Tags

Type your tag names separated by a space and hit enter

L-Arginine ameliorates oxidative stress in alloxan-induced experimental diabetes mellitus.
J Appl Toxicol. 2004 Mar-Apr; 24(2):93-7.JA

Abstract

Oxidative stress occurs in diabetic patients and experimental models of diabetes. The ability of l-arginine to ameliorate the oxidative stress and metabolic changes after treatment with alloxan was investigated in rats. Adult male rats were injected intraperitoneally with 100 mg kg(-1) of alloxan to produce experimental oxidative stress characteristic of diabetes mellitus. Hyperglycaemia and hypercholesterolaemia were observed in serum after 7 days of alloxan treatment. This was associated with a depression of glutathione (GSH) concentration as well as superoxide dismutase (SOD) and catalase (CAT) activities in the liver and brain. In addition, the thiobarbituric acid-reactive substances (TBARS) were significantly elevated, indicating increased lipid peroxidation and oxidative stress in the same tissues. Administration of 100 mg kg(-1) l-arginine for 7 days either before or after alloxan injection significantly ameliorated the oxidative stress evidenced by a lower TBARS and a higher level of the endogenous GSH concentration and SOD and CAT activities than alloxan-treated rats. These effects were paralleled by marked protection and partial prophylaxis against alloxan-induced hyperglycaemia and cholesterolaemia. Thus, these results showed that exogenously administered l-arginine might improve the clinical manifestation of diabetes mellitus and decrease the oxidative stress in the liver and brain. In addition, the study supports the beneficial effect of l-arginine, which might be attributed to its direct, NO-dependent antioxidant capacity and/or NO-independent pathways.

Authors+Show Affiliations

Zoology Department, Faculty of Sciences, Mansoura University, Mansoura, A. R. Egypt. maelmissiry@yahoo.comNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15052603

Citation

El-Missiry, M A., et al. "L-Arginine Ameliorates Oxidative Stress in Alloxan-induced Experimental Diabetes Mellitus." Journal of Applied Toxicology : JAT, vol. 24, no. 2, 2004, pp. 93-7.
El-Missiry MA, Othman AI, Amer MA. L-Arginine ameliorates oxidative stress in alloxan-induced experimental diabetes mellitus. J Appl Toxicol. 2004;24(2):93-7.
El-Missiry, M. A., Othman, A. I., & Amer, M. A. (2004). L-Arginine ameliorates oxidative stress in alloxan-induced experimental diabetes mellitus. Journal of Applied Toxicology : JAT, 24(2), 93-7.
El-Missiry MA, Othman AI, Amer MA. L-Arginine Ameliorates Oxidative Stress in Alloxan-induced Experimental Diabetes Mellitus. J Appl Toxicol. 2004 Mar-Apr;24(2):93-7. PubMed PMID: 15052603.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - L-Arginine ameliorates oxidative stress in alloxan-induced experimental diabetes mellitus. AU - El-Missiry,M A, AU - Othman,A I, AU - Amer,M A, PY - 2004/3/31/pubmed PY - 2004/10/16/medline PY - 2004/3/31/entrez SP - 93 EP - 7 JF - Journal of applied toxicology : JAT JO - J Appl Toxicol VL - 24 IS - 2 N2 - Oxidative stress occurs in diabetic patients and experimental models of diabetes. The ability of l-arginine to ameliorate the oxidative stress and metabolic changes after treatment with alloxan was investigated in rats. Adult male rats were injected intraperitoneally with 100 mg kg(-1) of alloxan to produce experimental oxidative stress characteristic of diabetes mellitus. Hyperglycaemia and hypercholesterolaemia were observed in serum after 7 days of alloxan treatment. This was associated with a depression of glutathione (GSH) concentration as well as superoxide dismutase (SOD) and catalase (CAT) activities in the liver and brain. In addition, the thiobarbituric acid-reactive substances (TBARS) were significantly elevated, indicating increased lipid peroxidation and oxidative stress in the same tissues. Administration of 100 mg kg(-1) l-arginine for 7 days either before or after alloxan injection significantly ameliorated the oxidative stress evidenced by a lower TBARS and a higher level of the endogenous GSH concentration and SOD and CAT activities than alloxan-treated rats. These effects were paralleled by marked protection and partial prophylaxis against alloxan-induced hyperglycaemia and cholesterolaemia. Thus, these results showed that exogenously administered l-arginine might improve the clinical manifestation of diabetes mellitus and decrease the oxidative stress in the liver and brain. In addition, the study supports the beneficial effect of l-arginine, which might be attributed to its direct, NO-dependent antioxidant capacity and/or NO-independent pathways. SN - 0260-437X UR - https://www.unboundmedicine.com/medline/citation/15052603/L_Arginine_ameliorates_oxidative_stress_in_alloxan_induced_experimental_diabetes_mellitus_ L2 - https://doi.org/10.1002/jat.952 DB - PRIME DP - Unbound Medicine ER -