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Genomic characterization of Saccharomyces cerevisiae strains isolated from wine-producing areas in South America.
J Appl Microbiol. 2004; 96(5):1161-8.JA

Abstract

AIMS

The wide use of yeast inoculum for wine fermentations permit the spreading of commercial Saccharomyces strains in wine areas all over the world. To study the impact of this practice on the autochthonous yeast populations it is necessary to have tools that permit the evaluation of the geographical origin of native isolates and differentiate them from commercial strains.

METHODS AND RESULTS

Electrophoretic karyotyping and mitochondrial DNA restriction analysis were used to characterize the genome of native S. cerevisiae isolates associated to wine from three countries in South America. Both methods revealed differences in the genomic structure between these populations, in addition to differences between sub-populations collected in wine-producing areas in Chile.

CONCLUSIONS

Our data support that molecular polymorphism analysis may be useful to evaluate the geographical origin of native isolates of yeast strains for industrial use. Furthermore, these findings are in agreement with the idea of a clonal mode of reproduction of wine yeasts in natural environments.

SIGNIFICANCE AND IMPACT OF THE STUDY

This study permits the characterization of native yeast isolates in relation to their geographical origin. This procedure could be used as a tool for evaluating if a native isolate derives from the region were it was collected or if it is a strain derived from a commercial strain by microevolution.

Authors+Show Affiliations

Martínez C
Centro de Estudios en Ciencia y Tecnología de Alimentos (CECTA), Universidad de Santiago de Chile, Chile. cmartine@usach.cl
Gac S
No affiliation info available
Lavín A
No affiliation info available
Ganga M
No affiliation info available

MeSH

BiodiversityChileChromosomes, FungalDNA FingerprintingDNA, FungalElectrophoresis, Gel, Pulsed-FieldFood MicrobiologyGenotypePeruPolymorphism, Restriction Fragment LengthSaccharomyces cerevisiaeUruguayWine

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15078534

Citation

Martínez, C, et al. "Genomic Characterization of Saccharomyces Cerevisiae Strains Isolated From Wine-producing Areas in South America." Journal of Applied Microbiology, vol. 96, no. 5, 2004, pp. 1161-8.
Martínez C, Gac S, Lavín A, et al. Genomic characterization of Saccharomyces cerevisiae strains isolated from wine-producing areas in South America. J Appl Microbiol. 2004;96(5):1161-8.
Martínez, C., Gac, S., Lavín, A., & Ganga, M. (2004). Genomic characterization of Saccharomyces cerevisiae strains isolated from wine-producing areas in South America. Journal of Applied Microbiology, 96(5), 1161-8.
Martínez C, et al. Genomic Characterization of Saccharomyces Cerevisiae Strains Isolated From Wine-producing Areas in South America. J Appl Microbiol. 2004;96(5):1161-8. PubMed PMID: 15078534.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Genomic characterization of Saccharomyces cerevisiae strains isolated from wine-producing areas in South America. AU - Martínez,C, AU - Gac,S, AU - Lavín,A, AU - Ganga,M, PY - 2004/4/14/pubmed PY - 2004/7/31/medline PY - 2004/4/14/entrez SP - 1161 EP - 8 JF - Journal of applied microbiology JO - J Appl Microbiol VL - 96 IS - 5 N2 - AIMS: The wide use of yeast inoculum for wine fermentations permit the spreading of commercial Saccharomyces strains in wine areas all over the world. To study the impact of this practice on the autochthonous yeast populations it is necessary to have tools that permit the evaluation of the geographical origin of native isolates and differentiate them from commercial strains. METHODS AND RESULTS: Electrophoretic karyotyping and mitochondrial DNA restriction analysis were used to characterize the genome of native S. cerevisiae isolates associated to wine from three countries in South America. Both methods revealed differences in the genomic structure between these populations, in addition to differences between sub-populations collected in wine-producing areas in Chile. CONCLUSIONS: Our data support that molecular polymorphism analysis may be useful to evaluate the geographical origin of native isolates of yeast strains for industrial use. Furthermore, these findings are in agreement with the idea of a clonal mode of reproduction of wine yeasts in natural environments. SIGNIFICANCE AND IMPACT OF THE STUDY: This study permits the characterization of native yeast isolates in relation to their geographical origin. This procedure could be used as a tool for evaluating if a native isolate derives from the region were it was collected or if it is a strain derived from a commercial strain by microevolution. SN - 1364-5072 UR - https://www.unboundmedicine.com/medline/citation/15078534/Genomic_characterization_of_Saccharomyces_cerevisiae_strains_isolated_from_wine_producing_areas_in_South_America_ DB - PRIME DP - Unbound Medicine ER -