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Cytomegalovirus infection after orthotopic liver transplantation is restricted by a pre-existing antiviral immune response of the recipient.
J Med Virol. 2004 May; 73(1):45-53.JM

Abstract

Infection of the liver by the human cytomegalovirus (HCMV) frequently occurs after orthotopic liver transplantation (OLT). However, the role of viral replication and the inflammatory reaction in the development of HCMV-associated liver dysfunction is unclear. To address this question in vivo, 84 liver biopsy specimens from 74 patients who received an orthotopic liver transplant were investigated by immunohistochemical detection of viral antigens and cell type specific marker proteins. The extent of viral replication was correlated with the HCMV antibody status of donor and recipient. HCMV immediate early antigens were found in 25 of 84 liver tissue sections investigated, hepatocytes being the predominant target cells. Bile duct epithelial cells, endothelial cells, mesenchymal cells and sinusoidal lining cells were also found susceptible to HCMV infection. The detection of viral capsid antigens, nuclear inclusions in infected cells and foci of infected cells were suggestive of permissive infection in these cells. In 25 HCMV-positive liver biopsy specimens, the median extent of HCMV infection was 0.33 (0.02-5.67) infected cells/mm(2) liver tissue. Primary infection of liver transplant recipients (D+/R-) was associated with a significantly higher extent of organ involvement as compared to reinfection or reactivation (D+/R+). In contrast, the extent of inflammatory infiltrates in areas of infected liver cells was higher in tissues of patients with pre-existing immunoreactivity (R+) compared to patients without pre-existing immunoreactivity (R-). In conclusion, these results favour the assumption that the immune response to HCMV is effective in restricting viral spread in the liver.

Authors+Show Affiliations

Institut für Medizinische Virologie, Universitätsklinikum Tübingen, Tübingen, Germany. ltbissin@med.uni-tuebingen.deNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

15042647

Citation

Bissinger, A L., et al. "Cytomegalovirus Infection After Orthotopic Liver Transplantation Is Restricted By a Pre-existing Antiviral Immune Response of the Recipient." Journal of Medical Virology, vol. 73, no. 1, 2004, pp. 45-53.
Bissinger AL, Oettle H, Jahn G, et al. Cytomegalovirus infection after orthotopic liver transplantation is restricted by a pre-existing antiviral immune response of the recipient. J Med Virol. 2004;73(1):45-53.
Bissinger, A. L., Oettle, H., Jahn, G., Neuhaus, P., & Sinzger, C. (2004). Cytomegalovirus infection after orthotopic liver transplantation is restricted by a pre-existing antiviral immune response of the recipient. Journal of Medical Virology, 73(1), 45-53.
Bissinger AL, et al. Cytomegalovirus Infection After Orthotopic Liver Transplantation Is Restricted By a Pre-existing Antiviral Immune Response of the Recipient. J Med Virol. 2004;73(1):45-53. PubMed PMID: 15042647.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Cytomegalovirus infection after orthotopic liver transplantation is restricted by a pre-existing antiviral immune response of the recipient. AU - Bissinger,A L, AU - Oettle,H, AU - Jahn,G, AU - Neuhaus,P, AU - Sinzger,C, PY - 2004/3/26/pubmed PY - 2004/6/3/medline PY - 2004/3/26/entrez SP - 45 EP - 53 JF - Journal of medical virology JO - J Med Virol VL - 73 IS - 1 N2 - Infection of the liver by the human cytomegalovirus (HCMV) frequently occurs after orthotopic liver transplantation (OLT). However, the role of viral replication and the inflammatory reaction in the development of HCMV-associated liver dysfunction is unclear. To address this question in vivo, 84 liver biopsy specimens from 74 patients who received an orthotopic liver transplant were investigated by immunohistochemical detection of viral antigens and cell type specific marker proteins. The extent of viral replication was correlated with the HCMV antibody status of donor and recipient. HCMV immediate early antigens were found in 25 of 84 liver tissue sections investigated, hepatocytes being the predominant target cells. Bile duct epithelial cells, endothelial cells, mesenchymal cells and sinusoidal lining cells were also found susceptible to HCMV infection. The detection of viral capsid antigens, nuclear inclusions in infected cells and foci of infected cells were suggestive of permissive infection in these cells. In 25 HCMV-positive liver biopsy specimens, the median extent of HCMV infection was 0.33 (0.02-5.67) infected cells/mm(2) liver tissue. Primary infection of liver transplant recipients (D+/R-) was associated with a significantly higher extent of organ involvement as compared to reinfection or reactivation (D+/R+). In contrast, the extent of inflammatory infiltrates in areas of infected liver cells was higher in tissues of patients with pre-existing immunoreactivity (R+) compared to patients without pre-existing immunoreactivity (R-). In conclusion, these results favour the assumption that the immune response to HCMV is effective in restricting viral spread in the liver. SN - 0146-6615 UR - https://www.unboundmedicine.com/medline/citation/15042647/Cytomegalovirus_infection_after_orthotopic_liver_transplantation_is_restricted_by_a_pre_existing_antiviral_immune_response_of_the_recipient_ L2 - https://doi.org/10.1002/jmv.20060 DB - PRIME DP - Unbound Medicine ER -