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Comparative serological evaluation of 10 Blastomyces dermatitidis yeast phase lysate antigens from different sources.
Mycoses. 2004 Apr; 47(3-4):143-9.M

Abstract

Yeast phase lysate antigens from 10 isolates of Blastomyces dermatitidis (dog, ERC-2 and T-58; T-27, polar bear; woodpile, ER-3; bat lung, 48938; human, B5929, B5895, B5896, B5931, and CAPP) from different geographical regions, in addition to a Histoplasma capsulatum (G217B) lysate preparation were compared with respect to their reactivity against serum specimens from dogs, rabbits and humans positive for blastomycosis using an indirect enzyme-linked immunosorbent assay. In addition, the lysate antigens were also assayed against histoplasmosis-positive human serum samples to study their cross-reactivity. Variable results were obtained with T-58 and T-27 exhibiting the greatest reactivity. We also noticed that the lysate did not react consistently to serum samples across species with lesser reactivity evidenced when testing dog sera. Finally, T-58 gave the highest cross-reactivity with histoplasmosis-positive sera. The study may prove valuable in the development of antigen candidates for blastomycosis serodiagnosis.

Authors+Show Affiliations

Abuodeh RO
College of Health Sciences, University of Sharjah, Sharjah, United Arab Emirates. abuodeh@sharjah.ac.ae
Chester EM
No affiliation info available
Scalarone GM
No affiliation info available

MeSH

AnimalsAntibodies, FungalAntigens, FungalBlastomycesBlastomycosisCross ReactionsDog DiseasesDogsEnzyme-Linked Immunosorbent AssayHumansImmune SeraRabbitsUrsidae

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15078431

Citation

Abuodeh, R O., et al. "Comparative Serological Evaluation of 10 Blastomyces Dermatitidis Yeast Phase Lysate Antigens From Different Sources." Mycoses, vol. 47, no. 3-4, 2004, pp. 143-9.
Abuodeh RO, Chester EM, Scalarone GM. Comparative serological evaluation of 10 Blastomyces dermatitidis yeast phase lysate antigens from different sources. Mycoses. 2004;47(3-4):143-9.
Abuodeh, R. O., Chester, E. M., & Scalarone, G. M. (2004). Comparative serological evaluation of 10 Blastomyces dermatitidis yeast phase lysate antigens from different sources. Mycoses, 47(3-4), 143-9.
Abuodeh RO, Chester EM, Scalarone GM. Comparative Serological Evaluation of 10 Blastomyces Dermatitidis Yeast Phase Lysate Antigens From Different Sources. Mycoses. 2004;47(3-4):143-9. PubMed PMID: 15078431.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Comparative serological evaluation of 10 Blastomyces dermatitidis yeast phase lysate antigens from different sources. AU - Abuodeh,R O, AU - Chester,E M, AU - Scalarone,G M, PY - 2004/4/14/pubmed PY - 2004/9/3/medline PY - 2004/4/14/entrez SP - 143 EP - 9 JF - Mycoses JO - Mycoses VL - 47 IS - 3-4 N2 - Yeast phase lysate antigens from 10 isolates of Blastomyces dermatitidis (dog, ERC-2 and T-58; T-27, polar bear; woodpile, ER-3; bat lung, 48938; human, B5929, B5895, B5896, B5931, and CAPP) from different geographical regions, in addition to a Histoplasma capsulatum (G217B) lysate preparation were compared with respect to their reactivity against serum specimens from dogs, rabbits and humans positive for blastomycosis using an indirect enzyme-linked immunosorbent assay. In addition, the lysate antigens were also assayed against histoplasmosis-positive human serum samples to study their cross-reactivity. Variable results were obtained with T-58 and T-27 exhibiting the greatest reactivity. We also noticed that the lysate did not react consistently to serum samples across species with lesser reactivity evidenced when testing dog sera. Finally, T-58 gave the highest cross-reactivity with histoplasmosis-positive sera. The study may prove valuable in the development of antigen candidates for blastomycosis serodiagnosis. SN - 0933-7407 UR - https://www.unboundmedicine.com/medline/citation/15078431/Comparative_serological_evaluation_of_10_Blastomyces_dermatitidis_yeast_phase_lysate_antigens_from_different_sources_ DB - PRIME DP - Unbound Medicine ER -