Tags

Type your tag names separated by a space and hit enter

The synthesis and distribution of the kinin B1 and B2 receptors are modified in the hippocampus of rats submitted to pilocarpine model of epilepsy.
Brain Res. 2004 Apr 23; 1006(1):114-25.BR

Abstract

Kinins, a special class of polypeptides, are represented by bradykinin (BK), kallidin (Lys-BK), as well as their metabolites. The biological actions of these polypeptides binding on their receptors (B1 and B2) have been related to inflammation process, cytokines action, glutamate release and prostaglandins production. Usually, kinin B1 receptor is not expressed at a significant level under physiologic conditions in most tissues, but its expression is induced by injury, or upon exposure in vivo or in vitro to pro-inflammatory mediators. The kinin B2 receptor subtype is constitutively and widely expressed throughout the central and peripheral nervous system. These data raise the possibility for de novo expression of those receptors during the temporal lobe epilepsy (TLE), which has been related to cell death, gliosis and hippocampal reorganization. To correlate kinin system and TLE, adult male Wistar rats were submitted to pilocarpine model of epilepsy. The hippocampi were removed 6 h, 5 and 60 days after status epilepticus (SE) onset. The collected tissues were used to study the expression of kinin B1 and B2 mRNA receptors, using Real-Time PCR. Immunohistochemistry assay was also employed to visualize kinin B1 and B2 distribution in the hippocampus. The results show increased kinin B1 and B2 mRNA levels during acute, silent and chronic periods and changes in the kinin B1 and B2 receptors distribution. In addition, the immunoreactivity against kinin B1 receptor was increased mainly during the silent period, where neuron clusters of could be visualized. The kinin B2 receptor immunoreactivity also showed augmentation but mainly during the acute and silent periods. Our results suggest that kinin B1 and B2 receptors play an important role in the epileptic phenomena.

Authors+Show Affiliations

Departamento de Neurologia/Neurocirurgia, Universidade Federal de São Paulo, São Paulo, Brazil.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15047030

Citation

Argañaraz, Gustavo Adolfo, et al. "The Synthesis and Distribution of the Kinin B1 and B2 Receptors Are Modified in the Hippocampus of Rats Submitted to Pilocarpine Model of Epilepsy." Brain Research, vol. 1006, no. 1, 2004, pp. 114-25.
Argañaraz GA, Silva JA, Perosa SR, et al. The synthesis and distribution of the kinin B1 and B2 receptors are modified in the hippocampus of rats submitted to pilocarpine model of epilepsy. Brain Res. 2004;1006(1):114-25.
Argañaraz, G. A., Silva, J. A., Perosa, S. R., Pessoa, L. G., Carvalho, F. F., Bascands, J. L., Bader, M., da Silva Trindade, E., Amado, D., Cavalheiro, E. A., Pesquero, J. B., & da Graça Naffah-Mazzacoratti, M. (2004). The synthesis and distribution of the kinin B1 and B2 receptors are modified in the hippocampus of rats submitted to pilocarpine model of epilepsy. Brain Research, 1006(1), 114-25.
Argañaraz GA, et al. The Synthesis and Distribution of the Kinin B1 and B2 Receptors Are Modified in the Hippocampus of Rats Submitted to Pilocarpine Model of Epilepsy. Brain Res. 2004 Apr 23;1006(1):114-25. PubMed PMID: 15047030.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The synthesis and distribution of the kinin B1 and B2 receptors are modified in the hippocampus of rats submitted to pilocarpine model of epilepsy. AU - Argañaraz,Gustavo Adolfo, AU - Silva,José Antônio,Jr AU - Perosa,Sandra Regina, AU - Pessoa,Luciana Gilbert, AU - Carvalho,Fátima Ferreira, AU - Bascands,Jean Loup, AU - Bader,Michael, AU - da Silva Trindade,Edivaldo, AU - Amado,Débora, AU - Cavalheiro,Esper Abrão, AU - Pesquero,João Bosco, AU - da Graça Naffah-Mazzacoratti,Maria, PY - 2003/12/28/accepted PY - 2004/3/30/pubmed PY - 2004/6/21/medline PY - 2004/3/30/entrez SP - 114 EP - 25 JF - Brain research JO - Brain Res. VL - 1006 IS - 1 N2 - Kinins, a special class of polypeptides, are represented by bradykinin (BK), kallidin (Lys-BK), as well as their metabolites. The biological actions of these polypeptides binding on their receptors (B1 and B2) have been related to inflammation process, cytokines action, glutamate release and prostaglandins production. Usually, kinin B1 receptor is not expressed at a significant level under physiologic conditions in most tissues, but its expression is induced by injury, or upon exposure in vivo or in vitro to pro-inflammatory mediators. The kinin B2 receptor subtype is constitutively and widely expressed throughout the central and peripheral nervous system. These data raise the possibility for de novo expression of those receptors during the temporal lobe epilepsy (TLE), which has been related to cell death, gliosis and hippocampal reorganization. To correlate kinin system and TLE, adult male Wistar rats were submitted to pilocarpine model of epilepsy. The hippocampi were removed 6 h, 5 and 60 days after status epilepticus (SE) onset. The collected tissues were used to study the expression of kinin B1 and B2 mRNA receptors, using Real-Time PCR. Immunohistochemistry assay was also employed to visualize kinin B1 and B2 distribution in the hippocampus. The results show increased kinin B1 and B2 mRNA levels during acute, silent and chronic periods and changes in the kinin B1 and B2 receptors distribution. In addition, the immunoreactivity against kinin B1 receptor was increased mainly during the silent period, where neuron clusters of could be visualized. The kinin B2 receptor immunoreactivity also showed augmentation but mainly during the acute and silent periods. Our results suggest that kinin B1 and B2 receptors play an important role in the epileptic phenomena. SN - 0006-8993 UR - https://www.unboundmedicine.com/medline/citation/15047030/The_synthesis_and_distribution_of_the_kinin_B1_and_B2_receptors_are_modified_in_the_hippocampus_of_rats_submitted_to_pilocarpine_model_of_epilepsy_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0006899304002124 DB - PRIME DP - Unbound Medicine ER -