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Flow cytometric immunophenotypic analysis of 306 cases of multiple myeloma.
Am J Clin Pathol. 2004 Apr; 121(4):482-8.AJ

Abstract

Bone marrow aspirates from 306 patients with multiple myeloma were analyzed by flow cytometric immunophenotyping. The plasma cells (PCs) were identified by their characteristic light scatter distribution and reactivity patterns to CD138, CD38, and CD45. Monoclonality was confirmed by immunoglobulin light chain analysis. The immunophenotypic profile of the PCs was determined with a panel of antibodies. Moderate to bright expression of CD56, CD117, CD20, CD45, and CD52 was detected in 71.7%, 17.8%, 9.3%, 8.8%, and 5.2% of cases, respectively. These antigens were expressed by a distinct subpopulation of the PCs in 6.3%, 2.2%, 3.7%, 2.9%, and 2.6% of additional cases. CD19 was negative in more than 99% of cases. The combination of CD38 and CD138 was superior to CD38 alone for identifying CD45+ myeloma and separating CD20+ myeloma from B-cell lymphoma. PC immunophenotyping might be useful for detecting minimal residual disease in cases with aberrant antigen expression and for selection of therapeutic agents that have specific membrane targets.

Authors+Show Affiliations

Department of Pathology and Laboratory Medicine, University of Arkansas for Medical Sciences, Little Rock, USA.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article

Language

eng

PubMed ID

15080299

Citation

Lin, Pei, et al. "Flow Cytometric Immunophenotypic Analysis of 306 Cases of Multiple Myeloma." American Journal of Clinical Pathology, vol. 121, no. 4, 2004, pp. 482-8.
Lin P, Owens R, Tricot G, et al. Flow cytometric immunophenotypic analysis of 306 cases of multiple myeloma. Am J Clin Pathol. 2004;121(4):482-8.
Lin, P., Owens, R., Tricot, G., & Wilson, C. S. (2004). Flow cytometric immunophenotypic analysis of 306 cases of multiple myeloma. American Journal of Clinical Pathology, 121(4), 482-8.
Lin P, et al. Flow Cytometric Immunophenotypic Analysis of 306 Cases of Multiple Myeloma. Am J Clin Pathol. 2004;121(4):482-8. PubMed PMID: 15080299.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Flow cytometric immunophenotypic analysis of 306 cases of multiple myeloma. AU - Lin,Pei, AU - Owens,Rebecca, AU - Tricot,Guido, AU - Wilson,Carla S, PY - 2004/4/15/pubmed PY - 2004/5/7/medline PY - 2004/4/15/entrez SP - 482 EP - 8 JF - American journal of clinical pathology JO - Am. J. Clin. Pathol. VL - 121 IS - 4 N2 - Bone marrow aspirates from 306 patients with multiple myeloma were analyzed by flow cytometric immunophenotyping. The plasma cells (PCs) were identified by their characteristic light scatter distribution and reactivity patterns to CD138, CD38, and CD45. Monoclonality was confirmed by immunoglobulin light chain analysis. The immunophenotypic profile of the PCs was determined with a panel of antibodies. Moderate to bright expression of CD56, CD117, CD20, CD45, and CD52 was detected in 71.7%, 17.8%, 9.3%, 8.8%, and 5.2% of cases, respectively. These antigens were expressed by a distinct subpopulation of the PCs in 6.3%, 2.2%, 3.7%, 2.9%, and 2.6% of additional cases. CD19 was negative in more than 99% of cases. The combination of CD38 and CD138 was superior to CD38 alone for identifying CD45+ myeloma and separating CD20+ myeloma from B-cell lymphoma. PC immunophenotyping might be useful for detecting minimal residual disease in cases with aberrant antigen expression and for selection of therapeutic agents that have specific membrane targets. SN - 0002-9173 UR - https://www.unboundmedicine.com/medline/citation/15080299/Flow_cytometric_immunophenotypic_analysis_of_306_cases_of_multiple_myeloma_ L2 - https://academic.oup.com/ajcp/article-lookup/doi/10.1309/74R4-TB90-BUWH-27JX DB - PRIME DP - Unbound Medicine ER -