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Expression of estrogen receptors alpha and beta in the fetal baboon testis and epididymis.
Biol Reprod. 2004 Apr; 70(4):1106-13.BR

Abstract

Although studies in transgenic mice suggest that estrogen is important for development of the testis, very little is known about the potential role of estrogen in maturation of the primate fetal testis. Therefore, as a first step to determine whether estrogen regulates maturation of the fetal primate testis, we used immunocytochemistry to determine estrogen receptor (ER) alpha and beta expression in the fetal baboon testis. Second, we established methods to quantify ERbeta mRNA levels by competitive reverse transcription-polymerase chain reaction in Sertoli cells isolated by laser capture microdissection (LCM) from the fetal baboon testis. ERbeta protein expression was abundant in the nuclei of Sertoli, peritubular, and interstitial cells in baboon fetuses at mid (Day 100) and late (Day 165) gestation (term is 184 days). ERbeta mRNA level was 0.03 attomole/femtomole 18S rRNA in Sertoli cell nuclei and associated cytoplasm isolated by LCM. ERalpha was expressed in low level in seminiferous tubules and in moderate level in peritubular cells on Day 165. Germ cells expressed very little ERalpha or ERbeta protein, whereas the baboon fetal epididymis exhibited extensive ERalpha and ERbeta immunostaining at mid- and late gestation. In contrast to the robust expression of ERbeta, androgen receptor protein was not demonstrable within the cells of the seminiferous cords but was abundantly expressed in epididymal epithelial cells of the fetal baboon. In summary, the results of this study show that the fetal baboon testis and epididymis expressed the ERalpha and ERbeta, and we suggest that our nonhuman primate baboon model can be used to study the potential role of estrogen on maturation of the fetal testis.

Authors+Show Affiliations

Departments of Obstetrics, Gynecology, Reproductive Sciences, and Physiology, Center for Studies in Reproduction, University of Maryland School of Medicine, Baltimore, Maryland 21201, USA. ealbrech@umaryland.eduNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

15033877

Citation

Albrecht, Eugene D., et al. "Expression of Estrogen Receptors Alpha and Beta in the Fetal Baboon Testis and Epididymis." Biology of Reproduction, vol. 70, no. 4, 2004, pp. 1106-13.
Albrecht ED, Billiar RB, Aberdeen GW, et al. Expression of estrogen receptors alpha and beta in the fetal baboon testis and epididymis. Biol Reprod. 2004;70(4):1106-13.
Albrecht, E. D., Billiar, R. B., Aberdeen, G. W., Babischkin, J. S., & Pepe, G. J. (2004). Expression of estrogen receptors alpha and beta in the fetal baboon testis and epididymis. Biology of Reproduction, 70(4), 1106-13.
Albrecht ED, et al. Expression of Estrogen Receptors Alpha and Beta in the Fetal Baboon Testis and Epididymis. Biol Reprod. 2004;70(4):1106-13. PubMed PMID: 15033877.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Expression of estrogen receptors alpha and beta in the fetal baboon testis and epididymis. AU - Albrecht,Eugene D, AU - Billiar,Reinhart B, AU - Aberdeen,Graham W, AU - Babischkin,Jeffery S, AU - Pepe,Gerald J, Y1 - 2003/12/10/ PY - 2004/3/23/pubmed PY - 2004/11/4/medline PY - 2004/3/23/entrez SP - 1106 EP - 13 JF - Biology of reproduction JO - Biol Reprod VL - 70 IS - 4 N2 - Although studies in transgenic mice suggest that estrogen is important for development of the testis, very little is known about the potential role of estrogen in maturation of the primate fetal testis. Therefore, as a first step to determine whether estrogen regulates maturation of the fetal primate testis, we used immunocytochemistry to determine estrogen receptor (ER) alpha and beta expression in the fetal baboon testis. Second, we established methods to quantify ERbeta mRNA levels by competitive reverse transcription-polymerase chain reaction in Sertoli cells isolated by laser capture microdissection (LCM) from the fetal baboon testis. ERbeta protein expression was abundant in the nuclei of Sertoli, peritubular, and interstitial cells in baboon fetuses at mid (Day 100) and late (Day 165) gestation (term is 184 days). ERbeta mRNA level was 0.03 attomole/femtomole 18S rRNA in Sertoli cell nuclei and associated cytoplasm isolated by LCM. ERalpha was expressed in low level in seminiferous tubules and in moderate level in peritubular cells on Day 165. Germ cells expressed very little ERalpha or ERbeta protein, whereas the baboon fetal epididymis exhibited extensive ERalpha and ERbeta immunostaining at mid- and late gestation. In contrast to the robust expression of ERbeta, androgen receptor protein was not demonstrable within the cells of the seminiferous cords but was abundantly expressed in epididymal epithelial cells of the fetal baboon. In summary, the results of this study show that the fetal baboon testis and epididymis expressed the ERalpha and ERbeta, and we suggest that our nonhuman primate baboon model can be used to study the potential role of estrogen on maturation of the fetal testis. SN - 0006-3363 UR - https://www.unboundmedicine.com/medline/citation/15033877/Expression_of_estrogen_receptors_alpha_and_beta_in_the_fetal_baboon_testis_and_epididymis_ L2 - https://academic.oup.com/biolreprod/article-lookup/doi/10.1095/biolreprod.103.022665 DB - PRIME DP - Unbound Medicine ER -