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The effect of dietary docosahexaenoic acid on the expression of porcine lipid metabolism-related genes.
J Anim Sci. 2004 Mar; 82(3):683-9.JA

Abstract

To study the effect of dietary docosahexaenoic acid (DHA) on the expression of adipocyte determination and differentiation-dependent factor 1 (ADD1) mRNA in pig tissues, weaned, crossbred pigs (30 d of age) were fed either 2% (as-fed basis) tallow or DHA oil for 18 d. Body weight of the pigs was not affected by different dietary fatty acid (FA) compositions. The plasma and liver FA composition reflected the composition of the diet. The adipose tissue and skeletal muscle FA composition only partially reflected the diet, indicating either a slower FA turnover or that a greater proportion of the FA in these tissues is from endogenous FA synthesis. The ADD1 is an important transcription factor that modulates transcription of FA synthase to regulate the endogenous FA synthesis in the liver and adipose tissue. The ADD1 mRNA was decreased (P < 0.05) in the liver of DHA-treated pigs compared with that of the tallow-treated pigs. The diets did not have an effect on the ADD1 mRNA in pig adipose tissue. The ADD1 transcript was not detected in pig skeletal muscle. These results indicate that significant enrichment of liver DHA content inhibits the expression of ADD1 mRNA. Such an effect is similar to that reported in porcine differentiating adipocytes cultured with DHA. The liver and muscle acyl CoA oxidase mRNA concentration was increased (P < 0.05) by DHA oil treatment, suggesting that DHA treatment may increase peroxisomal fatty acid oxidation in these two tissues. Our present observations demonstrate that dietary DHA enrichment not only affects tissue DHA concentration but also mildly modifies the expression of genes related to fatty acid metabolism in the porcine liver and skeletal muscle.

Authors+Show Affiliations

Department of Animal Science, National Taiwan University, Taipei 106, Taiwan.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15032424

Citation

Hsu, J M., et al. "The Effect of Dietary Docosahexaenoic Acid On the Expression of Porcine Lipid Metabolism-related Genes." Journal of Animal Science, vol. 82, no. 3, 2004, pp. 683-9.
Hsu JM, Wang PH, Liu BH, et al. The effect of dietary docosahexaenoic acid on the expression of porcine lipid metabolism-related genes. J Anim Sci. 2004;82(3):683-9.
Hsu, J. M., Wang, P. H., Liu, B. H., & Ding, S. T. (2004). The effect of dietary docosahexaenoic acid on the expression of porcine lipid metabolism-related genes. Journal of Animal Science, 82(3), 683-9.
Hsu JM, et al. The Effect of Dietary Docosahexaenoic Acid On the Expression of Porcine Lipid Metabolism-related Genes. J Anim Sci. 2004;82(3):683-9. PubMed PMID: 15032424.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The effect of dietary docosahexaenoic acid on the expression of porcine lipid metabolism-related genes. AU - Hsu,J M, AU - Wang,P H, AU - Liu,B H, AU - Ding,S T, PY - 2004/3/23/pubmed PY - 2004/8/20/medline PY - 2004/3/23/entrez SP - 683 EP - 9 JF - Journal of animal science JO - J Anim Sci VL - 82 IS - 3 N2 - To study the effect of dietary docosahexaenoic acid (DHA) on the expression of adipocyte determination and differentiation-dependent factor 1 (ADD1) mRNA in pig tissues, weaned, crossbred pigs (30 d of age) were fed either 2% (as-fed basis) tallow or DHA oil for 18 d. Body weight of the pigs was not affected by different dietary fatty acid (FA) compositions. The plasma and liver FA composition reflected the composition of the diet. The adipose tissue and skeletal muscle FA composition only partially reflected the diet, indicating either a slower FA turnover or that a greater proportion of the FA in these tissues is from endogenous FA synthesis. The ADD1 is an important transcription factor that modulates transcription of FA synthase to regulate the endogenous FA synthesis in the liver and adipose tissue. The ADD1 mRNA was decreased (P < 0.05) in the liver of DHA-treated pigs compared with that of the tallow-treated pigs. The diets did not have an effect on the ADD1 mRNA in pig adipose tissue. The ADD1 transcript was not detected in pig skeletal muscle. These results indicate that significant enrichment of liver DHA content inhibits the expression of ADD1 mRNA. Such an effect is similar to that reported in porcine differentiating adipocytes cultured with DHA. The liver and muscle acyl CoA oxidase mRNA concentration was increased (P < 0.05) by DHA oil treatment, suggesting that DHA treatment may increase peroxisomal fatty acid oxidation in these two tissues. Our present observations demonstrate that dietary DHA enrichment not only affects tissue DHA concentration but also mildly modifies the expression of genes related to fatty acid metabolism in the porcine liver and skeletal muscle. SN - 0021-8812 UR - https://www.unboundmedicine.com/medline/citation/15032424/The_effect_of_dietary_docosahexaenoic_acid_on_the_expression_of_porcine_lipid_metabolism_related_genes_ L2 - https://academic.oup.com/jas/article-lookup/doi/10.2527/2004.823683x DB - PRIME DP - Unbound Medicine ER -