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Analysis of coffee for the presence of acrylamide by LC-MS/MS.
J Agric Food Chem. 2004 Apr 07; 52(7):1996-2002.JA

Abstract

A variety of popular instant, ground, and brewed coffees were analyzed using a modified liquid chromatography-tandem mass spectrometry (LC-MS/MS) method specifically developed for the determination of acrylamide in foods. Coffee test portions were spiked with 13C3-labeled acrylamide as an internal standard prior to their extraction and cleanup. Ground coffees (1 g) and instant coffees (0.5 g) were extracted by shaking with 9 mL of water for 20 min. Brewed coffee test portions (9 mL) were taken through the cleanup procedure without further dilution with extraction solvent. Coffee test portions were cleaned up by passing 1.5 mL first through an Oasis HLB (hydrophilic/lipophilic copolymer sorbent) solid phase extraction (SPE) cartridge and then a Bond Elut-Accucat (cation and anion exchange sorbent) SPE cartridge. The cleaned up extracts were analyzed by positive ion electrospray LC-MS/MS. The MS/MS data was used to detect, confirm, and quantitate acrylamide. The limit of quantitation of the method was 10 ng/g for ground and instant coffees and 1.0 ng/mL for brewed coffee. The levels of acrylamide ranged from 45 to 374 ng/g in unbrewed coffee grounds, from 172 to 539 ng/g in instant coffee crystals, and from 6 to 16 ng/mL in brewed coffee.

Authors+Show Affiliations

Center for Food Safety and Applied Nutrition, Instrumentation and Biophysics Branch, U.S. Food and Drug Administration, HFS-717, 5100 Paint Branch Parkway, College Park, Maryland 20740, USA. dandrzej@cfsan.fda.govNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

15053542

Citation

Andrzejewski, Denis, et al. "Analysis of Coffee for the Presence of Acrylamide By LC-MS/MS." Journal of Agricultural and Food Chemistry, vol. 52, no. 7, 2004, pp. 1996-2002.
Andrzejewski D, Roach JA, Gay ML, et al. Analysis of coffee for the presence of acrylamide by LC-MS/MS. J Agric Food Chem. 2004;52(7):1996-2002.
Andrzejewski, D., Roach, J. A., Gay, M. L., & Musser, S. M. (2004). Analysis of coffee for the presence of acrylamide by LC-MS/MS. Journal of Agricultural and Food Chemistry, 52(7), 1996-2002.
Andrzejewski D, et al. Analysis of Coffee for the Presence of Acrylamide By LC-MS/MS. J Agric Food Chem. 2004 Apr 7;52(7):1996-2002. PubMed PMID: 15053542.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Analysis of coffee for the presence of acrylamide by LC-MS/MS. AU - Andrzejewski,Denis, AU - Roach,John A G, AU - Gay,Martha L, AU - Musser,Steven M, PY - 2004/4/1/pubmed PY - 2004/5/8/medline PY - 2004/4/1/entrez SP - 1996 EP - 2002 JF - Journal of agricultural and food chemistry JO - J Agric Food Chem VL - 52 IS - 7 N2 - A variety of popular instant, ground, and brewed coffees were analyzed using a modified liquid chromatography-tandem mass spectrometry (LC-MS/MS) method specifically developed for the determination of acrylamide in foods. Coffee test portions were spiked with 13C3-labeled acrylamide as an internal standard prior to their extraction and cleanup. Ground coffees (1 g) and instant coffees (0.5 g) were extracted by shaking with 9 mL of water for 20 min. Brewed coffee test portions (9 mL) were taken through the cleanup procedure without further dilution with extraction solvent. Coffee test portions were cleaned up by passing 1.5 mL first through an Oasis HLB (hydrophilic/lipophilic copolymer sorbent) solid phase extraction (SPE) cartridge and then a Bond Elut-Accucat (cation and anion exchange sorbent) SPE cartridge. The cleaned up extracts were analyzed by positive ion electrospray LC-MS/MS. The MS/MS data was used to detect, confirm, and quantitate acrylamide. The limit of quantitation of the method was 10 ng/g for ground and instant coffees and 1.0 ng/mL for brewed coffee. The levels of acrylamide ranged from 45 to 374 ng/g in unbrewed coffee grounds, from 172 to 539 ng/g in instant coffee crystals, and from 6 to 16 ng/mL in brewed coffee. SN - 0021-8561 UR - https://www.unboundmedicine.com/medline/citation/15053542/Analysis_of_coffee_for_the_presence_of_acrylamide_by_LC_MS/MS_ DB - PRIME DP - Unbound Medicine ER -