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Smallpox DNA vaccine protects nonhuman primates against lethal monkeypox.
J Virol. 2004 May; 78(9):4433-43.JV

Abstract

Two decades after a worldwide vaccination campaign was used to successfully eradicate naturally occurring smallpox, the threat of bioterrorism has led to renewed vaccination programs. In addition, sporadic outbreaks of human monkeypox in Africa and a recent outbreak of human monkeypox in the U.S. have made it clear that naturally occurring zoonotic orthopoxvirus diseases remain a public health concern. Much of the threat posed by orthopoxviruses could be eliminated by vaccination; however, because the smallpox vaccine is a live orthopoxvirus vaccine (vaccinia virus) administered to the skin, the vaccine itself can pose a serious health risk. Here, we demonstrate that rhesus macaques vaccinated with a DNA vaccine consisting of four vaccinia virus genes (L1R, A27L, A33R, and B5R) were protected from severe disease after an otherwise lethal challenge with monkeypox virus. Animals vaccinated with a single gene (L1R) which encodes a target of neutralizing antibodies developed severe disease but survived. This is the first demonstration that a subunit vaccine approach to smallpox-monkeypox immunization is feasible.

Authors+Show Affiliations

Virology Division, United States Army Medical Research Institute of Infectious Diseases, Fort Detrick, Maryland 21702, USA. jay.hooper@amedd.army.milNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

15078924

Citation

Hooper, J W., et al. "Smallpox DNA Vaccine Protects Nonhuman Primates Against Lethal Monkeypox." Journal of Virology, vol. 78, no. 9, 2004, pp. 4433-43.
Hooper JW, Thompson E, Wilhelmsen C, et al. Smallpox DNA vaccine protects nonhuman primates against lethal monkeypox. J Virol. 2004;78(9):4433-43.
Hooper, J. W., Thompson, E., Wilhelmsen, C., Zimmerman, M., Ichou, M. A., Steffen, S. E., Schmaljohn, C. S., Schmaljohn, A. L., & Jahrling, P. B. (2004). Smallpox DNA vaccine protects nonhuman primates against lethal monkeypox. Journal of Virology, 78(9), 4433-43.
Hooper JW, et al. Smallpox DNA Vaccine Protects Nonhuman Primates Against Lethal Monkeypox. J Virol. 2004;78(9):4433-43. PubMed PMID: 15078924.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Smallpox DNA vaccine protects nonhuman primates against lethal monkeypox. AU - Hooper,J W, AU - Thompson,E, AU - Wilhelmsen,C, AU - Zimmerman,M, AU - Ichou,M Ait, AU - Steffen,S E, AU - Schmaljohn,C S, AU - Schmaljohn,A L, AU - Jahrling,P B, PY - 2004/4/14/pubmed PY - 2004/6/2/medline PY - 2004/4/14/entrez SP - 4433 EP - 43 JF - Journal of virology JO - J Virol VL - 78 IS - 9 N2 - Two decades after a worldwide vaccination campaign was used to successfully eradicate naturally occurring smallpox, the threat of bioterrorism has led to renewed vaccination programs. In addition, sporadic outbreaks of human monkeypox in Africa and a recent outbreak of human monkeypox in the U.S. have made it clear that naturally occurring zoonotic orthopoxvirus diseases remain a public health concern. Much of the threat posed by orthopoxviruses could be eliminated by vaccination; however, because the smallpox vaccine is a live orthopoxvirus vaccine (vaccinia virus) administered to the skin, the vaccine itself can pose a serious health risk. Here, we demonstrate that rhesus macaques vaccinated with a DNA vaccine consisting of four vaccinia virus genes (L1R, A27L, A33R, and B5R) were protected from severe disease after an otherwise lethal challenge with monkeypox virus. Animals vaccinated with a single gene (L1R) which encodes a target of neutralizing antibodies developed severe disease but survived. This is the first demonstration that a subunit vaccine approach to smallpox-monkeypox immunization is feasible. SN - 0022-538X UR - https://www.unboundmedicine.com/medline/citation/15078924/Smallpox_DNA_vaccine_protects_nonhuman_primates_against_lethal_monkeypox_ L2 - https://journals.asm.org/doi/10.1128/jvi.78.9.4433-4443.2004?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -