Tags

Type your tag names separated by a space and hit enter

Effect of dietary fat on fat absorption and concomitant plasma and tissue fat composition in a rat model of short bowel syndrome.
Pediatr Surg Int. 2004 Mar; 20(3):185-91.PS

Abstract

The aim of this study was to investigate the effect of dietary fat on the time course of changes in fat absorption and tissue and plasma lipid composition in a rat model of short bowel syndrome (SBS). Male Sprague-Dawley rats underwent either a bowel transection with re-anastomosis (Sham rats) or 75% small bowel resection (SBS rats). Animals were randomly assigned to one of three groups: Sham rats fed normal chow (Sham-NC), SBS rats fed normal chow (SBS-NC), or SBS rats fed a high-fat diet (SBS-HFD). Rats were sacrificed on day 3 or 14. Body weight, food intake, food clearance (dry fecal mass), and fat clearance (total fecal fat) were measured twice a week. Fat and energy intakes were calculated according to the amount of ingested food. Food and fat absorbability were calculated as intake minus clearance and were expressed as percent of intake. Serum cholesterol, triglyceride, and albumin were measured. Total lipid composition of the liver, epididymal adipose tissue, and the small intestine was determined. Statistical analysis was performed by a Student's test, with p values <0.05 considered significant. Both food and fat absorbability diminished after bowel resection in rats fed NC. This was accompanied by a decrease in body weight gain, plasma triglyceride and protein levels, and total lipid content of the liver at day 3 and of a decrease in adipose tissue at day 14 following operation. SBS-HFD rats experienced a significant increase (p<0.05) in food absorbability after 7 days and fat absorbability after 3 days compared with Sham-NC and SBS-NC rats (p<0.05), as well as increases in serum cholesterol, triglycerides, and glucose compared with SBS-NC rats. On day 14, plasma lipid levels in SBS-HFD rats were not different from SBS-NC or control rats; however, albumin levels were higher. A high-fat diet increased total fat content of the liver early after operation. In conclusion, in a rat model of SBS, an early high-fat diet increased the absorptive capacity of the intestinal remnant as seen by increased food and fat absorbability. These findings suggest a benefit of a high-fat diet on intestinal adaptation in general and on lipid absorption in particular.

Authors+Show Affiliations

Sukhotnik I
Carmel Medical Center, Dept. of Surg. B, 7 Michal str, 34362 Haifa, Israel. igor-dr@internet-zahav.net
Mor-Vaknin N
No affiliation info available
Drongowski RA
No affiliation info available
Coran AG
No affiliation info available
Harmon CM
No affiliation info available

MeSH

Analysis of VarianceAnimalsBody CompositionDietary FatsIntestinal AbsorptionLipid MetabolismMaleRandom AllocationRatsRats, Sprague-DawleyShort Bowel Syndrome

Pub Type(s)

Evaluation Study
Journal Article

Language

eng

PubMed ID

15045516

Citation

Sukhotnik, I, et al. "Effect of Dietary Fat On Fat Absorption and Concomitant Plasma and Tissue Fat Composition in a Rat Model of Short Bowel Syndrome." Pediatric Surgery International, vol. 20, no. 3, 2004, pp. 185-91.
Sukhotnik I, Mor-Vaknin N, Drongowski RA, et al. Effect of dietary fat on fat absorption and concomitant plasma and tissue fat composition in a rat model of short bowel syndrome. Pediatr Surg Int. 2004;20(3):185-91.
Sukhotnik, I., Mor-Vaknin, N., Drongowski, R. A., Coran, A. G., & Harmon, C. M. (2004). Effect of dietary fat on fat absorption and concomitant plasma and tissue fat composition in a rat model of short bowel syndrome. Pediatric Surgery International, 20(3), 185-91.
Sukhotnik I, et al. Effect of Dietary Fat On Fat Absorption and Concomitant Plasma and Tissue Fat Composition in a Rat Model of Short Bowel Syndrome. Pediatr Surg Int. 2004;20(3):185-91. PubMed PMID: 15045516.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effect of dietary fat on fat absorption and concomitant plasma and tissue fat composition in a rat model of short bowel syndrome. AU - Sukhotnik,I, AU - Mor-Vaknin,N, AU - Drongowski,R A, AU - Coran,A G, AU - Harmon,C M, Y1 - 2004/03/25/ PY - 2004/3/27/pubmed PY - 2004/8/21/medline PY - 2004/3/27/entrez SP - 185 EP - 91 JF - Pediatric surgery international JO - Pediatr Surg Int VL - 20 IS - 3 N2 - The aim of this study was to investigate the effect of dietary fat on the time course of changes in fat absorption and tissue and plasma lipid composition in a rat model of short bowel syndrome (SBS). Male Sprague-Dawley rats underwent either a bowel transection with re-anastomosis (Sham rats) or 75% small bowel resection (SBS rats). Animals were randomly assigned to one of three groups: Sham rats fed normal chow (Sham-NC), SBS rats fed normal chow (SBS-NC), or SBS rats fed a high-fat diet (SBS-HFD). Rats were sacrificed on day 3 or 14. Body weight, food intake, food clearance (dry fecal mass), and fat clearance (total fecal fat) were measured twice a week. Fat and energy intakes were calculated according to the amount of ingested food. Food and fat absorbability were calculated as intake minus clearance and were expressed as percent of intake. Serum cholesterol, triglyceride, and albumin were measured. Total lipid composition of the liver, epididymal adipose tissue, and the small intestine was determined. Statistical analysis was performed by a Student's test, with p values <0.05 considered significant. Both food and fat absorbability diminished after bowel resection in rats fed NC. This was accompanied by a decrease in body weight gain, plasma triglyceride and protein levels, and total lipid content of the liver at day 3 and of a decrease in adipose tissue at day 14 following operation. SBS-HFD rats experienced a significant increase (p<0.05) in food absorbability after 7 days and fat absorbability after 3 days compared with Sham-NC and SBS-NC rats (p<0.05), as well as increases in serum cholesterol, triglycerides, and glucose compared with SBS-NC rats. On day 14, plasma lipid levels in SBS-HFD rats were not different from SBS-NC or control rats; however, albumin levels were higher. A high-fat diet increased total fat content of the liver early after operation. In conclusion, in a rat model of SBS, an early high-fat diet increased the absorptive capacity of the intestinal remnant as seen by increased food and fat absorbability. These findings suggest a benefit of a high-fat diet on intestinal adaptation in general and on lipid absorption in particular. SN - 0179-0358 UR - https://www.unboundmedicine.com/medline/citation/15045516/Effect_of_dietary_fat_on_fat_absorption_and_concomitant_plasma_and_tissue_fat_composition_in_a_rat_model_of_short_bowel_syndrome_ DB - PRIME DP - Unbound Medicine ER -