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Crystallization and X-ray analysis of bovine glycolipid transfer protein.
Acta Crystallogr D Biol Crystallogr. 2004 Apr; 60(Pt 4):703-5.AC

Abstract

Glycolipid-transfer protein (GLTP) is a 24 kDa basic cytosolic protein that facilitates the transfer of glycolipids between bilayer membranes in vitro, but its in vivo function is unknown. Human, bovine, porcine and murine GLTPs have recently been cloned and share high sequence identity to each other. The three-dimensional structure of GLTP has not yet been solved and no structures of any proteins related to GLTP are known. Therefore, the structure of GLTP might reveal a currently unknown fold. Here, the crystallization and preliminary X-ray analysis of bovine GLTP are reported for the first time. Protein prepared by recombinant techniques using an Escherichia coli expression system has been crystallized using the vapour-diffusion method. The crystals belong to space group P2(1), with unit-cell parameters a = 55.4, b = 34.9, c = 58.5 A, alpha = gamma = 90, beta = 116 degrees. The crystals diffract to 1.6 A resolution and a 97.1% complete data set with an R(merge) of 6.7% has been collected from a single crystal at 100 K using synchrotron radiation.

Authors+Show Affiliations

West G
Department of Biochemistry and Pharmacy, Abo Akademi University, PO Box 66, FIN-20521, Turku, Finland, Finland.
Nymalm Y
No affiliation info available
Airenne TT
No affiliation info available
Kidron H
No affiliation info available
Mattjus P
No affiliation info available
Salminen TT
No affiliation info available

MeSH

AnimalsCarrier ProteinsCattleCloning, MolecularCrystallizationCrystallography, X-RayProtein Conformation

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15039559

Citation

West, Gun, et al. "Crystallization and X-ray Analysis of Bovine Glycolipid Transfer Protein." Acta Crystallographica. Section D, Biological Crystallography, vol. 60, no. Pt 4, 2004, pp. 703-5.
West G, Nymalm Y, Airenne TT, et al. Crystallization and X-ray analysis of bovine glycolipid transfer protein. Acta Crystallogr D Biol Crystallogr. 2004;60(Pt 4):703-5.
West, G., Nymalm, Y., Airenne, T. T., Kidron, H., Mattjus, P., & Salminen, T. T. (2004). Crystallization and X-ray analysis of bovine glycolipid transfer protein. Acta Crystallographica. Section D, Biological Crystallography, 60(Pt 4), 703-5.
West G, et al. Crystallization and X-ray Analysis of Bovine Glycolipid Transfer Protein. Acta Crystallogr D Biol Crystallogr. 2004;60(Pt 4):703-5. PubMed PMID: 15039559.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Crystallization and X-ray analysis of bovine glycolipid transfer protein. AU - West,Gun, AU - Nymalm,Yvonne, AU - Airenne,Tomi T, AU - Kidron,Heidi, AU - Mattjus,Peter, AU - Salminen,Tiina T, Y1 - 2004/03/23/ PY - 2003/12/05/received PY - 2004/01/14/accepted PY - 2004/3/25/pubmed PY - 2004/12/16/medline PY - 2004/3/25/entrez SP - 703 EP - 5 JF - Acta crystallographica. Section D, Biological crystallography JO - Acta Crystallogr D Biol Crystallogr VL - 60 IS - Pt 4 N2 - Glycolipid-transfer protein (GLTP) is a 24 kDa basic cytosolic protein that facilitates the transfer of glycolipids between bilayer membranes in vitro, but its in vivo function is unknown. Human, bovine, porcine and murine GLTPs have recently been cloned and share high sequence identity to each other. The three-dimensional structure of GLTP has not yet been solved and no structures of any proteins related to GLTP are known. Therefore, the structure of GLTP might reveal a currently unknown fold. Here, the crystallization and preliminary X-ray analysis of bovine GLTP are reported for the first time. Protein prepared by recombinant techniques using an Escherichia coli expression system has been crystallized using the vapour-diffusion method. The crystals belong to space group P2(1), with unit-cell parameters a = 55.4, b = 34.9, c = 58.5 A, alpha = gamma = 90, beta = 116 degrees. The crystals diffract to 1.6 A resolution and a 97.1% complete data set with an R(merge) of 6.7% has been collected from a single crystal at 100 K using synchrotron radiation. SN - 0907-4449 UR - https://www.unboundmedicine.com/medline/citation/15039559/Crystallization_and_X_ray_analysis_of_bovine_glycolipid_transfer_protein_ DB - PRIME DP - Unbound Medicine ER -