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Analysis of phenolic compounds and radical scavenging activity of Echinacea spp.
J Pharm Biomed Anal. 2004 Apr 16; 35(2):289-301.JP

Abstract

The aim of this study was to set up and validate an RP-LC method with DAD-detection to quantify caffeic acid derivatives in various Echinacea spp. Samples were extracted with 80% methanol. The analyses were carried out on a Lichrospher RP-18 column (125 mm x 4 mm i.d., 5 microm), with a mobile phase gradient, which increases the acetonitrile level in a phosphoric acid solution (0.1%). The flow rate was 1.5 ml/min. Detection was set at 330 nm. This method allowed the identification and quantification of caftaric acid, chlorogenic acid, caffeic acid, cynarin, echinacoside and cichoric acid in Echinacea roots and derivatives. The total phenolic content was 10.49 mg/g for E. angustifolia, 17.83 mg/g for E. pallida and 23.23 mg/g for E. purpurea. Among Echinacea commercial herbal medicines, a certain variability in the concentrations of phenolic compounds was observed. The radical scavenging activity of Echinacea methanolic extracts was evaluated in vitro with a spectrophotometric method based on the reduction of an alcoholic 2,2-diphenyl-1-picrylhydrazyl (DPPH*) radical solution at 517 nm in the presence of a hydrogen donating antioxidant. As for pure compounds, echinacoside had the highest capacity to quench DPPH* radicals (EC50 = 6.6 microM), while caftaric acid had the lowest (EC50 = 20.5 microM). The average EC50 values for E. purpurea, E. pallida and E. angustifolia were 134, 167 and 231 microg/ml, respectively. The radical scavenging activity of Echinacea root extracts reflected their phenolic composition. The results indicate that Echinacea roots and derivatives are a good source of natural antioxidants and could be used to prevent free-radical-induced deleterious effects.

Authors+Show Affiliations

Department of Pharmaceutical Sciences, University of Modena and Reggio Emilia, Via G. Campi 183, 41100 Modena, Italy.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15063463

Citation

Pellati, Federica, et al. "Analysis of Phenolic Compounds and Radical Scavenging Activity of Echinacea Spp." Journal of Pharmaceutical and Biomedical Analysis, vol. 35, no. 2, 2004, pp. 289-301.
Pellati F, Benvenuti S, Magro L, et al. Analysis of phenolic compounds and radical scavenging activity of Echinacea spp. J Pharm Biomed Anal. 2004;35(2):289-301.
Pellati, F., Benvenuti, S., Magro, L., Melegari, M., & Soragni, F. (2004). Analysis of phenolic compounds and radical scavenging activity of Echinacea spp. Journal of Pharmaceutical and Biomedical Analysis, 35(2), 289-301.
Pellati F, et al. Analysis of Phenolic Compounds and Radical Scavenging Activity of Echinacea Spp. J Pharm Biomed Anal. 2004 Apr 16;35(2):289-301. PubMed PMID: 15063463.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Analysis of phenolic compounds and radical scavenging activity of Echinacea spp. AU - Pellati,Federica, AU - Benvenuti,Stefania, AU - Magro,Lara, AU - Melegari,Michele, AU - Soragni,Fabrizia, PY - 2003/07/04/received PY - 2003/09/12/revised PY - 2003/09/20/accepted PY - 2004/4/6/pubmed PY - 2004/12/16/medline PY - 2004/4/6/entrez SP - 289 EP - 301 JF - Journal of pharmaceutical and biomedical analysis JO - J Pharm Biomed Anal VL - 35 IS - 2 N2 - The aim of this study was to set up and validate an RP-LC method with DAD-detection to quantify caffeic acid derivatives in various Echinacea spp. Samples were extracted with 80% methanol. The analyses were carried out on a Lichrospher RP-18 column (125 mm x 4 mm i.d., 5 microm), with a mobile phase gradient, which increases the acetonitrile level in a phosphoric acid solution (0.1%). The flow rate was 1.5 ml/min. Detection was set at 330 nm. This method allowed the identification and quantification of caftaric acid, chlorogenic acid, caffeic acid, cynarin, echinacoside and cichoric acid in Echinacea roots and derivatives. The total phenolic content was 10.49 mg/g for E. angustifolia, 17.83 mg/g for E. pallida and 23.23 mg/g for E. purpurea. Among Echinacea commercial herbal medicines, a certain variability in the concentrations of phenolic compounds was observed. The radical scavenging activity of Echinacea methanolic extracts was evaluated in vitro with a spectrophotometric method based on the reduction of an alcoholic 2,2-diphenyl-1-picrylhydrazyl (DPPH*) radical solution at 517 nm in the presence of a hydrogen donating antioxidant. As for pure compounds, echinacoside had the highest capacity to quench DPPH* radicals (EC50 = 6.6 microM), while caftaric acid had the lowest (EC50 = 20.5 microM). The average EC50 values for E. purpurea, E. pallida and E. angustifolia were 134, 167 and 231 microg/ml, respectively. The radical scavenging activity of Echinacea root extracts reflected their phenolic composition. The results indicate that Echinacea roots and derivatives are a good source of natural antioxidants and could be used to prevent free-radical-induced deleterious effects. SN - 0731-7085 UR - https://www.unboundmedicine.com/medline/citation/15063463/Analysis_of_phenolic_compounds_and_radical_scavenging_activity_of_Echinacea_spp_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0731708503006459 DB - PRIME DP - Unbound Medicine ER -