Tags

Type your tag names separated by a space and hit enter

Cloning and sequencing of putative calreticulin complementary DNAs from four hard tick species.
J Parasitol. 2004 Feb; 90(1):73-8.JP

Abstract

Calreticulin (CRT) is a calcium-binding protein and has many functions in eukaryotic cells. CRT is possibly involved in parasite host immune system evasion. To better understand the molecular basis of CRT in ticks, we cloned and sequenced 4 full-length complementary DNAs (cDNAs) from the hard tick species, Dermacentor variabilis, Haemaphysalis longicornis, Ixodes scapularis, and Rhipicephalus sanguineus, using the technique of rapid amplification of cDNA ends. The deduced amino acid sequences share high identities (between 77 and 98%) with 3 known tick CRT sequences. The major characteristics of known CRTs are observed in all 4 of our deduced tick CRTs. These include 3 major domains, a signal peptide sequence at the beginning of the coding region, 2 triplets of conserved regions, cysteine sites providing disulfide bridges for N-terminal folding, and a nuclear localization signal. Remarkably, the replacement of the endoplasmic reticulum retention signal KDEL by HEEL, which is believed to be associated with secretion of CRT into the host during feeding and was previously recorded only in 2 ticks and a hookworm, is also present in all 4 of our tick putative CRTs. In addition, the CRT gene is potentially useful for tick phylogenetic reconstruction.

Authors+Show Affiliations

Department of Biology and Institute of Arthropodology and Parasitology, Georgia Southern University, Statesboro, Georgia 30460-8042, USA.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

15040669

Citation

Xu, Guang, et al. "Cloning and Sequencing of Putative Calreticulin Complementary DNAs From Four Hard Tick Species." The Journal of Parasitology, vol. 90, no. 1, 2004, pp. 73-8.
Xu G, Fang QQ, Keirans JE, et al. Cloning and sequencing of putative calreticulin complementary DNAs from four hard tick species. J Parasitol. 2004;90(1):73-8.
Xu, G., Fang, Q. Q., Keirans, J. E., & Durden, L. A. (2004). Cloning and sequencing of putative calreticulin complementary DNAs from four hard tick species. The Journal of Parasitology, 90(1), 73-8.
Xu G, et al. Cloning and Sequencing of Putative Calreticulin Complementary DNAs From Four Hard Tick Species. J Parasitol. 2004;90(1):73-8. PubMed PMID: 15040669.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Cloning and sequencing of putative calreticulin complementary DNAs from four hard tick species. AU - Xu,Guang, AU - Fang,Quentin Q, AU - Keirans,James E, AU - Durden,Lance A, PY - 2004/3/26/pubmed PY - 2004/4/7/medline PY - 2004/3/26/entrez SP - 73 EP - 8 JF - The Journal of parasitology JO - J. Parasitol. VL - 90 IS - 1 N2 - Calreticulin (CRT) is a calcium-binding protein and has many functions in eukaryotic cells. CRT is possibly involved in parasite host immune system evasion. To better understand the molecular basis of CRT in ticks, we cloned and sequenced 4 full-length complementary DNAs (cDNAs) from the hard tick species, Dermacentor variabilis, Haemaphysalis longicornis, Ixodes scapularis, and Rhipicephalus sanguineus, using the technique of rapid amplification of cDNA ends. The deduced amino acid sequences share high identities (between 77 and 98%) with 3 known tick CRT sequences. The major characteristics of known CRTs are observed in all 4 of our deduced tick CRTs. These include 3 major domains, a signal peptide sequence at the beginning of the coding region, 2 triplets of conserved regions, cysteine sites providing disulfide bridges for N-terminal folding, and a nuclear localization signal. Remarkably, the replacement of the endoplasmic reticulum retention signal KDEL by HEEL, which is believed to be associated with secretion of CRT into the host during feeding and was previously recorded only in 2 ticks and a hookworm, is also present in all 4 of our tick putative CRTs. In addition, the CRT gene is potentially useful for tick phylogenetic reconstruction. SN - 0022-3395 UR - https://www.unboundmedicine.com/medline/citation/15040669/Cloning_and_sequencing_of_putative_calreticulin_complementary_DNAs_from_four_hard_tick_species_ L2 - http://www.journalofparasitology.org/doi/10.1645/GE-157R?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -