Tags

Type your tag names separated by a space and hit enter

Cre recombinase-mediated gene targeting of mesenchymal cells.
Genesis. 2004 Mar; 38(3):139-44.G

Abstract

Loss-of-function approaches by the Cre/loxP technology have provided powerful tools for functional analyses of genes of interest expressed preferentially in a particular tissue. Here we describe the generation of transgenic mouse lines expressing Cre recombinase under the control of the promoter/enhancer unit of the gene for the alpha2 chain of collagen type I (Col1alpha2). As an expression vector, we used a P1-derived artificial chromosome (PAC), which harbors approximately 100 kb carrying the col1alpha2 gene. The improved coding sequence of the Cre recombinase was introduced to replace the first exon of col1alpha2. Cre expression was determined by immunohistochemistry and Cre-mediated onset of beta-galactosidase expression in ROSA26R-Cre reporter mice. In four analyzed transgenic lines, Cre recombinase was efficiently expressed during embryogenesis and in adult animals in cells of mesenchymal origin, such as dermal fibroblasts, mesenchymal cells of blood vessel walls, and cells in fibrous connective tissues surrounding internal organs.

Authors+Show Affiliations

Division of Signal Transduction and Growth Control, Deutsches Krebsforschungszentrum, Heidelberg, Germany.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15048811

Citation

Florin, Lore, et al. "Cre Recombinase-mediated Gene Targeting of Mesenchymal Cells." Genesis (New York, N.Y. : 2000), vol. 38, no. 3, 2004, pp. 139-44.
Florin L, Alter H, Gröne HJ, et al. Cre recombinase-mediated gene targeting of mesenchymal cells. Genesis. 2004;38(3):139-44.
Florin, L., Alter, H., Gröne, H. J., Szabowski, A., Schütz, G., & Angel, P. (2004). Cre recombinase-mediated gene targeting of mesenchymal cells. Genesis (New York, N.Y. : 2000), 38(3), 139-44.
Florin L, et al. Cre Recombinase-mediated Gene Targeting of Mesenchymal Cells. Genesis. 2004;38(3):139-44. PubMed PMID: 15048811.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Cre recombinase-mediated gene targeting of mesenchymal cells. AU - Florin,Lore, AU - Alter,Heike, AU - Gröne,Hermann-Josef, AU - Szabowski,Axel, AU - Schütz,Günther, AU - Angel,Peter, PY - 2004/3/30/pubmed PY - 2004/10/27/medline PY - 2004/3/30/entrez SP - 139 EP - 44 JF - Genesis (New York, N.Y. : 2000) JO - Genesis VL - 38 IS - 3 N2 - Loss-of-function approaches by the Cre/loxP technology have provided powerful tools for functional analyses of genes of interest expressed preferentially in a particular tissue. Here we describe the generation of transgenic mouse lines expressing Cre recombinase under the control of the promoter/enhancer unit of the gene for the alpha2 chain of collagen type I (Col1alpha2). As an expression vector, we used a P1-derived artificial chromosome (PAC), which harbors approximately 100 kb carrying the col1alpha2 gene. The improved coding sequence of the Cre recombinase was introduced to replace the first exon of col1alpha2. Cre expression was determined by immunohistochemistry and Cre-mediated onset of beta-galactosidase expression in ROSA26R-Cre reporter mice. In four analyzed transgenic lines, Cre recombinase was efficiently expressed during embryogenesis and in adult animals in cells of mesenchymal origin, such as dermal fibroblasts, mesenchymal cells of blood vessel walls, and cells in fibrous connective tissues surrounding internal organs. SN - 1526-954X UR - https://www.unboundmedicine.com/medline/citation/15048811/Cre_recombinase_mediated_gene_targeting_of_mesenchymal_cells_ L2 - https://doi.org/10.1002/gene.20004 DB - PRIME DP - Unbound Medicine ER -