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Overexpression of HAP4 in glucose-derepressed yeast cells reveals respiratory control of glucose-regulated genes.
Microbiology (Reading). 2004 Apr; 150(Pt 4):929-934.M

Abstract

A link between control of respiration and glucose repression in yeast is reported. The HAP4 gene was overexpressed in a Delta mig1 deletion background, generating a mutant in which respiratory function is stimulated and glucose repression is diminished. Although this combination does not result in derepression of genes encoding proteins involved in respiratory function, it nevertheless generates resistance against 2-deoxyglucose and hence contributes to more derepressed growth characteristics. Unexpectedly, overexpression of HAP4 in the Delta mig1 deletion strain causes strong repression of several target genes of the Mig1p repressor. Repression is not restricted to glucose growth conditions and does not require the glucose repressors Mig2p or Hxk2p. It was observed that expression of the SUC2 gene is transiently repressed after glucose is added to respiratory-growing Delta mig1 cells. Additional overexpression of HAP4 prevents release from this novel repressed state. The data presented show that respiratory function controls transcription of genes required for the metabolism of alternative sugars. This respiratory feedback control is suggested to regulate the feed into glycolysis in derepressed conditions.

Authors+Show Affiliations

Swammerdam Institute for Life Sciences, University of Amsterdam, Kruislaan 318, 1098 SM Amsterdam, The Netherlands.Swammerdam Institute for Life Sciences, University of Amsterdam, Kruislaan 318, 1098 SM Amsterdam, The Netherlands.Swammerdam Institute for Life Sciences, University of Amsterdam, Kruislaan 318, 1098 SM Amsterdam, The Netherlands.Swammerdam Institute for Life Sciences, University of Amsterdam, Kruislaan 318, 1098 SM Amsterdam, The Netherlands.Swammerdam Institute for Life Sciences, University of Amsterdam, Kruislaan 318, 1098 SM Amsterdam, The Netherlands.Swammerdam Institute for Life Sciences, University of Amsterdam, Kruislaan 318, 1098 SM Amsterdam, The Netherlands.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15073302

Citation

Lascaris, Romeo, et al. "Overexpression of HAP4 in Glucose-derepressed Yeast Cells Reveals Respiratory Control of Glucose-regulated Genes." Microbiology (Reading, England), vol. 150, no. Pt 4, 2004, pp. 929-934.
Lascaris R, Piwowarski J, van der Spek H, et al. Overexpression of HAP4 in glucose-derepressed yeast cells reveals respiratory control of glucose-regulated genes. Microbiology (Reading). 2004;150(Pt 4):929-934.
Lascaris, R., Piwowarski, J., van der Spek, H., de Mattos, J. T., Grivell, L., & Blom, J. (2004). Overexpression of HAP4 in glucose-derepressed yeast cells reveals respiratory control of glucose-regulated genes. Microbiology (Reading, England), 150(Pt 4), 929-934. https://doi.org/10.1099/mic.0.26742-0
Lascaris R, et al. Overexpression of HAP4 in Glucose-derepressed Yeast Cells Reveals Respiratory Control of Glucose-regulated Genes. Microbiology (Reading). 2004;150(Pt 4):929-934. PubMed PMID: 15073302.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Overexpression of HAP4 in glucose-derepressed yeast cells reveals respiratory control of glucose-regulated genes. AU - Lascaris,Romeo, AU - Piwowarski,Jan, AU - van der Spek,Hans, AU - de Mattos,Joost Teixeira, AU - Grivell,Les, AU - Blom,Jolanda, PY - 2004/4/10/pubmed PY - 2004/7/10/medline PY - 2004/4/10/entrez SP - 929 EP - 934 JF - Microbiology (Reading, England) JO - Microbiology (Reading) VL - 150 IS - Pt 4 N2 - A link between control of respiration and glucose repression in yeast is reported. The HAP4 gene was overexpressed in a Delta mig1 deletion background, generating a mutant in which respiratory function is stimulated and glucose repression is diminished. Although this combination does not result in derepression of genes encoding proteins involved in respiratory function, it nevertheless generates resistance against 2-deoxyglucose and hence contributes to more derepressed growth characteristics. Unexpectedly, overexpression of HAP4 in the Delta mig1 deletion strain causes strong repression of several target genes of the Mig1p repressor. Repression is not restricted to glucose growth conditions and does not require the glucose repressors Mig2p or Hxk2p. It was observed that expression of the SUC2 gene is transiently repressed after glucose is added to respiratory-growing Delta mig1 cells. Additional overexpression of HAP4 prevents release from this novel repressed state. The data presented show that respiratory function controls transcription of genes required for the metabolism of alternative sugars. This respiratory feedback control is suggested to regulate the feed into glycolysis in derepressed conditions. SN - 1350-0872 UR - https://www.unboundmedicine.com/medline/citation/15073302/Overexpression_of_HAP4_in_glucose_derepressed_yeast_cells_reveals_respiratory_control_of_glucose_regulated_genes_ DB - PRIME DP - Unbound Medicine ER -