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Relative rates of non-pneumonic SARS coronavirus infection and SARS coronavirus pneumonia.
Lancet. 2004 Mar 13; 363(9412):841-5.Lct

Abstract

BACKGROUND

Although the genome of severe acute respiratory syndrome coronavirus (SARS-CoV) has been sequenced and a possible animal reservoir identified, seroprevalence studies and mass screening for detection of subclinical and non-pneumonic infections are still lacking.

METHODS

We cloned and purified the nucleocapsid protein and spike polypeptide of SARS-CoV and examined their immunogenicity with serum from patients with SARS-CoV pneumonia. An ELISA based on recombinant nucleocapsid protein for IgG detection was tested with serum from 149 healthy blood donors who donated 3 years previously and with serum positive for antibodies against SARS-CoV (by indirect immunofluorescence assay) from 106 patients with SARS-CoV pneumonia. The seroprevalence of SARS-CoV was studied with the ELISA in healthy blood donors who donated during the SARS outbreak in Hong Kong, non-pneumonic hospital inpatients, and symptom-free health-care workers. All positive samples were confirmed by two separate western-blot assays (with recombinant nucleocapsid protein and recombinant spike polypeptide).

FINDINGS

Western-blot analysis showed that the nucleocapsid protein and spike polypeptide of SARS-CoV are highly immunogenic. The specificity of the IgG antibody test (ELISA with positive samples confirmed by the two western-blot assays) was 100%, and the sensitivity was 94.3%. Three of 400 healthy blood donors who donated during the SARS outbreak and one of 131 non-pneumonic paediatric inpatients were positive for IgG antibodies, confirmed by the two western-blot assays (total, 0.48% of our study population).

INTERPRETATION

Our findings support the existence of subclinical or non-pneumonic SARS-CoV infections. Such infections are more common than SARS-CoV pneumonia in our locality.

Authors+Show Affiliations

Department of Microbiology, University of Hong Kong, University Pathology Building, Queen Mary Hospital, Hong Kong.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15031027

Citation

Woo, Patrick C Y., et al. "Relative Rates of Non-pneumonic SARS Coronavirus Infection and SARS Coronavirus Pneumonia." Lancet (London, England), vol. 363, no. 9412, 2004, pp. 841-5.
Woo PC, Lau SK, Tsoi HW, et al. Relative rates of non-pneumonic SARS coronavirus infection and SARS coronavirus pneumonia. Lancet. 2004;363(9412):841-5.
Woo, P. C., Lau, S. K., Tsoi, H. W., Chan, K. H., Wong, B. H., Che, X. Y., Tam, V. K., Tam, S. C., Cheng, V. C., Hung, I. F., Wong, S. S., Zheng, B. J., Guan, Y., & Yuen, K. Y. (2004). Relative rates of non-pneumonic SARS coronavirus infection and SARS coronavirus pneumonia. Lancet (London, England), 363(9412), 841-5.
Woo PC, et al. Relative Rates of Non-pneumonic SARS Coronavirus Infection and SARS Coronavirus Pneumonia. Lancet. 2004 Mar 13;363(9412):841-5. PubMed PMID: 15031027.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Relative rates of non-pneumonic SARS coronavirus infection and SARS coronavirus pneumonia. AU - Woo,Patrick C Y, AU - Lau,Susanna K P, AU - Tsoi,Hoi-wah, AU - Chan,Kwok-hung, AU - Wong,Beatrice H L, AU - Che,Xiao-yan, AU - Tam,Victoria K P, AU - Tam,Sidney C F, AU - Cheng,Vincent C C, AU - Hung,Ivan F N, AU - Wong,Samson S Y, AU - Zheng,Bo-jian, AU - Guan,Yi, AU - Yuen,Kwok-yung, PY - 2004/3/20/pubmed PY - 2004/4/20/medline PY - 2004/3/20/entrez SP - 841 EP - 5 JF - Lancet (London, England) JO - Lancet VL - 363 IS - 9412 N2 - BACKGROUND: Although the genome of severe acute respiratory syndrome coronavirus (SARS-CoV) has been sequenced and a possible animal reservoir identified, seroprevalence studies and mass screening for detection of subclinical and non-pneumonic infections are still lacking. METHODS: We cloned and purified the nucleocapsid protein and spike polypeptide of SARS-CoV and examined their immunogenicity with serum from patients with SARS-CoV pneumonia. An ELISA based on recombinant nucleocapsid protein for IgG detection was tested with serum from 149 healthy blood donors who donated 3 years previously and with serum positive for antibodies against SARS-CoV (by indirect immunofluorescence assay) from 106 patients with SARS-CoV pneumonia. The seroprevalence of SARS-CoV was studied with the ELISA in healthy blood donors who donated during the SARS outbreak in Hong Kong, non-pneumonic hospital inpatients, and symptom-free health-care workers. All positive samples were confirmed by two separate western-blot assays (with recombinant nucleocapsid protein and recombinant spike polypeptide). FINDINGS: Western-blot analysis showed that the nucleocapsid protein and spike polypeptide of SARS-CoV are highly immunogenic. The specificity of the IgG antibody test (ELISA with positive samples confirmed by the two western-blot assays) was 100%, and the sensitivity was 94.3%. Three of 400 healthy blood donors who donated during the SARS outbreak and one of 131 non-pneumonic paediatric inpatients were positive for IgG antibodies, confirmed by the two western-blot assays (total, 0.48% of our study population). INTERPRETATION: Our findings support the existence of subclinical or non-pneumonic SARS-CoV infections. Such infections are more common than SARS-CoV pneumonia in our locality. SN - 1474-547X UR - https://www.unboundmedicine.com/medline/citation/15031027/Relative_rates_of_non_pneumonic_SARS_coronavirus_infection_and_SARS_coronavirus_pneumonia_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0140-6736(04)15729-2 DB - PRIME DP - Unbound Medicine ER -