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The metabolism of the piperazine-type phenothiazine neuroleptic perazine by the human cytochrome P-450 isoenzymes.
Eur Neuropsychopharmacol. 2004 May; 14(3):199-208.EN

Abstract

Identification of cytochrome P-450 isoenzymes (CYPs) involved in perazine 5-sulphoxidation and N-demethylation was carried out using human liver microsomes and cDNA-expressed human CYPs (Supersomes). In human liver microsomes, the formation of perazine metabolites correlated significantly with the level of CYP1A2 and ethoxyrezorufin O-deethylase activity, as well as with the level of CYP3A4 and cyclosporin A oxidase activity. Moreover, the formation of N-desmethylperazine also correlated well with S-mephenytoin 4'-hydroxylase activity (CYP2C19). alpha-Naphthoflavone (a CYP1A2 inhibitor) and ketoconazole (a CYP3A4 inhibitor) significantly decreased the rate of perazine 5-sulphoxidation, while ticlopidine (a CYP2C19 inhibitor) strongly reduced the rate of perazine N-demethylation in human liver microsomes. The cDNA-expressed human CYPs generated different amounts of perazine metabolites, but the preference of CYP isoforms to catalyze perazine metabolism was as follows (pmol of product/pmol of CYP isoform/min): 1A1>2D6>2C19>1A2>2B6>2E1>2A6 approximately 3A4>2C9 for 5-sulphoxidation and 2C19>2D6>1A1>1A2>2B6>3A4>2C9>2A6 for N-demethylation. In the light of the obtained results and regarding the contribution of each isoform to the total amount of CYP in human liver, it is concluded that CYP1A2 and CYP3A4 are the main isoenzymes catalyzing 5-sulphoxidation (32% and 30%, respectively), while CYP2C19 is the main isoform catalyzing perazine N-demethylation (68%). CYP2C9, CYP2E1 CYP2C19 and CYP2D6 are engaged to a lesser degree in 5-sulphoxidation, while CYP1A2, CYP3A4 and CYP2D6 in perazine N-demethylation (6-10%, depending on the isoform).

Authors+Show Affiliations

Polish Academy of Sciences, Institute of Pharmacology, Smetna 12, 31-343, Cracow, Poland. wojcikow@if-pan.krakow.plNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15056479

Citation

Wójcikowski, Jacek, et al. "The Metabolism of the Piperazine-type Phenothiazine Neuroleptic Perazine By the Human Cytochrome P-450 Isoenzymes." European Neuropsychopharmacology : the Journal of the European College of Neuropsychopharmacology, vol. 14, no. 3, 2004, pp. 199-208.
Wójcikowski J, Pichard-Garcia L, Maurel P, et al. The metabolism of the piperazine-type phenothiazine neuroleptic perazine by the human cytochrome P-450 isoenzymes. Eur Neuropsychopharmacol. 2004;14(3):199-208.
Wójcikowski, J., Pichard-Garcia, L., Maurel, P., & Daniel, W. A. (2004). The metabolism of the piperazine-type phenothiazine neuroleptic perazine by the human cytochrome P-450 isoenzymes. European Neuropsychopharmacology : the Journal of the European College of Neuropsychopharmacology, 14(3), 199-208.
Wójcikowski J, et al. The Metabolism of the Piperazine-type Phenothiazine Neuroleptic Perazine By the Human Cytochrome P-450 Isoenzymes. Eur Neuropsychopharmacol. 2004;14(3):199-208. PubMed PMID: 15056479.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The metabolism of the piperazine-type phenothiazine neuroleptic perazine by the human cytochrome P-450 isoenzymes. AU - Wójcikowski,Jacek, AU - Pichard-Garcia,Lydiane, AU - Maurel,Patrick, AU - Daniel,Władysława A, PY - 2003/05/13/received PY - 2003/07/03/revised PY - 2003/07/08/accepted PY - 2004/4/2/pubmed PY - 2004/6/24/medline PY - 2004/4/2/entrez SP - 199 EP - 208 JF - European neuropsychopharmacology : the journal of the European College of Neuropsychopharmacology JO - Eur Neuropsychopharmacol VL - 14 IS - 3 N2 - Identification of cytochrome P-450 isoenzymes (CYPs) involved in perazine 5-sulphoxidation and N-demethylation was carried out using human liver microsomes and cDNA-expressed human CYPs (Supersomes). In human liver microsomes, the formation of perazine metabolites correlated significantly with the level of CYP1A2 and ethoxyrezorufin O-deethylase activity, as well as with the level of CYP3A4 and cyclosporin A oxidase activity. Moreover, the formation of N-desmethylperazine also correlated well with S-mephenytoin 4'-hydroxylase activity (CYP2C19). alpha-Naphthoflavone (a CYP1A2 inhibitor) and ketoconazole (a CYP3A4 inhibitor) significantly decreased the rate of perazine 5-sulphoxidation, while ticlopidine (a CYP2C19 inhibitor) strongly reduced the rate of perazine N-demethylation in human liver microsomes. The cDNA-expressed human CYPs generated different amounts of perazine metabolites, but the preference of CYP isoforms to catalyze perazine metabolism was as follows (pmol of product/pmol of CYP isoform/min): 1A1>2D6>2C19>1A2>2B6>2E1>2A6 approximately 3A4>2C9 for 5-sulphoxidation and 2C19>2D6>1A1>1A2>2B6>3A4>2C9>2A6 for N-demethylation. In the light of the obtained results and regarding the contribution of each isoform to the total amount of CYP in human liver, it is concluded that CYP1A2 and CYP3A4 are the main isoenzymes catalyzing 5-sulphoxidation (32% and 30%, respectively), while CYP2C19 is the main isoform catalyzing perazine N-demethylation (68%). CYP2C9, CYP2E1 CYP2C19 and CYP2D6 are engaged to a lesser degree in 5-sulphoxidation, while CYP1A2, CYP3A4 and CYP2D6 in perazine N-demethylation (6-10%, depending on the isoform). SN - 0924-977X UR - https://www.unboundmedicine.com/medline/citation/15056479/The_metabolism_of_the_piperazine_type_phenothiazine_neuroleptic_perazine_by_the_human_cytochrome_P_450_isoenzymes_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0924977X03001056 DB - PRIME DP - Unbound Medicine ER -