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Control of platelet activation by cyclic AMP turnover and cyclic nucleotide phosphodiesterase type-3.
Biochem Pharmacol. 2004 Apr 15; 67(8):1559-67.BP

Abstract

Prostaglandin-induced cAMP elevation restrains key signaling pathways in platelet activation including Ca(2+) mobilization and integrin alphaIIbbeta3 affinity regulation. We investigated how cAMP turnover by cyclic nucleotide phosphodiesterases (PDEs) regulates platelet activation. In washed human platelets, inhibition of all PDEs and also specific inhibition of PDE3 but not of PDE5 suppressed thrombin-induced Ca(2+) responses. The effect of general PDE or PDE3 inhibition was accompanied by an increase in cAMP, and potentiated by Gs stimulation with prostaglandin E(1). In platelet-rich plasma, general or PDE3 inhibition blocked platelet aggregation, integrin activation, secretion and thrombin generation. In contrast, inhibition of PDE5 increased the cGMP level, but without significant influence on aggregation, alphaIIbbeta3 activation, secretion or procoagulant activity. Nitroprusside (nitric oxide) potentiated the effect of PDE5 inhibition in elevating cGMP. Nitroprusside inhibited platelet responses, but this was accompanied by elevation of cAMP. Together, these results indicate that cAMP is persistently formed in platelets, independently of agonist-induced Gs stimulation. PDE3 thus functions to keep cAMP at a low equilibrium level and reduce the cAMP-regulated threshold for platelet activation. This crucial role of PDE3, but not of PDE5, extends to all major processes in thrombus formation: assembly of platelets into aggregates, secretion of autocrine products, and procoagulant activity.

Authors+Show Affiliations

Department of Biochemistry, University of Maastricht, P.O. Box 616, Maastricht, MD 6200, The Netherlands. jwm.heemskerk@bioch.unimaas.nlNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15041473

Citation

Feijge, Marion A H., et al. "Control of Platelet Activation By Cyclic AMP Turnover and Cyclic Nucleotide Phosphodiesterase Type-3." Biochemical Pharmacology, vol. 67, no. 8, 2004, pp. 1559-67.
Feijge MA, Ansink K, Vanschoonbeek K, et al. Control of platelet activation by cyclic AMP turnover and cyclic nucleotide phosphodiesterase type-3. Biochem Pharmacol. 2004;67(8):1559-67.
Feijge, M. A., Ansink, K., Vanschoonbeek, K., & Heemskerk, J. W. (2004). Control of platelet activation by cyclic AMP turnover and cyclic nucleotide phosphodiesterase type-3. Biochemical Pharmacology, 67(8), 1559-67.
Feijge MA, et al. Control of Platelet Activation By Cyclic AMP Turnover and Cyclic Nucleotide Phosphodiesterase Type-3. Biochem Pharmacol. 2004 Apr 15;67(8):1559-67. PubMed PMID: 15041473.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Control of platelet activation by cyclic AMP turnover and cyclic nucleotide phosphodiesterase type-3. AU - Feijge,Marion A H, AU - Ansink,Karin, AU - Vanschoonbeek,Kristof, AU - Heemskerk,Johan W M, PY - 2003/09/08/received PY - 2003/12/18/accepted PY - 2004/3/26/pubmed PY - 2004/4/22/medline PY - 2004/3/26/entrez SP - 1559 EP - 67 JF - Biochemical pharmacology JO - Biochem Pharmacol VL - 67 IS - 8 N2 - Prostaglandin-induced cAMP elevation restrains key signaling pathways in platelet activation including Ca(2+) mobilization and integrin alphaIIbbeta3 affinity regulation. We investigated how cAMP turnover by cyclic nucleotide phosphodiesterases (PDEs) regulates platelet activation. In washed human platelets, inhibition of all PDEs and also specific inhibition of PDE3 but not of PDE5 suppressed thrombin-induced Ca(2+) responses. The effect of general PDE or PDE3 inhibition was accompanied by an increase in cAMP, and potentiated by Gs stimulation with prostaglandin E(1). In platelet-rich plasma, general or PDE3 inhibition blocked platelet aggregation, integrin activation, secretion and thrombin generation. In contrast, inhibition of PDE5 increased the cGMP level, but without significant influence on aggregation, alphaIIbbeta3 activation, secretion or procoagulant activity. Nitroprusside (nitric oxide) potentiated the effect of PDE5 inhibition in elevating cGMP. Nitroprusside inhibited platelet responses, but this was accompanied by elevation of cAMP. Together, these results indicate that cAMP is persistently formed in platelets, independently of agonist-induced Gs stimulation. PDE3 thus functions to keep cAMP at a low equilibrium level and reduce the cAMP-regulated threshold for platelet activation. This crucial role of PDE3, but not of PDE5, extends to all major processes in thrombus formation: assembly of platelets into aggregates, secretion of autocrine products, and procoagulant activity. SN - 0006-2952 UR - https://www.unboundmedicine.com/medline/citation/15041473/Control_of_platelet_activation_by_cyclic_AMP_turnover_and_cyclic_nucleotide_phosphodiesterase_type_3_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0006295204000280 DB - PRIME DP - Unbound Medicine ER -