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Pseudomonas aeruginosa autoinducer enters and functions in mammalian cells.
J Bacteriol. 2004 Apr; 186(8):2281-7.JB

Abstract

Quorum sensing (QS) is a cell density-dependent signaling mechanism used by many bacteria to control gene expression. Several recent reports indicate that the signaling molecules (autoinducers) that mediate QS in Pseudomonas aeruginosa may also modulate gene expression in host cells; however, the mechanisms are largely unknown. Here we show that two P. aeruginosa autoinducers, N-3-oxododecanoyl-homoserine lactone and N-butyryl-homoserine lactone, can both enter eukaryotic cells and activate artificial chimeric transcription factors based on their cognate transcriptional activators, LasR and RhlR, respectively. The autoinducers promoted nuclear localization of chimeric proteins containing the full LasR or RhlR coding region, and the LasR-based proteins were capable of activating transcription of a LasR-dependent luciferase gene. Responsiveness to autoinducer required the N-terminal autoinducer-binding domains of LasR and RhlR. Truncated proteins consisting of only the C-terminal helix-turn-helix DNA-binding domains of both proteins attached to a nuclear localization signal efficiently translocated to the nucleus in the absence of autoinducer, and truncated LasR-based proteins functioned as constitutively active transcription factors. Chimeric LasR proteins were only activated by their cognate autoinducer ligand and not by N-butyryl-L-homoserine lactone. These data provide evidence that autoinducer molecules from human pathogens can enter mammalian cells and suggest that autoinducers may influence gene expression in host cells by interacting with and activating as-yet-unidentified endogenous proteins.

Authors+Show Affiliations

Department of Cell Biology and Biochemistry, Texas Tech University Health Sciences Center, Lubbock, Texas 79430, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

15060029

Citation

Williams, Simon C., et al. "Pseudomonas Aeruginosa Autoinducer Enters and Functions in Mammalian Cells." Journal of Bacteriology, vol. 186, no. 8, 2004, pp. 2281-7.
Williams SC, Patterson EK, Carty NL, et al. Pseudomonas aeruginosa autoinducer enters and functions in mammalian cells. J Bacteriol. 2004;186(8):2281-7.
Williams, S. C., Patterson, E. K., Carty, N. L., Griswold, J. A., Hamood, A. N., & Rumbaugh, K. P. (2004). Pseudomonas aeruginosa autoinducer enters and functions in mammalian cells. Journal of Bacteriology, 186(8), 2281-7.
Williams SC, et al. Pseudomonas Aeruginosa Autoinducer Enters and Functions in Mammalian Cells. J Bacteriol. 2004;186(8):2281-7. PubMed PMID: 15060029.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Pseudomonas aeruginosa autoinducer enters and functions in mammalian cells. AU - Williams,Simon C, AU - Patterson,Erin K, AU - Carty,Nancy L, AU - Griswold,John A, AU - Hamood,Abdul N, AU - Rumbaugh,Kendra P, PY - 2004/4/3/pubmed PY - 2004/5/25/medline PY - 2004/4/3/entrez SP - 2281 EP - 7 JF - Journal of bacteriology JO - J. Bacteriol. VL - 186 IS - 8 N2 - Quorum sensing (QS) is a cell density-dependent signaling mechanism used by many bacteria to control gene expression. Several recent reports indicate that the signaling molecules (autoinducers) that mediate QS in Pseudomonas aeruginosa may also modulate gene expression in host cells; however, the mechanisms are largely unknown. Here we show that two P. aeruginosa autoinducers, N-3-oxododecanoyl-homoserine lactone and N-butyryl-homoserine lactone, can both enter eukaryotic cells and activate artificial chimeric transcription factors based on their cognate transcriptional activators, LasR and RhlR, respectively. The autoinducers promoted nuclear localization of chimeric proteins containing the full LasR or RhlR coding region, and the LasR-based proteins were capable of activating transcription of a LasR-dependent luciferase gene. Responsiveness to autoinducer required the N-terminal autoinducer-binding domains of LasR and RhlR. Truncated proteins consisting of only the C-terminal helix-turn-helix DNA-binding domains of both proteins attached to a nuclear localization signal efficiently translocated to the nucleus in the absence of autoinducer, and truncated LasR-based proteins functioned as constitutively active transcription factors. Chimeric LasR proteins were only activated by their cognate autoinducer ligand and not by N-butyryl-L-homoserine lactone. These data provide evidence that autoinducer molecules from human pathogens can enter mammalian cells and suggest that autoinducers may influence gene expression in host cells by interacting with and activating as-yet-unidentified endogenous proteins. SN - 0021-9193 UR - https://www.unboundmedicine.com/medline/citation/15060029/Pseudomonas_aeruginosa_autoinducer_enters_and_functions_in_mammalian_cells_ L2 - http://jb.asm.org/cgi/pmidlookup?view=long&pmid=15060029 DB - PRIME DP - Unbound Medicine ER -