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Wheat germ lectin induces G2/M arrest in mouse L929 fibroblasts.
J Cell Biochem. 2004 Apr 15; 91(6):1159-73.JC

Abstract

Wheat germ lectin (WGA) is a cytotoxic lectin for many cell lines [Wang et al., 2000], but its underlying mechanism is not clear. In this report, we found that incubation of synchronized mouse L929 fibroblasts with WGA resulted in a dose-dependent reduction of intracellular incorporation of 3H-thymidine and MTT (3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide)-conversion activity (IC50 congruent with 0.4 microM). Fluorescein-conjugated WGA was demonstrated to transport from the cell surface into the paranuclear region of cultured L929 cells within 30 min, and subsequently evoked lipid peroxidation of plasma membrane and vacuolation in the cytoplasm of these cells. Studies with tritiated thymidine incorporation, immunofluorescence microscopy, immunoblotting analysis and flow cytometry revealed that WGA inhibited cell cycle progression after one replication, resulting in G2/M arrest and alteration of cell cycle regulatory proteins, particularly activation of p21Cip1/WAF1 and suppression of cyclin B and cdc 2. Although there was an increase of cytosolic caspase 3 and bax protein expression, no apoptotic bodies were observed by both fluorescence and transmission electron microscopy. These results suggest that WGA arrested L929 proliferation after one cell cycle in the G2/M phase through activation of the p21Cip1/WAF1 and suppression of Cyclin B-Cdc2.

Authors+Show Affiliations

Department of Anatomy, Faculty of Medicine, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong. ken-liu@cuhk.edu.hkNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15048871

Citation

Liu, W K., et al. "Wheat Germ Lectin Induces G2/M Arrest in Mouse L929 Fibroblasts." Journal of Cellular Biochemistry, vol. 91, no. 6, 2004, pp. 1159-73.
Liu WK, Sze SC, Ho JC, et al. Wheat germ lectin induces G2/M arrest in mouse L929 fibroblasts. J Cell Biochem. 2004;91(6):1159-73.
Liu, W. K., Sze, S. C., Ho, J. C., Liu, B. P., & Yu, M. C. (2004). Wheat germ lectin induces G2/M arrest in mouse L929 fibroblasts. Journal of Cellular Biochemistry, 91(6), 1159-73.
Liu WK, et al. Wheat Germ Lectin Induces G2/M Arrest in Mouse L929 Fibroblasts. J Cell Biochem. 2004 Apr 15;91(6):1159-73. PubMed PMID: 15048871.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Wheat germ lectin induces G2/M arrest in mouse L929 fibroblasts. AU - Liu,W K, AU - Sze,S C W, AU - Ho,J C K, AU - Liu,B P L, AU - Yu,M C, PY - 2004/3/30/pubmed PY - 2004/11/13/medline PY - 2004/3/30/entrez SP - 1159 EP - 73 JF - Journal of cellular biochemistry JO - J. Cell. Biochem. VL - 91 IS - 6 N2 - Wheat germ lectin (WGA) is a cytotoxic lectin for many cell lines [Wang et al., 2000], but its underlying mechanism is not clear. In this report, we found that incubation of synchronized mouse L929 fibroblasts with WGA resulted in a dose-dependent reduction of intracellular incorporation of 3H-thymidine and MTT (3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide)-conversion activity (IC50 congruent with 0.4 microM). Fluorescein-conjugated WGA was demonstrated to transport from the cell surface into the paranuclear region of cultured L929 cells within 30 min, and subsequently evoked lipid peroxidation of plasma membrane and vacuolation in the cytoplasm of these cells. Studies with tritiated thymidine incorporation, immunofluorescence microscopy, immunoblotting analysis and flow cytometry revealed that WGA inhibited cell cycle progression after one replication, resulting in G2/M arrest and alteration of cell cycle regulatory proteins, particularly activation of p21Cip1/WAF1 and suppression of cyclin B and cdc 2. Although there was an increase of cytosolic caspase 3 and bax protein expression, no apoptotic bodies were observed by both fluorescence and transmission electron microscopy. These results suggest that WGA arrested L929 proliferation after one cell cycle in the G2/M phase through activation of the p21Cip1/WAF1 and suppression of Cyclin B-Cdc2. SN - 0730-2312 UR - https://www.unboundmedicine.com/medline/citation/15048871/Wheat_germ_lectin_induces_G2/M_arrest_in_mouse_L929_fibroblasts_ L2 - https://doi.org/10.1002/jcb.10755 DB - PRIME DP - Unbound Medicine ER -