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Real-time reverse transcription-polymerase chain reaction assay for SARS-associated coronavirus.
Emerg Infect Dis. 2004 Feb; 10(2):311-6.EI

Abstract

A real-time reverse transcription-polymerase chain reaction (RT-PCR) assay was developed to rapidly detect the severe acute respiratory syndrome-associated coronavirus (SARS-CoV). The assay, based on multiple primer and probe sets located in different regions of the SARS-CoV genome, could discriminate SARS-CoV from other human and animal coronaviruses with a potential detection limit of <10 genomic copies per reaction. The real-time RT-PCR assay was more sensitive than a conventional RT-PCR assay or culture isolation and proved suitable to detect SARS-CoV in clinical specimens. Application of this assay will aid in diagnosing SARS-CoV infection.

Authors+Show Affiliations

Centers for Disease Control and Prevention, Atlanta, Georgia, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Evaluation Study
Journal Article

Language

eng

PubMed ID

15030703

Citation

Emery, Shannon L., et al. "Real-time Reverse Transcription-polymerase Chain Reaction Assay for SARS-associated Coronavirus." Emerging Infectious Diseases, vol. 10, no. 2, 2004, pp. 311-6.
Emery SL, Erdman DD, Bowen MD, et al. Real-time reverse transcription-polymerase chain reaction assay for SARS-associated coronavirus. Emerg Infect Dis. 2004;10(2):311-6.
Emery, S. L., Erdman, D. D., Bowen, M. D., Newton, B. R., Winchell, J. M., Meyer, R. F., Tong, S., Cook, B. T., Holloway, B. P., McCaustland, K. A., Rota, P. A., Bankamp, B., Lowe, L. E., Ksiazek, T. G., Bellini, W. J., & Anderson, L. J. (2004). Real-time reverse transcription-polymerase chain reaction assay for SARS-associated coronavirus. Emerging Infectious Diseases, 10(2), 311-6.
Emery SL, et al. Real-time Reverse Transcription-polymerase Chain Reaction Assay for SARS-associated Coronavirus. Emerg Infect Dis. 2004;10(2):311-6. PubMed PMID: 15030703.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Real-time reverse transcription-polymerase chain reaction assay for SARS-associated coronavirus. AU - Emery,Shannon L, AU - Erdman,Dean D, AU - Bowen,Michael D, AU - Newton,Bruce R, AU - Winchell,Jonas M, AU - Meyer,Richard F, AU - Tong,Suxiang, AU - Cook,Byron T, AU - Holloway,Brian P, AU - McCaustland,Karen A, AU - Rota,Paul A, AU - Bankamp,Bettina, AU - Lowe,Luis E, AU - Ksiazek,Tom G, AU - Bellini,William J, AU - Anderson,Larry J, PY - 2004/3/20/pubmed PY - 2004/5/12/medline PY - 2004/3/20/entrez SP - 311 EP - 6 JF - Emerging infectious diseases JO - Emerg Infect Dis VL - 10 IS - 2 N2 - A real-time reverse transcription-polymerase chain reaction (RT-PCR) assay was developed to rapidly detect the severe acute respiratory syndrome-associated coronavirus (SARS-CoV). The assay, based on multiple primer and probe sets located in different regions of the SARS-CoV genome, could discriminate SARS-CoV from other human and animal coronaviruses with a potential detection limit of <10 genomic copies per reaction. The real-time RT-PCR assay was more sensitive than a conventional RT-PCR assay or culture isolation and proved suitable to detect SARS-CoV in clinical specimens. Application of this assay will aid in diagnosing SARS-CoV infection. SN - 1080-6040 UR - https://www.unboundmedicine.com/medline/citation/15030703/Real_time_reverse_transcription_polymerase_chain_reaction_assay_for_SARS_associated_coronavirus_ L2 - https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/15030703/ DB - PRIME DP - Unbound Medicine ER -