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Detection of severe acute respiratory syndrome-associated coronavirus in pneumocytes of the lung.
Am J Clin Pathol. 2004 Apr; 121(4):574-80.AJ

Abstract

Previous reports have indicated that patients with severe acute respiratory syndrome (SARS)-associated coronavirus infection could develop atypical pneumonia with fulminant pulmonary edema. However, the target cells of SARS viral infection have not been characterized in detail. We report the pathologic findings of the lung in 3 cases of SARS. Chest radiographs at 2 to 3 weeks of infection revealed an atypical pneumonia with pulmonary consolidation, a clinical characteristic of SARS infection. The presence of the SARS virus was determined by nested reverse transcription-polymerase chain reaction (RT-PCR), and the infected cells were identified by in situ hybridization in open-lung biopsy and postmortem necropsy specimens. Expression of SARS virus-encoded RNA was detected in all 3 cases by RT-PCR, and the SARS viral signal was localized in pneumocytes by using in situ hybridization.

Authors+Show Affiliations

Institute of Biomedical Sciences, National Chung Hsing University, Taichung, Taiwan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Case Reports
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15080310

Citation

Chow, Kuan-Chih, et al. "Detection of Severe Acute Respiratory Syndrome-associated Coronavirus in Pneumocytes of the Lung." American Journal of Clinical Pathology, vol. 121, no. 4, 2004, pp. 574-80.
Chow KC, Hsiao CH, Lin TY, et al. Detection of severe acute respiratory syndrome-associated coronavirus in pneumocytes of the lung. Am J Clin Pathol. 2004;121(4):574-80.
Chow, K. C., Hsiao, C. H., Lin, T. Y., Chen, C. L., & Chiou, S. H. (2004). Detection of severe acute respiratory syndrome-associated coronavirus in pneumocytes of the lung. American Journal of Clinical Pathology, 121(4), 574-80.
Chow KC, et al. Detection of Severe Acute Respiratory Syndrome-associated Coronavirus in Pneumocytes of the Lung. Am J Clin Pathol. 2004;121(4):574-80. PubMed PMID: 15080310.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Detection of severe acute respiratory syndrome-associated coronavirus in pneumocytes of the lung. AU - Chow,Kuan-Chih, AU - Hsiao,Cheng-Hsiang, AU - Lin,Tze-Yi, AU - Chen,Chi-Long, AU - Chiou,Shiow-Her, PY - 2004/4/15/pubmed PY - 2004/5/7/medline PY - 2004/4/15/entrez SP - 574 EP - 80 JF - American journal of clinical pathology JO - Am J Clin Pathol VL - 121 IS - 4 N2 - Previous reports have indicated that patients with severe acute respiratory syndrome (SARS)-associated coronavirus infection could develop atypical pneumonia with fulminant pulmonary edema. However, the target cells of SARS viral infection have not been characterized in detail. We report the pathologic findings of the lung in 3 cases of SARS. Chest radiographs at 2 to 3 weeks of infection revealed an atypical pneumonia with pulmonary consolidation, a clinical characteristic of SARS infection. The presence of the SARS virus was determined by nested reverse transcription-polymerase chain reaction (RT-PCR), and the infected cells were identified by in situ hybridization in open-lung biopsy and postmortem necropsy specimens. Expression of SARS virus-encoded RNA was detected in all 3 cases by RT-PCR, and the SARS viral signal was localized in pneumocytes by using in situ hybridization. SN - 0002-9173 UR - https://www.unboundmedicine.com/medline/citation/15080310/Detection_of_severe_acute_respiratory_syndrome_associated_coronavirus_in_pneumocytes_of_the_lung_ L2 - https://academic.oup.com/ajcp/article-lookup/doi/10.1309/C0ED-U0RA-QBTX-BHCE DB - PRIME DP - Unbound Medicine ER -