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Ultrastructural characterization of SARS coronavirus.
Emerg Infect Dis. 2004 Feb; 10(2):320-6.EI

Abstract

Severe acute respiratory syndrome (SARS) was first described during a 2002-2003 global outbreak of severe pneumonia associated with human deaths and person-to-person disease transmission. The etiologic agent was initially identified as a coronavirus by thin-section electron microscopic examination of a virus isolate. Virions were spherical, 78 nm in mean diameter, and composed of a helical nucleocapsid within an envelope with surface projections. We show that infection with the SARS-associated coronavirus resulted in distinct ultrastructural features: double-membrane vesicles, nucleocapsid inclusions, and large granular areas of cytoplasm. These three structures and the coronavirus particles were shown to be positive for viral proteins and RNA by using ultrastructural immunogold and in situ hybridization assays. In addition, ultrastructural examination of a bronchiolar lavage specimen from a SARS patient showed numerous coronavirus-infected cells with features similar to those in infected culture cells. Electron microscopic studies were critical in identifying the etiologic agent of the SARS outbreak and in guiding subsequent laboratory and epidemiologic investigations.

Authors+Show Affiliations

Infectious Disease Pathology Activity, Division of Viral and Rickettsial Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia 30333, USA. cgoldsmith@cdc.govNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

15030705

Citation

Goldsmith, Cynthia S., et al. "Ultrastructural Characterization of SARS Coronavirus." Emerging Infectious Diseases, vol. 10, no. 2, 2004, pp. 320-6.
Goldsmith CS, Tatti KM, Ksiazek TG, et al. Ultrastructural characterization of SARS coronavirus. Emerging Infect Dis. 2004;10(2):320-6.
Goldsmith, C. S., Tatti, K. M., Ksiazek, T. G., Rollin, P. E., Comer, J. A., Lee, W. W., Rota, P. A., Bankamp, B., Bellini, W. J., & Zaki, S. R. (2004). Ultrastructural characterization of SARS coronavirus. Emerging Infectious Diseases, 10(2), 320-6.
Goldsmith CS, et al. Ultrastructural Characterization of SARS Coronavirus. Emerging Infect Dis. 2004;10(2):320-6. PubMed PMID: 15030705.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Ultrastructural characterization of SARS coronavirus. AU - Goldsmith,Cynthia S, AU - Tatti,Kathleen M, AU - Ksiazek,Thomas G, AU - Rollin,Pierre E, AU - Comer,James A, AU - Lee,William W, AU - Rota,Paul A, AU - Bankamp,Bettina, AU - Bellini,William J, AU - Zaki,Sherif R, PY - 2004/3/20/pubmed PY - 2004/5/12/medline PY - 2004/3/20/entrez SP - 320 EP - 6 JF - Emerging infectious diseases JO - Emerging Infect. Dis. VL - 10 IS - 2 N2 - Severe acute respiratory syndrome (SARS) was first described during a 2002-2003 global outbreak of severe pneumonia associated with human deaths and person-to-person disease transmission. The etiologic agent was initially identified as a coronavirus by thin-section electron microscopic examination of a virus isolate. Virions were spherical, 78 nm in mean diameter, and composed of a helical nucleocapsid within an envelope with surface projections. We show that infection with the SARS-associated coronavirus resulted in distinct ultrastructural features: double-membrane vesicles, nucleocapsid inclusions, and large granular areas of cytoplasm. These three structures and the coronavirus particles were shown to be positive for viral proteins and RNA by using ultrastructural immunogold and in situ hybridization assays. In addition, ultrastructural examination of a bronchiolar lavage specimen from a SARS patient showed numerous coronavirus-infected cells with features similar to those in infected culture cells. Electron microscopic studies were critical in identifying the etiologic agent of the SARS outbreak and in guiding subsequent laboratory and epidemiologic investigations. SN - 1080-6040 UR - https://www.unboundmedicine.com/medline/citation/15030705/full_citation L2 - https://www.cdc.gov/ncidod/EID/vol10no2/03-0913.htm DB - PRIME DP - Unbound Medicine ER -