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The influence of Lactobacillus brevis on ornithine decarboxylase activity and polyamine profiles in Helicobacter pylori-infected gastric mucosa.
Helicobacter 2004; 9(2):165-72H

Abstract

BACKGROUND

Functional probiotics may prevent Helicobacter pylori infection, and some evidence suggests that they also possess antitumor properties. Lactobacillus brevis (CD2) is a functional Lactobacillus strain with peculiar biochemical features, essentially related to the activity of arginine deiminase. This enzyme catalyzes the catabolism of arginine and affects the biosynthesis of polyamines (putrescine, spermidine, and spermine). Polyamines are polycations found in high concentrations in both normal and neoplastic cells. Our aims were: 1, to assess whether oral administration of L. brevis (CD2) affects H. pylori survival in the human gastric mucosa; 2, to evaluate the effects of L. brevis (CD2) on polyamine biosynthesis in gastric biopsies from H. pylori-positive patients.

MATERIALS AND METHODS

For 3 weeks before endoscopy, 22 H. pylori-positive dyspeptic patients randomly received (ratio 1 : 1) high oral doses of L. brevis (CD2) or placebo. Before and after treatment, H. pylori infection was determined by urea breath test (UBT). In gastric biopsies, ornithine decarboxylase activity and polyamine levels were, respectively, evaluated by a radiometric technique and high-pressure liquid chromatography (HPLC).

RESULTS

L. brevis (CD2) treatment did not eradicate H. pylori. However, a reduction in the UBT delta values occurred, suggesting a decrease in intragastric bacterial load. Significantly, L. brevis (CD2) induced a decrease in gastric ornithine decarboxylase activity and polyamine levels.

CONCLUSIONS

Our data support the hypothesis that L. brevis (CD2) treatment decreases H. pylori colonization, thus reducing polyamine biosynthesis. Alternatively, the arginine deiminase activity following L. brevis (CD2) administration might cause arginine deficiency, preventing polyamine generation from gastric cells.

Authors+Show Affiliations

Laboratory of Biochemistry, Scientific Institute for Digestive Diseases IRCCS 'Saverio de Bellis', Bari, Italy.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Clinical Trial
Journal Article
Randomized Controlled Trial

Language

eng

PubMed ID

15068419

Citation

Linsalata, Michele, et al. "The Influence of Lactobacillus Brevis On Ornithine Decarboxylase Activity and Polyamine Profiles in Helicobacter Pylori-infected Gastric Mucosa." Helicobacter, vol. 9, no. 2, 2004, pp. 165-72.
Linsalata M, Russo F, Berloco P, et al. The influence of Lactobacillus brevis on ornithine decarboxylase activity and polyamine profiles in Helicobacter pylori-infected gastric mucosa. Helicobacter. 2004;9(2):165-72.
Linsalata, M., Russo, F., Berloco, P., Caruso, M. L., Matteo, G. D., Cifone, M. G., ... Di Leo, A. (2004). The influence of Lactobacillus brevis on ornithine decarboxylase activity and polyamine profiles in Helicobacter pylori-infected gastric mucosa. Helicobacter, 9(2), pp. 165-72.
Linsalata M, et al. The Influence of Lactobacillus Brevis On Ornithine Decarboxylase Activity and Polyamine Profiles in Helicobacter Pylori-infected Gastric Mucosa. Helicobacter. 2004;9(2):165-72. PubMed PMID: 15068419.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The influence of Lactobacillus brevis on ornithine decarboxylase activity and polyamine profiles in Helicobacter pylori-infected gastric mucosa. AU - Linsalata,Michele, AU - Russo,Francesco, AU - Berloco,Pasquale, AU - Caruso,Maria Lucia, AU - Matteo,Giovanni D I, AU - Cifone,Maria Grazia, AU - Simone,Claudio D E, AU - Ierardi,Enzo, AU - Di Leo,Alfredo, PY - 2004/4/8/pubmed PY - 2004/7/16/medline PY - 2004/4/8/entrez SP - 165 EP - 72 JF - Helicobacter JO - Helicobacter VL - 9 IS - 2 N2 - BACKGROUND: Functional probiotics may prevent Helicobacter pylori infection, and some evidence suggests that they also possess antitumor properties. Lactobacillus brevis (CD2) is a functional Lactobacillus strain with peculiar biochemical features, essentially related to the activity of arginine deiminase. This enzyme catalyzes the catabolism of arginine and affects the biosynthesis of polyamines (putrescine, spermidine, and spermine). Polyamines are polycations found in high concentrations in both normal and neoplastic cells. Our aims were: 1, to assess whether oral administration of L. brevis (CD2) affects H. pylori survival in the human gastric mucosa; 2, to evaluate the effects of L. brevis (CD2) on polyamine biosynthesis in gastric biopsies from H. pylori-positive patients. MATERIALS AND METHODS: For 3 weeks before endoscopy, 22 H. pylori-positive dyspeptic patients randomly received (ratio 1 : 1) high oral doses of L. brevis (CD2) or placebo. Before and after treatment, H. pylori infection was determined by urea breath test (UBT). In gastric biopsies, ornithine decarboxylase activity and polyamine levels were, respectively, evaluated by a radiometric technique and high-pressure liquid chromatography (HPLC). RESULTS: L. brevis (CD2) treatment did not eradicate H. pylori. However, a reduction in the UBT delta values occurred, suggesting a decrease in intragastric bacterial load. Significantly, L. brevis (CD2) induced a decrease in gastric ornithine decarboxylase activity and polyamine levels. CONCLUSIONS: Our data support the hypothesis that L. brevis (CD2) treatment decreases H. pylori colonization, thus reducing polyamine biosynthesis. Alternatively, the arginine deiminase activity following L. brevis (CD2) administration might cause arginine deficiency, preventing polyamine generation from gastric cells. SN - 1083-4389 UR - https://www.unboundmedicine.com/medline/citation/15068419/The_influence_of_Lactobacillus_brevis_on_ornithine_decarboxylase_activity_and_polyamine_profiles_in_Helicobacter_pylori_infected_gastric_mucosa_ L2 - https://doi.org/10.1111/j.1083-4389.2004.00214.x DB - PRIME DP - Unbound Medicine ER -