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Inorganic polyphosphate in mitochondria of Saccharomyces cerevisiae at phosphate limitation and phosphate excess.
FEMS Yeast Res. 2004 Mar; 4(6):643-8.FY

Abstract

Isolated mitochondria of Saccharomyces cerevisiae cells grown on glucose possess acid-soluble inorganic polyphosphate (polyP). Its level strongly depends on phosphate (P(i)) concentration in the culture medium. The polyP level in mitochondria showed 11-fold decrease under 0.8 mM P(i) as compared with 19.3 mM P(i). When spheroplasts isolated from P(i)-starved cells were incubated in the P(i)-complete medium, they accumulated polyP and exhibited a phosphate overplus effect. Under phosphate overplus the polyP level in mitochondria was two times higher than in the complete medium without preliminary P(i) starvation. The average chain length of polyP in mitochondria was of <15 phosphate residues at 19.3 mM P(i) in the culture medium and increased at phosphate overplus. Deoxyglucose inhibited polyP accumulation in spheroplasts, but had no effect on polyP accumulation in mitochondria. Uncouplers (FCCP, dinitrophenol) and ionophores (monensin, nigericin) inhibited polyP accumulation in mitochondria more efficiently than in spheroplasts. Fast hydrolysis of polyP was observed after sonication of isolated mitochondria. Probably, the accumulation of polyP in mitochondria depended on the proton-motive force of their membranes.

Authors+Show Affiliations

Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, Pushchino 142290, Moscow Region, Russia.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15040953

Citation

Pestov, Nikolay A., et al. "Inorganic Polyphosphate in Mitochondria of Saccharomyces Cerevisiae at Phosphate Limitation and Phosphate Excess." FEMS Yeast Research, vol. 4, no. 6, 2004, pp. 643-8.
Pestov NA, Kulakovskaya TV, Kulaev IS. Inorganic polyphosphate in mitochondria of Saccharomyces cerevisiae at phosphate limitation and phosphate excess. FEMS Yeast Res. 2004;4(6):643-8.
Pestov, N. A., Kulakovskaya, T. V., & Kulaev, I. S. (2004). Inorganic polyphosphate in mitochondria of Saccharomyces cerevisiae at phosphate limitation and phosphate excess. FEMS Yeast Research, 4(6), 643-8.
Pestov NA, Kulakovskaya TV, Kulaev IS. Inorganic Polyphosphate in Mitochondria of Saccharomyces Cerevisiae at Phosphate Limitation and Phosphate Excess. FEMS Yeast Res. 2004;4(6):643-8. PubMed PMID: 15040953.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Inorganic polyphosphate in mitochondria of Saccharomyces cerevisiae at phosphate limitation and phosphate excess. AU - Pestov,Nikolay A, AU - Kulakovskaya,Tatyana V, AU - Kulaev,Igor S, PY - 2003/11/12/received PY - 2003/12/20/revised PY - 2003/12/29/accepted PY - 2004/3/26/pubmed PY - 2004/6/4/medline PY - 2004/3/26/entrez SP - 643 EP - 8 JF - FEMS yeast research JO - FEMS Yeast Res VL - 4 IS - 6 N2 - Isolated mitochondria of Saccharomyces cerevisiae cells grown on glucose possess acid-soluble inorganic polyphosphate (polyP). Its level strongly depends on phosphate (P(i)) concentration in the culture medium. The polyP level in mitochondria showed 11-fold decrease under 0.8 mM P(i) as compared with 19.3 mM P(i). When spheroplasts isolated from P(i)-starved cells were incubated in the P(i)-complete medium, they accumulated polyP and exhibited a phosphate overplus effect. Under phosphate overplus the polyP level in mitochondria was two times higher than in the complete medium without preliminary P(i) starvation. The average chain length of polyP in mitochondria was of <15 phosphate residues at 19.3 mM P(i) in the culture medium and increased at phosphate overplus. Deoxyglucose inhibited polyP accumulation in spheroplasts, but had no effect on polyP accumulation in mitochondria. Uncouplers (FCCP, dinitrophenol) and ionophores (monensin, nigericin) inhibited polyP accumulation in mitochondria more efficiently than in spheroplasts. Fast hydrolysis of polyP was observed after sonication of isolated mitochondria. Probably, the accumulation of polyP in mitochondria depended on the proton-motive force of their membranes. SN - 1567-1356 UR - https://www.unboundmedicine.com/medline/citation/15040953/Inorganic_polyphosphate_in_mitochondria_of_Saccharomyces_cerevisiae_at_phosphate_limitation_and_phosphate_excess_ DB - PRIME DP - Unbound Medicine ER -