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[Theophylline induces contractures in porcine skeletal muscle preparations with the disposition to malignant hyperthermia].

Abstract

OBJECTIVE

Theophylline, a methylxanthine, leads to an increase of the cytoplasmic Ca(2+)-concentration in the muscle cell. Since the in-vitro contracture test (IVCT) with halothane and caffeine does not distinguish a 100% between malignant hyperthermia susceptible (MHS) and non-susceptible (MHN), we examined the in-vitro effects of theophylline in porcine skeletal muscle preparations.

METHODS

After approval by the local animal care committee ten MHS- and nine MHN-swine were anaesthetized and muscle biopsies taken. For IVCT, muscle specimens were exposed to bolus administrations of theophylline in concentrations of 3.0 respectively 5.0 mmol/l. Muscle contracture development and twitch amplitudes were recorded over a period of 30 minutes. Data are expressed as medians and ranges.

RESULTS

After both theophylline bolus administrations MHS-muscles developed significantly higher contractures compared to the MHN-specimens. The MHS-muscles reached a maximum contracture of 17.0 mN (7.2-59.6 mN) after administration of 3.0 mmol/l theophylline. In comparison, two MHN-specimens showed weak contractures with a maximum of 1.4 mN. The 5.0 mmol/l theophylline IVCT resulted in maximum contractures of 19.1 mN (2.1-39.2 mN) for the MHS-preparations. Just in three MHN-muscles weak contractures of 0.0 mN (0.0-0.8 mN) were recorded. Thus, a significant difference without overlap was revealed for the maximum contracture.

CONCLUSION

Theophylline in concentrations of 3.0 and 5.0 mmol/l revealed a clear difference between MHS- and MHN-porcine muscle preparations. Further examinations on human skeletal muscles are needed to demonstrate the value of theophylline in the IVCT MH-diagnosis.

Authors+Show Affiliations

Klinik und Poliklinik für Anästhesiologie Universitätsklinikum Hamburg-Eppendorf. gerbershagen@uke.uni-hamburg.deNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

English Abstract
Journal Article

Language

ger

PubMed ID

15042504

Citation

Gerbershagen, M U., et al. "[Theophylline Induces Contractures in Porcine Skeletal Muscle Preparations With the Disposition to Malignant Hyperthermia]." Anasthesiologie, Intensivmedizin, Notfallmedizin, Schmerztherapie : AINS, vol. 39, no. 3, 2004, pp. 147-52.
Gerbershagen MU, Fiege M, Weisshorn R, et al. [Theophylline induces contractures in porcine skeletal muscle preparations with the disposition to malignant hyperthermia]. Anasthesiol Intensivmed Notfallmed Schmerzther. 2004;39(3):147-52.
Gerbershagen, M. U., Fiege, M., Weisshorn, R., Kolodzie, K., & Wappler, F. (2004). [Theophylline induces contractures in porcine skeletal muscle preparations with the disposition to malignant hyperthermia]. Anasthesiologie, Intensivmedizin, Notfallmedizin, Schmerztherapie : AINS, 39(3), 147-52.
Gerbershagen MU, et al. [Theophylline Induces Contractures in Porcine Skeletal Muscle Preparations With the Disposition to Malignant Hyperthermia]. Anasthesiol Intensivmed Notfallmed Schmerzther. 2004;39(3):147-52. PubMed PMID: 15042504.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Theophylline induces contractures in porcine skeletal muscle preparations with the disposition to malignant hyperthermia]. AU - Gerbershagen,M U, AU - Fiege,M, AU - Weisshorn,R, AU - Kolodzie,K, AU - Wappler,F, PY - 2004/3/26/pubmed PY - 2004/5/8/medline PY - 2004/3/26/entrez SP - 147 EP - 52 JF - Anasthesiologie, Intensivmedizin, Notfallmedizin, Schmerztherapie : AINS JO - Anasthesiol Intensivmed Notfallmed Schmerzther VL - 39 IS - 3 N2 - OBJECTIVE: Theophylline, a methylxanthine, leads to an increase of the cytoplasmic Ca(2+)-concentration in the muscle cell. Since the in-vitro contracture test (IVCT) with halothane and caffeine does not distinguish a 100% between malignant hyperthermia susceptible (MHS) and non-susceptible (MHN), we examined the in-vitro effects of theophylline in porcine skeletal muscle preparations. METHODS: After approval by the local animal care committee ten MHS- and nine MHN-swine were anaesthetized and muscle biopsies taken. For IVCT, muscle specimens were exposed to bolus administrations of theophylline in concentrations of 3.0 respectively 5.0 mmol/l. Muscle contracture development and twitch amplitudes were recorded over a period of 30 minutes. Data are expressed as medians and ranges. RESULTS: After both theophylline bolus administrations MHS-muscles developed significantly higher contractures compared to the MHN-specimens. The MHS-muscles reached a maximum contracture of 17.0 mN (7.2-59.6 mN) after administration of 3.0 mmol/l theophylline. In comparison, two MHN-specimens showed weak contractures with a maximum of 1.4 mN. The 5.0 mmol/l theophylline IVCT resulted in maximum contractures of 19.1 mN (2.1-39.2 mN) for the MHS-preparations. Just in three MHN-muscles weak contractures of 0.0 mN (0.0-0.8 mN) were recorded. Thus, a significant difference without overlap was revealed for the maximum contracture. CONCLUSION: Theophylline in concentrations of 3.0 and 5.0 mmol/l revealed a clear difference between MHS- and MHN-porcine muscle preparations. Further examinations on human skeletal muscles are needed to demonstrate the value of theophylline in the IVCT MH-diagnosis. SN - 0939-2661 UR - https://www.unboundmedicine.com/medline/citation/15042504/[Theophylline_induces_contractures_in_porcine_skeletal_muscle_preparations_with_the_disposition_to_malignant_hyperthermia]_ L2 - http://www.thieme-connect.com/DOI/DOI?10.1055/s-2004-814328 DB - PRIME DP - Unbound Medicine ER -