junction of rabies virus. The amplification products were sequenced to genotype the isolate. Sequence data obtained from the first-round RT-PCR products were analysed and the suspect stone marten isolate was confirmed as a rabies related virus (EBLV-1a). Phylogenetic comparison with sequences from recent genotype five isolates from Germany and Denmark showed that it was closely related to a previous isolate of EBLV-1 from a serotine bat in Saxony-Anhalt obtained in the same year in an area adjacent to the place where the EBLV-1 infected stone marten was found. Both EBLV-1 isolates share a 99.5% identity. This is the first report of an EBLV-1a spill-over from an insectivorous bat into wildlife in Europe. SN - 0931-1793 UR - https://www.unboundmedicine.com/medline/citation/15030600/Spill_over_of_European_bat_lyssavirus_type_1_into_a_stone_marten__Martes_foina__in_Germany_ L2 - https://onlinelibrary.wiley.com/resolve/openurl?genre=article&sid=nlm:pubmed&issn=0931-1793&date=2004&volume=51&issue=2&spage=49 DB - PRIME DP - Unbound Medicine ER -