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Holo-transcobalamin concentration and transcobalamin saturation reflect recent vitamin B12 absorption better than does serum vitamin B12.
Clin Chem. 2004 Jun; 50(6):1043-9.CC

Abstract

BACKGROUND

We evaluated whether measurement of vitamin B(12)-saturated transcobalamin (holo-TC) concentrations or TC saturation (holo-TC:total TC) reflects active vitamin B(12) absorption in healthy individuals and patients after vitamin B(12) intake.

METHODS

We obtained blood samples from 31 healthy individuals (age range, 25-57 years) before (days -1 and 0) and after (days 1, 2, and 6) oral administration of three 9-microg doses of vitamin B(12). The blood samples from seven patients (age range, 22-39 years) suspected to have decreased vitamin B(12) absorption were obtained before and 1 day after the vitamin B(12) intake. The blood samples were analyzed for vitamin B(12), total TC, and holo-TC. The TC saturation was calculated.

RESULTS

Intraindividual variation was <13% for all measured values, as calculated from samples removed on day -1 and 0. In healthy individuals (n = 31) after intake of vitamin B(12), the maximum median (range) increase (as percentages and absolute values) was in TC saturation [52 (-2% to 128)% and 0.04 (0-0.23) as a fraction], closely followed by holo-TC concentrations [39 (0-108)% and 34 (0-149) pmol/L]. All but one healthy individual had an increase of >/=15% in these markers. Serum vitamin B(12) showed a smaller increase [14 (-8 to 51)% and 36 (-27 to 290) pmol/L]. After vitamin B(12) intake, three patients with Crohn disease had the lowest increases in holo-TC concentration (3, 7, and 14 pmol/L) and in TC saturation (0.004, 0.01, and 0.01) among patients and 30 healthy individuals.

CONCLUSION

Holo-TC concentrations and TC saturation reflect normal vitamin B(12) absorption better than does serum vitamin B(12).

Authors+Show Affiliations

Department of Clinical Biochemistry, AKH, Aarhus University Hospital, Norrebrogade 44, DK-8000 Aarhus C, Denmark. vakurbor@hotmail.comNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15044317

Citation

Bor, Mustafa Vakur, et al. "Holo-transcobalamin Concentration and Transcobalamin Saturation Reflect Recent Vitamin B12 Absorption Better Than Does Serum Vitamin B12." Clinical Chemistry, vol. 50, no. 6, 2004, pp. 1043-9.
Bor MV, Nexø E, Hvas AM. Holo-transcobalamin concentration and transcobalamin saturation reflect recent vitamin B12 absorption better than does serum vitamin B12. Clin Chem. 2004;50(6):1043-9.
Bor, M. V., Nexø, E., & Hvas, A. M. (2004). Holo-transcobalamin concentration and transcobalamin saturation reflect recent vitamin B12 absorption better than does serum vitamin B12. Clinical Chemistry, 50(6), 1043-9.
Bor MV, Nexø E, Hvas AM. Holo-transcobalamin Concentration and Transcobalamin Saturation Reflect Recent Vitamin B12 Absorption Better Than Does Serum Vitamin B12. Clin Chem. 2004;50(6):1043-9. PubMed PMID: 15044317.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Holo-transcobalamin concentration and transcobalamin saturation reflect recent vitamin B12 absorption better than does serum vitamin B12. AU - Bor,Mustafa Vakur, AU - Nexø,Ebba, AU - Hvas,Anne-Mette, Y1 - 2004/03/25/ PY - 2004/3/27/pubmed PY - 2004/7/15/medline PY - 2004/3/27/entrez SP - 1043 EP - 9 JF - Clinical chemistry JO - Clin Chem VL - 50 IS - 6 N2 - BACKGROUND: We evaluated whether measurement of vitamin B(12)-saturated transcobalamin (holo-TC) concentrations or TC saturation (holo-TC:total TC) reflects active vitamin B(12) absorption in healthy individuals and patients after vitamin B(12) intake. METHODS: We obtained blood samples from 31 healthy individuals (age range, 25-57 years) before (days -1 and 0) and after (days 1, 2, and 6) oral administration of three 9-microg doses of vitamin B(12). The blood samples from seven patients (age range, 22-39 years) suspected to have decreased vitamin B(12) absorption were obtained before and 1 day after the vitamin B(12) intake. The blood samples were analyzed for vitamin B(12), total TC, and holo-TC. The TC saturation was calculated. RESULTS: Intraindividual variation was <13% for all measured values, as calculated from samples removed on day -1 and 0. In healthy individuals (n = 31) after intake of vitamin B(12), the maximum median (range) increase (as percentages and absolute values) was in TC saturation [52 (-2% to 128)% and 0.04 (0-0.23) as a fraction], closely followed by holo-TC concentrations [39 (0-108)% and 34 (0-149) pmol/L]. All but one healthy individual had an increase of >/=15% in these markers. Serum vitamin B(12) showed a smaller increase [14 (-8 to 51)% and 36 (-27 to 290) pmol/L]. After vitamin B(12) intake, three patients with Crohn disease had the lowest increases in holo-TC concentration (3, 7, and 14 pmol/L) and in TC saturation (0.004, 0.01, and 0.01) among patients and 30 healthy individuals. CONCLUSION: Holo-TC concentrations and TC saturation reflect normal vitamin B(12) absorption better than does serum vitamin B(12). SN - 0009-9147 UR - https://www.unboundmedicine.com/medline/citation/15044317/Holo_transcobalamin_concentration_and_transcobalamin_saturation_reflect_recent_vitamin_B12_absorption_better_than_does_serum_vitamin_B12_ L2 - https://academic.oup.com/clinchem/article-lookup/doi/10.1373/clinchem.2003.027458 DB - PRIME DP - Unbound Medicine ER -