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Engineered autolytic yeast strains secreting Kluyveromyces lactis beta-galactosidase for production of heterologous proteins in lactose media.
J Biotechnol. 2004 Apr 08; 109(1-2):131-7.JB

Abstract

Secretion of the heterologous Kluyveromyces lactis beta-galactosidase into culture medium by several Saccharomyces cerevisiae osmotic-remedial thermosensitive-autolytic mutants was assayed and proved that new metabolic abilities were conferred since the constructed strains were able to grow in lactose-containing media. Cell growth became independent of a lactose-uptake mechanism. Higher levels of extra-cellular and intra-cellular beta-galactosidase production, lactose consumption and growth were obtained with the LHDP1 strain, showing a thermosensitive-autolytic phenotype as well as being peptidase-defective. The recombinant strain LHDP1 presented the highest beta-galactosidase yields from biomass and the lowest ethanol levels from lactose. This strain is effective for the heterologous production and release of K. lactis beta-galactosidase into the extra-cellular medium after osmotic shock.

Authors+Show Affiliations

Becerra M
Departamento de Bioloxía Celular e Molecular, Facultade de Ciencias, Universidade da Coruña, Campus da Zapateira s/n, 15071-A Coruña, Spain. manu@udc.es
Rodríguez-Belmonte E
No affiliation info available
Esperanza Cerdán M
No affiliation info available
González Siso MI
No affiliation info available

MeSH

CheeseEthanolGenetic EngineeringKluyveromycesLactoseOsmosisRecombinant ProteinsSaccharomyces cerevisiaebeta-Galactosidase

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15063621

Citation

Becerra, Manuel, et al. "Engineered Autolytic Yeast Strains Secreting Kluyveromyces Lactis Beta-galactosidase for Production of Heterologous Proteins in Lactose Media." Journal of Biotechnology, vol. 109, no. 1-2, 2004, pp. 131-7.
Becerra M, Rodríguez-Belmonte E, Esperanza Cerdán M, et al. Engineered autolytic yeast strains secreting Kluyveromyces lactis beta-galactosidase for production of heterologous proteins in lactose media. J Biotechnol. 2004;109(1-2):131-7.
Becerra, M., Rodríguez-Belmonte, E., Esperanza Cerdán, M., & González Siso, M. I. (2004). Engineered autolytic yeast strains secreting Kluyveromyces lactis beta-galactosidase for production of heterologous proteins in lactose media. Journal of Biotechnology, 109(1-2), 131-7.
Becerra M, et al. Engineered Autolytic Yeast Strains Secreting Kluyveromyces Lactis Beta-galactosidase for Production of Heterologous Proteins in Lactose Media. J Biotechnol. 2004 Apr 8;109(1-2):131-7. PubMed PMID: 15063621.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Engineered autolytic yeast strains secreting Kluyveromyces lactis beta-galactosidase for production of heterologous proteins in lactose media. AU - Becerra,Manuel, AU - Rodríguez-Belmonte,Esther, AU - Esperanza Cerdán,M, AU - González Siso,M Isabel, PY - 2002/11/16/received PY - 2003/06/02/revised PY - 2003/10/14/accepted PY - 2004/4/6/pubmed PY - 2004/12/16/medline PY - 2004/4/6/entrez SP - 131 EP - 7 JF - Journal of biotechnology JO - J Biotechnol VL - 109 IS - 1-2 N2 - Secretion of the heterologous Kluyveromyces lactis beta-galactosidase into culture medium by several Saccharomyces cerevisiae osmotic-remedial thermosensitive-autolytic mutants was assayed and proved that new metabolic abilities were conferred since the constructed strains were able to grow in lactose-containing media. Cell growth became independent of a lactose-uptake mechanism. Higher levels of extra-cellular and intra-cellular beta-galactosidase production, lactose consumption and growth were obtained with the LHDP1 strain, showing a thermosensitive-autolytic phenotype as well as being peptidase-defective. The recombinant strain LHDP1 presented the highest beta-galactosidase yields from biomass and the lowest ethanol levels from lactose. This strain is effective for the heterologous production and release of K. lactis beta-galactosidase into the extra-cellular medium after osmotic shock. SN - 0168-1656 UR - https://www.unboundmedicine.com/medline/citation/15063621/Engineered_autolytic_yeast_strains_secreting_Kluyveromyces_lactis_beta_galactosidase_for_production_of_heterologous_proteins_in_lactose_media_ DB - PRIME DP - Unbound Medicine ER -