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High DNA damage by benzo[a]pyrene 7,8-diol-9,10-epoxide in bronchial epithelial cells from patients with lung cancer: comparison with lung parenchyma.
Cancer Lett. 2004 Apr 30; 207(2):157-63.CL

Abstract

In the present study, the level of benzo[a]pyrene 7,8-diol-9,10-epoxide-N(2)-deoxyguanosine (BPDE-N(2)-dG) in normal bronchial epithelial cells from non-cancerous bronchus of 22 lung cancer subjects was evaluated and compared to the lung parenchyma. We found very high formation of BPDE-N(2)-dG adduct in samples corresponding to a pure preparation of bronchial epithelial cells with 4-fold interindividual differences in the DNA adduct levels in the range of 36.5-175.4 BPDE-N(2)-dG adducts/10(8) nucleotides in smokers (mean: 84.7+/-38.4; n = 13) and 3-fold differences in the range of 19.7-62.4 in non-smokers (mean: 37.6+/-22.2; n = 3). DNA isolated from the bronchial tissue consisting of bronchial lining epithelium with adjacent lamina propria showed significantly lower BPDE-N(2)-dG formation (P < 0.001) in the range of 0.4-4.2 BPDE-N(2)-dG adducts/10(8) nucleotides (mean: 1.8+/-0.56; n = 6). This difference is clearly related to the procedure used to prepare the bronchial tissue samples leading to the presence of different types of cells. Eight samples from the normal parenchyma did not show measurable adducts, the other 14 samples showed 50-fold variation (mean: 1.7+/-1.5; range 0.1-5.2 adducts/10(8) nucleotides; n = 14). There were considerably higher adduct levels in pure bronchial epithelial cells than in parenchymal tissue (75.8+/-38.8 vs 0.9+/-1.5 adducts/10(8) nucleotides) (P < 0.0002) BPDE-N(2)-dG adduct concentrate almost exclusively in bronchial epithelial cells. The adduct values obtained in bronchial epithelial cells could be considered as 'critical' for the initiation of human lung cancer. The high formation of BPDE-N(2)-dG adducts in bronchial epithelial cells and investigations showing that the profile of mutations induced by BPDE in these cells is similar to that seen in the p53 gene isolated from human lung tumors implicates benzo[a]pyrene as important carcinogen in tobacco-induced lung cancer in human beings.

Authors+Show Affiliations

EA3443 and Laboratoire d'Anatomie Pathologique, Faculté de Médecine, Université de Nancy, Av. de La Forêt de Haye, Vandoeuvre-Les-Nancy 54500, France.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15072824

Citation

Rojas, Margarita, et al. "High DNA Damage By Benzo[a]pyrene 7,8-diol-9,10-epoxide in Bronchial Epithelial Cells From Patients With Lung Cancer: Comparison With Lung Parenchyma." Cancer Letters, vol. 207, no. 2, 2004, pp. 157-63.
Rojas M, Marie B, Vignaud JM, et al. High DNA damage by benzo[a]pyrene 7,8-diol-9,10-epoxide in bronchial epithelial cells from patients with lung cancer: comparison with lung parenchyma. Cancer Lett. 2004;207(2):157-63.
Rojas, M., Marie, B., Vignaud, J. M., Martinet, N., Siat, J., Grosdidier, G., Cascorbi, I., & Alexandrov, K. (2004). High DNA damage by benzo[a]pyrene 7,8-diol-9,10-epoxide in bronchial epithelial cells from patients with lung cancer: comparison with lung parenchyma. Cancer Letters, 207(2), 157-63.
Rojas M, et al. High DNA Damage By Benzo[a]pyrene 7,8-diol-9,10-epoxide in Bronchial Epithelial Cells From Patients With Lung Cancer: Comparison With Lung Parenchyma. Cancer Lett. 2004 Apr 30;207(2):157-63. PubMed PMID: 15072824.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - High DNA damage by benzo[a]pyrene 7,8-diol-9,10-epoxide in bronchial epithelial cells from patients with lung cancer: comparison with lung parenchyma. AU - Rojas,Margarita, AU - Marie,Beatrice, AU - Vignaud,Jean Michel, AU - Martinet,Nadine, AU - Siat,Joëlle, AU - Grosdidier,Gilles, AU - Cascorbi,Ingolf, AU - Alexandrov,Kroum, PY - 2003/07/27/received PY - 2003/11/12/revised PY - 2003/11/13/accepted PY - 2004/4/10/pubmed PY - 2004/6/16/medline PY - 2004/4/10/entrez SP - 157 EP - 63 JF - Cancer letters JO - Cancer Lett VL - 207 IS - 2 N2 - In the present study, the level of benzo[a]pyrene 7,8-diol-9,10-epoxide-N(2)-deoxyguanosine (BPDE-N(2)-dG) in normal bronchial epithelial cells from non-cancerous bronchus of 22 lung cancer subjects was evaluated and compared to the lung parenchyma. We found very high formation of BPDE-N(2)-dG adduct in samples corresponding to a pure preparation of bronchial epithelial cells with 4-fold interindividual differences in the DNA adduct levels in the range of 36.5-175.4 BPDE-N(2)-dG adducts/10(8) nucleotides in smokers (mean: 84.7+/-38.4; n = 13) and 3-fold differences in the range of 19.7-62.4 in non-smokers (mean: 37.6+/-22.2; n = 3). DNA isolated from the bronchial tissue consisting of bronchial lining epithelium with adjacent lamina propria showed significantly lower BPDE-N(2)-dG formation (P < 0.001) in the range of 0.4-4.2 BPDE-N(2)-dG adducts/10(8) nucleotides (mean: 1.8+/-0.56; n = 6). This difference is clearly related to the procedure used to prepare the bronchial tissue samples leading to the presence of different types of cells. Eight samples from the normal parenchyma did not show measurable adducts, the other 14 samples showed 50-fold variation (mean: 1.7+/-1.5; range 0.1-5.2 adducts/10(8) nucleotides; n = 14). There were considerably higher adduct levels in pure bronchial epithelial cells than in parenchymal tissue (75.8+/-38.8 vs 0.9+/-1.5 adducts/10(8) nucleotides) (P < 0.0002) BPDE-N(2)-dG adduct concentrate almost exclusively in bronchial epithelial cells. The adduct values obtained in bronchial epithelial cells could be considered as 'critical' for the initiation of human lung cancer. The high formation of BPDE-N(2)-dG adducts in bronchial epithelial cells and investigations showing that the profile of mutations induced by BPDE in these cells is similar to that seen in the p53 gene isolated from human lung tumors implicates benzo[a]pyrene as important carcinogen in tobacco-induced lung cancer in human beings. SN - 0304-3835 UR - https://www.unboundmedicine.com/medline/citation/15072824/High_DNA_damage_by_benzo[a]pyrene_78_diol_910_epoxide_in_bronchial_epithelial_cells_from_patients_with_lung_cancer:_comparison_with_lung_parenchyma_ DB - PRIME DP - Unbound Medicine ER -