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[Influence of different mechanical environments on repair of cartilage defect with rabbit marrow mesenchymal stem cells].
Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi. 2004 Mar; 18(2):96-9.ZX

Abstract

OBJECTIVE

To study the influence of different mechanical environments on repair cartilage defect with marrow mesenchymal stem cells as seed cells.

METHODS

The rabbit marrow mesenchymal stem cells were isolated and cultured. The cartilage defects were repaired by autologous tissue engineered cartilage with the marrow mesenchymal stem cells as seed cells. Fifteen rabbits with cartilage defect were divided into 3 groups: dislocation group with cell-free scaffold (control group), dislocation group with cartilaginous construct and normal mechanical environment group with cartilaginous construct. The repaired tissue was harvested and examined 6 weeks postoperatively.

RESULTS

The repair tissue in normal mechanical environment group with cartilaginous construct showed cartilage-like tissue in superficial layer and subchondral bone tissue in deep layer 6 weeks postoperatively. The defect was filled with bone tissue in dislocation group with cartilaginous construct 6 weeks postoperatively. The surrounding normal cartilage tissue showed vascular invasion from subchondral area and the concomitant thinning of the normal cartilage layer. The cartilaginous construct left in the femoral trochlea groove formed hyaline cartilage-like tissue. The defect was repaired by fibrous tissue in control group.

CONCLUSION

The repaired tissue by tissue engineered cartilage with marrow mesenchymal stem cells as seed cells showed the best result in normal mechanical environment group, which indicates that it will be essential for the formation and maintenance of tissue engineered cartilage to keep the normal mechanical stress stimulus.

Authors+Show Affiliations

Wang G
Department of Orthopedic Surgery, First Teaching Hospital of Jilin University, Changchun, Jilin, P. R. China 130021.
Liu Y
No affiliation info available
Shan YX
No affiliation info available

MeSH

AnimalsCartilage, ArticularCells, CulturedChondrogenesisFemaleKnee JointMaleMesenchymal Stem Cell TransplantationProstheses and ImplantsRabbitsStress, MechanicalTissue EngineeringTransplantation, Autologous

Pub Type(s)

English Abstract
Journal Article
Research Support, Non-U.S. Gov't

Language

chi

PubMed ID

15065405

Citation

Wang, Gang, et al. "[Influence of Different Mechanical Environments On Repair of Cartilage Defect With Rabbit Marrow Mesenchymal Stem Cells]." Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi = Zhongguo Xiufu Chongjian Waike Zazhi = Chinese Journal of Reparative and Reconstructive Surgery, vol. 18, no. 2, 2004, pp. 96-9.
Wang G, Liu Y, Shan YX. [Influence of different mechanical environments on repair of cartilage defect with rabbit marrow mesenchymal stem cells]. Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi. 2004;18(2):96-9.
Wang, G., Liu, Y., & Shan, Y. X. (2004). [Influence of different mechanical environments on repair of cartilage defect with rabbit marrow mesenchymal stem cells]. Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi = Zhongguo Xiufu Chongjian Waike Zazhi = Chinese Journal of Reparative and Reconstructive Surgery, 18(2), 96-9.
Wang G, Liu Y, Shan YX. [Influence of Different Mechanical Environments On Repair of Cartilage Defect With Rabbit Marrow Mesenchymal Stem Cells]. Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi. 2004;18(2):96-9. PubMed PMID: 15065405.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Influence of different mechanical environments on repair of cartilage defect with rabbit marrow mesenchymal stem cells]. AU - Wang,Gang, AU - Liu,Yi, AU - Shan,Yu-xing, PY - 2004/4/7/pubmed PY - 2004/9/8/medline PY - 2004/4/7/entrez SP - 96 EP - 9 JF - Zhongguo xiu fu chong jian wai ke za zhi = Zhongguo xiufu chongjian waike zazhi = Chinese journal of reparative and reconstructive surgery JO - Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi VL - 18 IS - 2 N2 - OBJECTIVE: To study the influence of different mechanical environments on repair cartilage defect with marrow mesenchymal stem cells as seed cells. METHODS: The rabbit marrow mesenchymal stem cells were isolated and cultured. The cartilage defects were repaired by autologous tissue engineered cartilage with the marrow mesenchymal stem cells as seed cells. Fifteen rabbits with cartilage defect were divided into 3 groups: dislocation group with cell-free scaffold (control group), dislocation group with cartilaginous construct and normal mechanical environment group with cartilaginous construct. The repaired tissue was harvested and examined 6 weeks postoperatively. RESULTS: The repair tissue in normal mechanical environment group with cartilaginous construct showed cartilage-like tissue in superficial layer and subchondral bone tissue in deep layer 6 weeks postoperatively. The defect was filled with bone tissue in dislocation group with cartilaginous construct 6 weeks postoperatively. The surrounding normal cartilage tissue showed vascular invasion from subchondral area and the concomitant thinning of the normal cartilage layer. The cartilaginous construct left in the femoral trochlea groove formed hyaline cartilage-like tissue. The defect was repaired by fibrous tissue in control group. CONCLUSION: The repaired tissue by tissue engineered cartilage with marrow mesenchymal stem cells as seed cells showed the best result in normal mechanical environment group, which indicates that it will be essential for the formation and maintenance of tissue engineered cartilage to keep the normal mechanical stress stimulus. SN - 1002-1892 UR - https://www.unboundmedicine.com/medline/citation/15065405/[Influence_of_different_mechanical_environments_on_repair_of_cartilage_defect_with_rabbit_marrow_mesenchymal_stem_cells]_ DB - PRIME DP - Unbound Medicine ER -